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Doxycycline

Manufactured by AK Scientific
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Doxycycline is a broad-spectrum tetracycline antibiotic that inhibits bacterial protein synthesis. It is commonly used in research applications.

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Lab products found in correlation

Azithromycin, by AK Scientific (3 mentions) Ciprofloxacin, by Merck Group (3 mentions) Cefotaxime, by Merck Group (2 mentions) Cefoxitin, by Merck Group (2 mentions) Sulfamethoxazole, by Merck Group (2 mentions) Aztreonam, by AK Scientific (2 mentions) Cefepime, by AK Scientific (2 mentions) Tigecycline, by AK Scientific (2 mentions) Chloramphenicol, by Merck Group (2 mentions) Tetracycline, by Merck Group (2 mentions) Amoxicillin, by Merck Group (2 mentions) Trimethoprim, by Merck Group (2 mentions) Solid phase extraction cartridges 6cc, by Waters Corporation (2 mentions) 4000 qtrap lc ms ms instrument, by AB Sciex (2 mentions) Penicillin g, by Merck Group (2 mentions) Erythromycin, by Merck Group (2 mentions) Clindamycin, by Merck Group (2 mentions) Thioflavin t tht, by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions)

4 protocols using doxycycline

1

Doxycycline and Thioflavin-T Assay

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Doxycycline was purchased from AK Scientific (Union City, CA, USA), and hydrochloric acid was obtained from Merck (Frankfurt, Germany). Sodium azide, thioflavin-T (ThT), deuterium oxide (D2O), deuterated dimethyl sulfoxide (DMSO-d6), DMSO, and all other reagents used for buffer and sample preparation were purchased from Sigma-Aldrich (St. Louis, MO, USA), and were obtained with the highest purity available.
Ferreira E., Almeida Z.L., Cruz P.F., Silva e Sousa M., Veríssimo P, & Brito R.M. (2021). Searching for the Best Transthyretin Aggregation Protocol to Study Amyloid Fibril Disruption. International Journal of Molecular Sciences, 23(1), 391.
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2

Antibiotic Detection in Wastewater: A Comprehensive Approach

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Solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to detect the presence of 16 antibiotics from 7 classes in 11 influent and 11 effluent samples supernatants using a modified protocol 33 (link). Fifty ml of influent or effluent sample were centrifuged at 10,000 rpm for 10 minutes; supernatants were concentrated using solid phase extraction cartridges (6cc, 200 mg, Waters, Milford, MA). Samples were analyzed on a 4000 QTRAP LC/MS/MS instrument (AB Sciex). The data was normalized based on the internal standard (isotopically labeled caffeine, 13C3, Cambridge Isotope Laboratories) to account for experimental variation and antibiotic extraction/ionization efficiency. A mixture of the antibiotic standards was also processed along with the samples as positive controls to test for recovery. Standards for amoxicillin, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, penicillin G, sulfamethoxazole, tetracycline, and trimethoprim were purchased from Sigma-Aldrich (St. Louis, MO). Standards for azithromycin, aztreonam, cefepime, doxycycline, and tigecycline were purchased from AK Scientific (Union City, CA). All analyses were performed at the Proteomics and Mass Spectrometry Facility at the Donald Danforth Plant Science Center (St. Louis, MO).
Pehrsson E.C., Tsukayama P., Patel S., Mejía-Bautista M., Sosa-Soto G., Navarrete K.M., Calderon M., Cabrera L., Hoyos-Arango W., Bertoli M.T., Berg D.E., Gilman R.H, & Dantas G. (2016). Interconnected microbiomes and resistomes in low-income human habitats. Nature, 533, 212-216.
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3

Screening Herbicidal Potential of Antimalarials

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Twenty-eight compounds were tested for an herbicidal effect on A. thaliana. Eight commercially available herbicides were compared to twenty antimalarial compounds (Table 1). Amodiaquine, chloroquine, ciprofloxacin, glufosinate ammonium, glyphosate and oryzalin were obtained from Sigma-Aldrich. Artesunate was obtained from Pharbaco Central Pharmaceutical J.S.C., mefloquine from Roche, and asulam from Sapphire Bioscience. The rest (atovaquone, atrazine, azithromycin, clethodim, clindamycin, cycloguanil, dapsone, dicamba, dihydroartemisinin, doxycycline, halofantrine, lumefantrine, methacycline, piperaquine, primaquine, pyrimethamine, sulfadiazine, sulfadoxine, trifluralin) were sourced from AK Scientific. Stock solutions were prepared in DMSO, except chloroquine and glyphosate, which were prepared in water. Initial screening was at 20 μg/mL and the compounds that showed herbicidal activity were tested at a range of concentrations with DMSO as the negative control and four herbicides as positive controls.
Corral M.G., Leroux J., Stubbs K.A, & Mylne J.S. (2017). Herbicidal properties of antimalarial drugs. Scientific Reports, 7, 45871.
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4

Antibiotic Detection in Wastewater: A Comprehensive Approach

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to detect the presence of 16 antibiotics from 7 classes in 11 influent and 11 effluent samples supernatants using a modified protocol 33 (link). Fifty ml of influent or effluent sample were centrifuged at 10,000 rpm for 10 minutes; supernatants were concentrated using solid phase extraction cartridges (6cc, 200 mg, Waters, Milford, MA). Samples were analyzed on a 4000 QTRAP LC/MS/MS instrument (AB Sciex). The data was normalized based on the internal standard (isotopically labeled caffeine, 13C3, Cambridge Isotope Laboratories) to account for experimental variation and antibiotic extraction/ionization efficiency. A mixture of the antibiotic standards was also processed along with the samples as positive controls to test for recovery. Standards for amoxicillin, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, penicillin G, sulfamethoxazole, tetracycline, and trimethoprim were purchased from Sigma-Aldrich (St. Louis, MO). Standards for azithromycin, aztreonam, cefepime, doxycycline, and tigecycline were purchased from AK Scientific (Union City, CA). All analyses were performed at the Proteomics and Mass Spectrometry Facility at the Donald Danforth Plant Science Center (St. Louis, MO).
Pehrsson E.C., Tsukayama P., Patel S., Mejía-Bautista M., Sosa-Soto G., Navarrete K.M., Calderon M., Cabrera L., Hoyos-Arango W., Bertoli M.T., Berg D.E., Gilman R.H, & Dantas G. (2016). Interconnected microbiomes and resistomes in low-income human habitats. Nature, 533, 212-216.
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