N n dimethylformamide dmf trifluoroacetic acid tfa n methyl 2 pyrrolidone nmp

Manufactured by Biosolve

Sourced in Netherlands

N,N-dimethylformamide (DMF), trifluoroacetic acid (TFA), and N-methyl-2-pyrrolidone (NMP) are chemical solvents commonly used in various laboratory applications. DMF is a polar aprotic solvent, TFA is a strong organic acid, and NMP is a polar solvent. These substances have specific physicochemical properties that make them useful for specific laboratory procedures, but a detailed description of their intended use is not provided to maintain an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Triethoxysilane tes, by Merck Group (5 mentions) Dichloromethane dcm, by Biosolve (4 mentions) Piperidine, by Biosolve (4 mentions) N n diisopropylethylamine diea, by Biosolve (4 mentions) Acetonitrile, by Merck Group (2 mentions) Increlex 10 mg ml igf 1 solution, by Ipsen (2 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions) Hexafluoroisopropanol hfip, by Thermo Fisher Scientific (1 mentions) Pyridoxal 5 phosphate plp, by Merck Group (1 mentions) Polycaprolactone, by Merck Group (1 mentions) Cell strainer, by BD (1 mentions) Fibronectin, by BD (1 mentions) Tissue culture treated dishes, by BD (1 mentions) 1 1 1 3 3 3 hexafluoro 2 propanol hfip, by Merck Group (1 mentions) Endothelial cell growth medium mv2, by PromoCell (1 mentions) Dichloromethane, by Biosolve (1 mentions)

Close spelling variants of this product

Trifluoroacetic acid (TFA) (16 citations) Trifluoroacetic acid (14 citations) DMF (8 citations) Dimethylformamide (DMF) (4 citations) NMP (2 citations) N-Methyl-2-pyrrolidone (2 citations) Dimethylformamide (2 citations)

5 protocols using n n dimethylformamide dmf trifluoroacetic acid tfa n methyl 2 pyrrolidone nmp

Synthesis of Hyaluronic Acid Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hyaluronic acid (MW = 100–1250 kDa) was obtained from Contipro Biotech s.r.o (Dolni Dobrouc, Czech Republic). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and Triethoxysilane (TES) from Sigma Aldrich (Steinheim, Germany), and ethanol from VWR Chemicals Prolab (Fontenay-sous-Bois, France). The Rink Amide MBHA resin and the 9-fluorenylmethoxycarbonyl (Fmoc) protected amino acids were purchased from Novabiochem (Merck KGaA, Darmstadt, Germany). The coupling reagents 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and 1-Hydroxybenzotriazole (HOBt) from Advanced Biotech (Seveso, MI, Italy). N,N-diisopropylethylamine (DIEA) and piperidine were purchased from Biosolve (Leenderweg, Valkenswaard, The Netherlands). N,N-dimethylformamide (DMF), trifluoroacetic acid (TFA), N-methyl-2-pyrrolidone (NMP) and dichloromethane (DCM) were from Biosolve (Leenderweg, Valkenswaard, The Netherlands). Acetonitrile and TFA were from Sigma-Aldrich, Saint Louis, MO, USA.

Dettin M., Sieni E., Zamuner A., Marino R., Sgarbossa P., Lucibello M., Tosi A.L., Keller F., Campana L.G, & Signori E. (2019). A Novel 3D Scaffold for Cell Growth to Assess Electroporation Efficacy. Cells, 8(11), 1470.

+ Open protocol

+ Expand

Solid-Phase Peptide Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The solid support, Rink Amide MBHA resin, was from Novabiochem (Merck KGaA, Darmstadt, Germany). The Fluorenylmethyloxycarbonyl (Fmoc) protected amino acids were from Novabiochem. The coupling reagents 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), 1-Hydroxybenzotriazole (HOBt) and 3H-[1,2,3]-Triazolo[4,5-b]pyridin-3-ol (HOAt) were from Advanced Biotech (Seveso, Italy). N,N-diisopropylethylamine (DIEA) and piperidine were from Biosolve (Leenderweg, Valkenswaard, The Netherlands). 2,4,6-Collidine was from Janssen Chimica NV (Beerse, Belgium). Triethoxysilane (TES) was from Sigma-Aldrich (Steinheim, Germany). Solvents such as N,N-dimethylformamide (DMF), trifluoroacetic acid (TFA), N-methyl-2-pyrrolidone (NMP) and dichloromethane (DCM) were from Biosolve. Increlex (10 mg/mL IGF-1 solution) was from Ipsen Pharma (Boulogne-Billancourt, France).

Zamuner A., Cavo M., Scaglione S., Messina G.M., Russo T., Gloria A., Marletta G, & Dettin M. (2016). Design of Decorated Self-Assembling Peptide Hydrogels as Architecture for Mesenchymal Stem Cells. Materials, 9(9), 727.

+ Open protocol

+ Expand

Peptide Synthesis and Electrospun Scaffolds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The solid support (resin Rink-Amide MBHA) and the Fmoc protected amino acids were from Novabiochem (Merck KGaA, Darmstadt, Germany). The coupling reagents 2-(1H –Benzotriazole-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and 1-Hydroxybenzotriazole (HOBt) were from Advanced Biotech (Seveso, MI, Italy). N,N-diisopropylethylamine (DIEA) and piperidine were from Biosolve (Leenderweg, Valkenswaard, Netherlands). Triethoxysilane (TES), were from Sigma-Aldrich (Steinheim, Germany). Solvents such as N,N-dimethylformamide (DMF), trifluoroacetic acid (TFA), N-methyl-2-pyrrolidone (NMP) and dichloromethane (DCM) were from Biosolve (Leenderweg, Valkenswaard, Netherlands). The solvent for electrospinning solutions hexafluoroisopropanol (HFIP) was from Alfa Aesar (Ward Hill, MA, USA). Poly-ε-caprolactone, average M_n ca. 60,000 (PCL) used to produce the electrospun scaffolds was from Aldrich (St. Louis, MO, USA).

Dettin M., Zamuner A., Roso M., Gloria A., Iucci G., Messina G.M., D'Amora U., Marletta G., Modesti M., Castagliuolo I, & Brun P. (2015). Electrospun Scaffolds for Osteoblast Cells: Peptide-Induced Concentration-Dependent Improvements of Polycaprolactone. PLoS ONE, 10(9), e0137505.

+ Open protocol

+ Expand

Peptide Synthesis Using Rink Amide Resin

Check if the same lab product or an alternative is used in the 5 most similar protocols

The solid support, Rink Amide MBHA resin, was purchased from Novabiochem (Merck KGaA, Darmstadt, Germany). The Fmoc protected amino acids were provided by Novabiochem. The coupling reagents 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), 1-Hydroxybenzotriazole (HOBt) and 3H-[1,2,3]-Triazolo[4,5-b]pyridin-3-ol (HOAt) were supplied by Advanced Biotech (Seveso, MI, Italy). N,N-diisopropylethylamine (DIEA) and piperidine were purchased from Biosolve (Leenderweg, Valkenswaard, The Netherlands). 2,4,6-Collidine was from Janssen Chimica NV (Beerse, Belgium). Triethoxysilane (TES) and pyridoxal-5-phosphate (PLP) were from Sigma-Aldrich (Steinheim, Germany). Solvents such as N,N-dimethylformamide (DMF), trifluoroacetic acid (TFA), N-methyl-2-pyrrolidone (NMP) and dichloromethane (DCM) were from Biosolve. Increlex (10 mg/mL IGF-1 solution) was from Ipsen Pharma (Boulogne-Billancourt, France).

Brun P., Zamuner A., Peretti A., Conti J., Messina G.M., Marletta G, & Dettin M. (2019). 3D Synthetic Peptide-based Architectures for the Engineering of the Enteric Nervous System. Scientific Reports, 9, 5583.

+ Open protocol

+ Expand

Peptide Synthesis and Polymer Scaffold Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols

The solid support resins Sasrin and Amide MBHA were from Novabiochem (Merck KGaA, Darmstadt, Germany). The Fmoc protected amino acids were from Novabiochem (Merck KGaA, Darmstadt, Germany). The coupling reagents 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and 1-hydroxybenzotriazole (HOBt) were from Advanced Biotech (Seveso, MI, Italy). N,N-Diisopropylethylamine (DIEA) and piperidine were from Biosolve (Leenderweg, Valkenswaard, Netherlands). Triethoxysilane (TES) was from Sigma-Aldrich (Steinheim, Germany). Solvents such as N,N-dimethylformamide (DMF), trifluoroaceticacid (TFA), N-methyl-2-pyrrolidone (NMP), and dichloromethane (DCM) were from Biosolve (Leenderweg, Valkenswaard, Netherlands). Poly-ɛ-caprolactone (Mn = 60 KDa), acetonitrile, and 1,1,1-3,3,3-hexafluoro-2-propanol (HFIP) were purchased from Sigma-Aldrich (Steinheim, Germany). The copolymer poly(L-lactic acid-co-ɛ-caprolactone) (70 : 30) was purchased by PURAC biochem (Gorinchen, Holland).
Phosphate-buffered saline (PBS) tablets were purchased from Gibco Invitrogen Corp. (Paisley, UK). The Endothelial Cell Growth Medium MV2 was purchased from PromoCell GmbH (Heidelberg, Germany). Cell strainer, tissue culture-treated dishes, and fibronectin were from BD Biosciences (San Jose, CA, USA), whereas 3-(4,5-

Dettin M., Zamuner A., Roso M., Iucci G., Samouillan V., Danesin R., Modesti M, & Conconi M.T. (2015). Facile and selective covalent grafting of an RGD-peptide to electrospun scaffolds improves HUVEC adhesion. Journal of peptide science : an official publication of the European Peptide Society, 21(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!