Rat il 6 elisa kit

Manufactured by Abcam

Sourced in United States, United Kingdom

The Rat IL-6 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of rat interleukin-6 (IL-6) in cell culture supernatants, serum, and plasma samples.

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26 protocols using rat il 6 elisa kit

Quantifying Inflammatory Cytokines in Rat Serum

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ELISA was used to measure inflammatory cytokines in serum or cell supernatants. After the rats were euthanized, blood samples (2 ml) were collected in tubes without EDTA to obtain the serum. The supernatant was obtained after centrifugation at 1800 x g for 10 min at room temperature. Serum levels of TNF-α, IL-6, IL-1β, and MCP-1 were measured using commercial ELISA kits, including a rat TNF-α ELISA Kit (ab46070) (Abcam, Cambridge, MA, USA), a rat IL-6 ELISA Kit (ab234570) (Abcam, Cambridge, MA, USA), a rat IL-1β ELISA Kit (ab100767) (Abcam, Cambridge, MA, USA), and a rat MCP-1 ELISA Kit (ab219045) (Abcam, Cambridge, MA, USA), according to manufacturer’s instruction.

Liu K.H., Zhou N., Zou Y., Yang Y.Y., OuYang S.X, & Liang Y.M. (2019). Spleen Tyrosine Kinase (SYK) in the Progression of Peritoneal Fibrosis Through Activation of the TGF-β1/Smad3 Signaling Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 9346-9356.

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Measuring Serum Cytokine Levels in Rats

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The serum concentrations of pro-inflammatory cytokines in the rats were measured by ELISA, according to the manufacturer’s instructions. The ELISA kits used for detection of serum levels of cytokines included a rat interleukin (IL)-1β ELISA kit (Abcam, Cambridge, UK), a rat IL-6 ELISA kit (Abcam, Cambridge, UK), a rat IL-18 ELISA kit (Abcam, Cambridge, UK), a rat JE/CCL2/MCP-1 Duoset ELISA kit (R&D Systems, Minneapolis, MN, USA), and a rat tumor necrosis factor-α (TNF-α) ELISA kit (Abcam, Cambridge, UK).

Gulan T., Yeernuer T., Sui S, & Mayinuer N. (2019). A Rat Model of Maternal Polycystic Ovary Syndrome Shows that Exposure to Androgens In Utero Results in Dysbiosis of the Intestinal Microbiota and Metabolic Disorders of the Newborn Rat. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 9377-9391.

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PLGA-based Drug Delivery System Development

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PLGA (lactic acid:glycolic acid = 50:50; MW = 48,000) was obtained from Evonik Industries (Essen, Germany). Dichloromethane (DCM; >99.5%) and N,N‐dimethylformamide (DMF, >99.5%) were purchased from DaeJung (Siheung, Korea). Acetonitrile (ACN; >99.9%) and lidocaine were obtained from J. T. Bakers (Phillisburg, NJ) and Tokyo Chemical Industry (Tokyo, Japan), respectively. Polethylene catheters (SP‐45 and SP‐61) were obtained from Natsume Seisakusho (Tokyo, Japan). Surgical sutures (4–0 and 5–0 silk) and cotton bandages were purchased from Ailee (Seoul, Korea) and Bandgold (Ansan, Gyeonggi‐do, Korea), respectively. Cyclophosphamide (CYP) was purchased from Sigma‐Aldrich (MO). Anti‐COX‐2 polyclonal antibody (ab 15191), anti‐IL‐6 polyclonal antibody (ab 6672), anti‐NGF polyclonal antibody (ab 6199), and rat IL‐6 ELISA Kit (ab 234570) were obtained from Abcam (MA). DAB kit was purchased from Vector Laboratories (CA). Hematoxylin‐1 and bluing solution were purchased from the Korea Standard (Seongnam, Korea). Revoscript rt premix (25086) was purchased from Invitrogen (Carlsbad, CA). LightCycler ® 480 SYBR Green was purchased from Roche (Basel, Switzerland).

Kim C.R., Jang E.B., Hong S.H., Yoon Y.E., Huh B.K., Kim S.N., Kim M.J., Moon H.S, & Choy Y.B. (2021). Indwelling urinary catheter assembled with lidocaine‐loaded polymeric strand for local sustained alleviation of bladder discomfort. Bioengineering & Translational Medicine, 6(2), e10218.

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PLGA-based Inflammatory Cytokine ELISA Assay

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Standards of TPL (the purity is above 98.5% determined by HPLC-UV, China National Institutes for Food and Drug Control); PLGA (molecular weight = 15,000 Da, Shandong institute of medical devices); methanol (Merck Co., Gradient Grade), acetonitrile (Merck Co., Gradient Grade), ammonium formate (Merck Co., Gradient Grade); Rat IL-1α ELISA Kit, Rat IL-6 ELISA Kit, Rat TNF alpha ELISA Kit (Abcam^®); Rat CRP (C-Reactive Protein) ELISA Kit (Elabscience^®); anhydrous ethanol, paraformaldehyde, xylene, formaldehyde, hydrochloric acid, neutral balsam, propylene glycol (Sinopharm Chemical Reagent Co., Ltd., analytical grade); Chicken type II collagen (Hefei Bomei biotechnology Co., Ltd.); Phosphate buffer solution (PBS, Life Technologies^®).

Wang L., Ding Y., Tang Q, & Niu X. (2023). Preparation, Properties and Therapeutic Effect of a TPL Nanoparticle Thermosensitive Gel for Intra-Articular Injection. Molecules, 28(12), 4659.

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Quantifying IL-6 and IL-1β in LPS-treated H9c2 Cells

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Levels of two proinflammatory factors interleukin-6 (IL-6) and interleukin-1β (IL-1β) in LPS-induced H9c2 cells with or without transfection of miR-197 inhibitor were determined by an ELISA. Specifically, the rat IL-1β ELISA kit (ab255730, Abcam, USA) was added into the cells according to the protocol booklet. Then, the cells were sealed and incubated at room temperature for 1 h. After being washed with Wash Buffer PT three times, the cells were treated with 100 μl of TMB development solution for 10 minutes in the dark and with 100 μl stop solution for 1 minute. Following this, the final absorbance was measured at 450 nm using the microplate reader. Ultimately, the IL-6 level was determined by the rat IL-6 ELISA kit (ab234570, Abcam, USA) following the same procedures as mentioned above.

Liu M., Zhang Y., Cao X., Shi T, & Yan Y. (2022). miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1. Cardiology Research and Practice, 2022, 7687154.

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Quantification of Inflammatory Proteins in Rat Hippocampus

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The protein levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) in the hippocampus were determined using ELISA kits. The ELISA was performed according to the manufacturer’s instructions. Briefly, the hippocampus was collected and homogenized in ice-cold RIPA buffer (89900, Pierce, Waltham, MA, USA). The lysate was centrifugated and the supernatant was collected. The total protein concentrate was measured using the BCA method (23227, Pierce, Waltham, MA, USA). The IL-1β, IL-6, IL-10 and TNF-α protein levels were determined by Rat IL-1β ELISA kit (ab100768, Abcam, Boston, MA, USA), Rat IL-6 ELISA kit (ab100772, Abcam, Boston, MA, USA), Rat TNF-α ELISA kit (ab46070, Abcam, Boston, MA, USA) and Rat IL-10 ELISA kit (ab100765, Abcam, Boston, MA, USA), respectively. The optical density was measured by a microplate reader (xMark^TM, Bio-Rad, Hercules, CA, USA). Finally, the protein levels of inflammatory factors were conducted by normalizing them to total protein levels.

Guo Y., Yan M., Li L., Zhao L, & Li Y. (2022). Treadmill Exercise Prevents Cognitive Impairments in Adolescent Intermittent Ethanol Rats by Reducing the Excessive Activation of Microglia Cell in the Hippocampus. International Journal of Molecular Sciences, 23(23), 14701.

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Quantifying Inflammatory Biomarkers

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The plasma interleukin 6 (IL-6) and C-reactive protein (CRP) levels were measured by means of commercially available Rat CRP ELISA kit (Abcam, Cambridge, MA) and Rat IL-6 ELISA kit (Abcam, Cambridge, MA), respectively. All the procedures and conditions were consistent with the instructions of these kits.

Xu J., Gao H., Zhang L., Chen C., Yang W., Deng Q., Huang Q, & Huang F. (2014). A combination of flaxseed oil and astaxanthin alleviates atherosclerosis risk factors in high fat diet fed rats. Lipids in Health and Disease, 13, 63.

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Quantifying Inflammatory Biomarkers

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Xu J., Ma C., Han L., Gao H., Zhou Q., Yang M., Chen C., Deng Q., Huang Q, & Huang F. (2014). Optimized rapeseed oils rich in endogenous micronutrients ameliorate risk factors of atherosclerosis in high fat diet fed rats. Lipids in Health and Disease, 13, 166.

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Cytokine and Enzyme Profiling in Serum

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The concentration of serum TNF-α and IL-6 was determined by the rat TNF-α ELISA kit (Abcam) and the rat IL-6 ELISA kit (Abcam). In accordance with the instructions of the manufacturer, the detection of serum lipase and amylase content was carried out by the Olympus AU600 automatic biochemical analyzer (Tokyo, Japan).

Wang H., Li C., Jiang Y., Li H, & Zhang D. (2020). Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice, 2020, 8953453.

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Cytokine Quantification in Serum and BALF

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The levels of cytokines, including tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6, in the serum and BALF were tested using the following enzyme-linked immunosorbent assay (ELISA) kits: Rat TNF-α ELISA kit (ab100785, Abcam, Cambridge, UK), Rat IL-1β ELISA kit (ab100767, Abcam) and Rat IL-6 ELISA kit (ab100772, Abcam). All experiments were performed in accordance with the product instructions.

Xie Q., Wang Y, & Zou G.L. (2022). Protective effects of lavender oil on sepsis-induced acute lung injury via regulation of the NF-κB pathway. Pharmaceutical Biology, 60(1), 968-978.

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