Gemini nx

Manufactured by Phenomenex

Sourced in United States

The Gemini-NX is a high-performance, dual-column liquid chromatography system designed for improved productivity and efficiency in analytical laboratories. It features dual independent pumps, a switching valve, and a temperature-controlled column compartment to facilitate parallel analysis of samples.

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Lab products found in correlation

Bm 20alite prominence hplc system, by Shimadzu (2 mentions) Q tof premier high resolution mass spectrometer, by Waters Corporation (1 mentions) 1100 series hplc system, by Agilent Technologies (1 mentions) Itraq reagent 8 plex multiplex kit, by AB Sciex (1 mentions) Orbitrap elite, by Thermo Fisher Scientific (1 mentions) Proteome discoverer 2, by Thermo Fisher Scientific (1 mentions) Agilent 1100 hplc system, by Agilent Technologies (1 mentions) Water purification system, by Merck Group (1 mentions) Surveyor msq, by Thermo Fisher Scientific (1 mentions) Jupiter proteo 90a ax, by Phenomenex (1 mentions) Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions) Uv 3150, by Shimadzu (1 mentions) Scl 10avp controller, by Shimadzu (1 mentions) Ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions) Ultimate 3000 rslc, by Thermo Fisher Scientific (1 mentions) Api 2000 mass spectrometer, by Thermo Fisher Scientific (1 mentions) 1100 series hplc value system, by Agilent Technologies (1 mentions) 1100 series, by Agilent Technologies (1 mentions) C18 column, by Phenomenex (1 mentions)

32 protocols using gemini nx

Triazolyl-Isoquinolin-1(2H)-one Synthesis

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Example 15

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To a mixture of 6-(4-ethyl-3-(hydroxymethyl)-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl)-4-(prop-1-en-2-yl)isoquinolin-1(2H)-one (200 mg, 0.6 mmol) in DMF (6 mL) was added 2-methylphenyl boronic acid (304 mg, 2.2 mmol), Et₃N (227 mg, 2.2 mmol) and Cu(OAc)₂(102 mg, 0.6 mmol), respectively. The reaction mixture was stirred under O₂(15 Psi) at room temperature for 72 h. The reaction was quenched by the addition of saturated aqueous NH₄Cl solution. The mixture was extracted with EtOAc. The organic layer was separated, washed with brine, dried over Na₂SO₄, filtered, and concentrated. The residue was purified by preparative reversed phase HPLC (Stationary phase: Phenomenex Gemini-NX, 5 μm, 150×30 mm; Mobile phase: water (0.04% NH₃.H₂O+10 mM NH₄HCO₃) (A) -MeCN (B), gradient elution: 42-72% B in A over 8 min, flow rate: 25 mL/min) to give the title compound as a yellow solid (28 mg, yield: 12%). ESI-MS: mass calcd. for C₂₄H₂₄N₄O₃, 416.2; m/z found, 417.3 [M+H]⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.39 (d, J=1.71 Hz, 1H), 8.35 (d, J=8.80 Hz, 1H), 8.10 (dd, J=1.71, 8.80 Hz, 1H), 7.27-7.42 (m, 4H), 7.16 (s, 1H), 5.79 (t, J=5.87 Hz, 1H), 5.35 (s, 1H), 5.11 (s, 1H), 4.49 (d, J=5.87 Hz, 2H), 3.77 (q, J=7.09 Hz, 2H), 2.09 (s, 3H), 2.05 (s, 3H), 1.25 (t, J=7.09 Hz, 3H) ppm.

US11505536B2. Dihydroorotate dehydrogenase inhibitors (2022-11-22). Janssen Biotech, Inc. [US]. Inventors: Scott Kuduk [US], Zhuming Zhang [US], Lindsey DeRatt [US], Aihua Wang [US].

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Isolation and Characterization of Secondary Metabolites from Ralstonia solanacearum

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R. solanacearum GMI1000 cells were cultured in TTC medium for 48 h, and the supernatant was extracted with an equal volume of ethyl acetate. Following evaporation of the ethyl acetate, the residue was dissolved in methanol and subjected to HPLC analysis in a C18 reverse-phase column (XBridge, 10 μm, 19 mm × 250 mm, Waters) and eluted with methanol-water (from 35:65 to 100:0 v/v) at a flow rate of 7 ml/min. The active fractions were detected, concentrated, purified by HPLC using a semi-preparative C18 reverse-phase column (Gemini-NX, 5 μm, 10.0 mm × 250 mm, Phenomenex) and eluted with methanol-water (from 35:65 to 100:0 v/v) at a flow rate of 3 ml/min. Peaks were monitored using a ultraviolet (UV) detector at 210 nm and were collected and assayed. The experiment was performed according to the previous method [27 ].
The ¹H, ¹³C, and DEPT135 nuclear magnetic resonance (NMR) spectra in CD₃OD solution were obtained using a Bruker AV-500 (Bruker Instrument, Inc., Zurich, Switzerland) spectrometer operating at 500 MHz for ¹H or 125 MHz for ¹³C. High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) was performed on a Waters Q-Tof Premier high-resolution mass spectrometer (Waters, Milford- MA, USA) [27 ].

Song S., Yin W., Sun X., Cui B., Huang L., Li P., Yang L., Zhou J, & Deng Y. (2020). Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication. The ISME Journal, 14(9), 2248-2260.

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HPLC Purification of Organic Compounds

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Chromatography was achieved over a Phenomenex Gemini-NX semi-prep reversed-phase column (C18, 110 Å, 150 Å~ 10 mm) using an Agilent 1100 series HPLC system and eluting with the following gradient method: 0–2 min 70% B; 2–18 min 70–100% B; 18–20 min 100% B; 20–20.1 min 100–70% B; 20.1–24 min 70% B; flowrate 3.5 mL min⁻¹; injection volume 100 μL; mobile phase A: water + 0.1% formic acid; mobile phase B: acetonitrile. UV absorbance was monitored at 250, 285, 360 & 415 nm.

Devine R., McDonald H.P., Qin Z., Arnold C.J., Noble K., Chandra G., Wilkinson B, & Hutchings M.I. (2021). Re-wiring the regulation of the formicamycin biosynthetic gene cluster to enable the development of promising antibacterial compounds. Cell Chemical Biology, 28(4), 515-523.e5.

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Synthesis of N-(Carboxymethyl)cycloheximide Derivative

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N-(carboxymethyl)cycloheximide (10.0 mg; 29.5 μmol), PyBOP (16.0 mg; 29.5 μmol), and N-ethyldiisopropylamine (10.4 μl; 59.0 μmol) were dissolved in 4 ml DMF and pre-incubated for 5 min. 3-amino-5,7-dimethyl-adamant-1-ol (11.5 mg; 59.0 μmol) was added and the mixture was stirred for 1 h at room temperature. The solvent was evaporated and the crude product was purified via preparative HPLC using a RP C18 column (Phenomenex Gemini-NX, 250 × 21.2) with a linear gradient of 30–80% ACN in 40 min and a flow rate of 17.0 ml/min. The product was achieved with a yield of 6.3 mg (41.4%). [M + H] + calc. = 517.4, [M + H] + found = 517.3.

Rasch J., Theuerkorn M., Ünal C., Heinsohn N., Tran S., Fischer G., Weiwad M, & Steinert M. (2015). Novel Cycloheximide Derivatives Targeting the Moonlighting Protein Mip Exhibit Specific Antimicrobial Activity Against Legionella pneumophila. Frontiers in Bioengineering and Biotechnology, 3, 41.

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Enantioselective Reduction of MT_30.8

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(S)-2-methyl-CBS-oxazaborolidine (1.2 mg; 21.2 μmol) and a 1M solution of BH3 in THF (22.0 μml; 21.2 μmol) were dissolved in 5 ml of anhydrous THF and stirred for 15 min. This solution was added slowly within 30 min to a stirred solution of MT_30.8 (10.0 mg; 21.2 μmol) in 10 ml anhydrous THF at 0°C. The stirring is maintained for 40 min. Thereafter, 10 ml of methanol was added and the solution was evaporated. The crude product was purified via preparative HPLC using a RP C12 column (Phenomenex Gemini-NX, 250 × 21.2) with a linear gradient of 30–80% ACN in 40 min and a flow rate of 17.0 ml/min. The product was achieved with a yield of 5.8 mg (58.0%). [M + H] + calc. = 475.3, [M + H] + found = 475.5.

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Sebocyte Proteome Profiling by iTRAQ

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Protein samples were prepared from sebocytes at D0, D1, D3, D5, and D7 and labeled using iTRAQ Reagent-8 plex multiplex kit (AB SCIEX, Foster City, CA, USA) according to manufacturer's instructions. The iTRAQ-labeled peptides were then subjected to high-pH reversed-phase fractionation in Agilent 1100 HPLC System (Agilent, Palo Alto, CA, USA) equipped with a Gemini-NX (Phenomenex, 00F-4453-E0, Torrance, CA, USA) column (4.6 × 150 mm, 3 μm, 110 Å) as described elsewhere [19 (link), 20 (link)]. The collected fractions were then examined by Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The top ten most intense signals in the acquired MS spectra were selected for further MS/MS analysis. The detected protein threshold was set to achieve a 1% false discovery rate. Original data of mass spectrometry were searched through Mascot 2.2 (Matrix Science Limited, London, UK) under the software platform Proteome Discoverer 2.1 (Thermo Fisher Scientific) against UniProt human protein database (Uniprot-human-160524.fasta) [21 (link), 22 (link)].

Zhang M.F., Cai X.L., Jing K.P., Pi X.X., Liao P.Y., Li S.J., W.e.n.-.L.i., Cai C.C., Quan J.H., Fan Y.M, & G.e.-.S.h.i. (2018). Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes. BioMed Research International, 2018, 7174561.

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Quantifying PTX Loading in SMA-PTX Micelles

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SMA-PTX micelles were solubilized at 1 mg/mL in methanol, and the loading was measured by high-performance liquid chromatography (HPLC) using a BM-20Alite Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan) on a reversed-phase Gemini 3 μm C18, 110A, 150×2 mm column (Phenomenex Gemini-NX) with a 2×4 mm C18 guard column. The mobile phase was acetonitrile and 0.03 M phosphate buffer (pH 3; 1:1 ratio). The flow rate was 1 mL/min with an injection volume of 10 μL, and the column temperature was set at 30°C. PTX peaks were detected at 226 nm with the retention time of 8.5 minutes.

Parayath N.N., Nehoff H., Norton S.E., Highton A.J., Taurin S., Kemp R.A, & Greish K. (2016). Styrene maleic acid-encapsulated paclitaxel micelles: antitumor activity and toxicity studies following oral administration in a murine orthotopic colon cancer model. International Journal of Nanomedicine, 11, 3979-3991.

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Measuring tDodSNO Release from Nanoparticles

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The release of the payloads from the NPs was measured using a semipermeable dialysis membrane (14 kDa molecular weight cutoff, Sigma-Aldrich Co.) as detailed before.15 (link) The concentration of tDodSNO in the solutions was measured using a BM-20Alite Prominence HPLC system (Shimadzu Corporation, Kyoto, Japan) on a reversed-phase Gemini 3 µm C18, 110A, 150×2 mm column (Phenomenex Gemini-NX, North Shore City, Auckland, New Zealand) at 25°C. The mobile phase was methanol containing 0.3% trifluoroacetic acid, and the flow rate was 0.65 mL/min. A diode array detector was set at 341 nm and linked to Chromeleon software for data analysis. tDodSNO peak was detected at 341 nm with the retention time of 4.3 minutes.

Alimoradi H., Greish K., Barzegar-Fallah A., Alshaibani L, & Pittalà V. (2018). Nitric oxide-releasing nanoparticles improve doxorubicin anticancer activity. International Journal of Nanomedicine, 13, 7771-7787.

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Synthesis of N-(carboxymethyl)cycloheximide Conjugate

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N-(carboxymethyl)cycloheximide (10.0 mg; 29.5 μmol), PyBOP (16.0 mg; 29.5 μmol), and N-ethyldiisopropylamine (10.4 μl; 59.0 μmol) were dissolved in 4 ml DMF and pre-incubated for 5 min. 3-Amino-1-adamantol (9.8 mg; 59.0 μmol) was added and the mixture was stirred for 1 h at room temperature. The solvent was evaporated and the crude product was purified via preparative HPLC using a RP C18 column (Phenomenex Gemini-NX, 250 × 21.2) with a linear gradient of 30–80% ACN in 40 min and a flow rate of 17.0 ml/min. The product was achieved with a yield of 7.2 mg (50.0%). [M + H] + calc. = 489.3, [M + H] + found = 489.3.

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Synthesis of Functionalized Naphthyridine Derivative

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Example 169

[Figure (not displayed)]

To a solution of benzyl 6-bromo-4-hydroxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxylate (1.3 g, 3.47 mmol, 1 eq) in DMF (15 mL) was added 4-(2-chloroethyl)morpholine (709.25 mg, 3.81 mmol, 1.1 eq, HCl), Cs₂CO₃(9.03 g, 27.72 mmol, 8 eq) at 20° C., the mixture was stirred at 50° C. for 12 h. The mixture was filtered and the filtrate was purified by prep-HPLC (column: Phenomenex Gemini-NX 80*40 mm*3 μm; mobile phase: [water (10 mM NH₄HCO₃)−ACN]; B %: 10%-30%, 8 min) to give benzyl 6-bromo-4-hydroxy-1-(2-morpholinoethyl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxylate (1.6 g, 3.28 mmol).

LCMS for product (ESI+): m/z 488.0, 490.0 [M+H]⁺, Rt: 0.989 min.

LC/MS (The column used for chromatography was Xbridge Shield RP18 2.1*50 mm, (5 μm particles). Detection methods are diode array (DAD). MS mode was positive electrospray ionization. MS range was 100-1000. Mobile phase A was 10 mM Ammonium bicarbonate in water, and mobile phase B was HPLC grade acetonitrile. The gradient was 5-95% B in 2.05 min 0.5% B in 0.01 mM, 5-95% B (0.01-1.00 min), 95-100% B (1.00-1.80 min), 5% B in 1.81 min with a hold at 5% B for 0.24 min. The flow rate was 1.0 mL/min.

US11564928B1. Cannabinoid receptor type 2 (CB2) modulators and uses thereof (2023-01-31). Teon Therapeutics, Inc. [US]. Inventors: Elfatih Elzein [US], Jiwen Liu [US].

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