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Mouse monoclonal anti parp 1

Manufactured by Santa Cruz Biotechnology
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Mouse monoclonal anti-PARP-1 is a laboratory reagent designed for the detection of poly(ADP-ribose) polymerase 1 (PARP-1) in various applications. PARP-1 is a nuclear enzyme involved in cellular processes such as DNA repair, genomic stability, and cell death.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) Ab37985, by Abcam (1 mentions) Mab1273, by Cell Signaling Technology (1 mentions) Ab185543, by Abcam (1 mentions) Goat anti rabbit 31460, by Thermo Fisher Scientific (1 mentions) Anti calnexin, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti gm130 antibody, by BD (1 mentions) Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Rabbit polyclonal anti calnexin, by Santa Cruz Biotechnology (1 mentions) Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Sheep anti tgn46, by Bio-Rad (1 mentions) Anti ha rat monoclonal antibody, by Roche (1 mentions) Mouse monoclonal anti v5 antibody, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti flag, by Merck Group (1 mentions) Goat polyclonal anti calnexin, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg conjugated to horseradish peroxidase, by Thermo Fisher Scientific (1 mentions) Cy5 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Cy5 conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions) Cy2 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

PARP-1 (120 citations) Anti-PARP (71 citations) Anti-PARP-1 (65 citations) Mouse anti-PARP (2 citations) Monoclonal mouse anti (2 citations) Mouse monoclonal anti-PARP-1 (F-2, (2 citations)

4 protocols using mouse monoclonal anti parp 1

1

Immunoblotting and Immunofluorescence Protocol

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Antibodies used were mouse monoclonal anti-β-actin (Sigma-Aldrich, A1978; IB 1:50,000), rabbit polyclonal anti-FLAG (Cell Signaling, 2368; IB 1:1,000), mouse monoclonal anti-mitochondrial surface protein p60 (Millipore, MAB1273; IB 1:1,000), rabbit polyclonal anti-VDAC1 (Cell Signaling, 4661; IB 1:1,000), goat polyclonal anti-VDAC2 (Abcam, Ab37985; IB 1:1,000), rabbit polyclonal anti-VDAC3 (Abcam, Ab80452; IB 1:1,000), mouse monoclonal anti-TOM20 (Millipore, Mabt166; IF 1:200), rabbit polyclonal anti-TOM40 (Abcam, Ab185543; IB 1:1,000), rabbit polyclonal anti-HA (Invitrogen, 715500; IB 1:1,000; IF 1:200), rat monoclonal anti-HA (Roche, 12158167001; IB 1:1,000), rabbit monoclonal anti-Bax (Cell Signaling, 5023; IB 1:1,000), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling, 96611; IB 1:1,000), mouse monoclonal anti-PARP-1 (Santa Cruz, sc8007; IB 1:1,000) and rabbit polyclonal anti-calnexin (Santa Cruz, sc11397; IB 1:1,000). Goat anti-mouse (31430; IB 1:5,000), goat anti-rabbit (31460; IB 1:5,000) and donkey anti-goat IgG conjugated to horseradish peroxidase (pa1-28664; IB 1:5,000) were from Thermo Fischer Scientific. Cy™-dye-conjugated donkey anti-mouse and donkey anti-rabbit antibodies (715-225-150, 715-225-152, 715-165-150, 715-165-152, 715-175-150 and 715-175-152; IF 1:250 each) were from Jackson ImmunoResearch Laboratories.
Dadsena S., Bockelmann S., Mina J.G., Hassan D.G., Korneev S., Razzera G., Jahn H., Niekamp P., Müller D., Schneider M., Tafesse F.G., Marrink S.J., Melo M.N, & Holthuis J.C. (2019). Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Nature Communications, 10, 1832.
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2

Antibody Characterization and Validation

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The following antibodies were used: mouse monoclonal anti-β-actin (cat. no. A1978, 1:10,000; Sigma-Aldrich), rabbit polyclonal anti-FLAG (cat. no. 2368, 1:1000; Cell Signaling), mouse monoclonal anti-PARP-1 (sc8007, 1:1000; Santa Cruz), rabbit polyclonal anti-calnexin (sc11397, 1:1000; Santa Cruz), mouse monoclonal anti-V5 antibody (R960-25, 1:4000; Invitrogen), rat monoclonal anti-HA antibody (12158167001, 1:4000; Roche), rabbit polyclonal anti-HA antibody (71-5500, 1:1000 Invitrogen), goat polyclonal anti-calnexin (sc-6465, 1:200; Santa Cruz), sheep anti-TGN46 (AHP500, 1:200 AbD Serotec) and mouse monoclonal anti-GM130 antibody (610823, 1:200; BD Biosciences). Goat anti-mouse (cat. no. 31430, 1:5,000) and goat anti-rabbit IgG conjugated to horseradish peroxidase (31460, 1:5,000) were from Thermo Fischer Scientific. The following secondary antibodies were used for indirect immuno-fluorescence: FITC-conjugated donkey anti-sheep/goat (STAR88F, 1:200; AbD Serotec), Cy3-conjugated donkey anti-mouse IgG (715-165-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-rabbit IgG (711-175-152, 1:200; Jackson ImmunoResearch), Cy2-conjugated donkey anti-mouse IgG (715-225-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-goat IgG (705-175-147, 1:200; Jackson ImmunoResearch), Cy3-conjugated donkey anti-rabbit IgG (711-165-152, 1:200; Jackson ImmunoResearch).
Cabukusta B., Kol M., Kneller L., Hilderink A., Bickert A., Mina J.G., Korneev S, & Holthuis J.C. (2017). ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain. Scientific Reports, 7, 41290.
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3

Lentiviral and Retroviral Transduction Assay in HeLa Cells

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In this analysis, 105 HeLa cells were transduced with LV and RV vectors at different multiplicity of infection. 72 hours, 96 hours, and 120 hours post-transduction cells were harvested, washed twice with phosphate-buffered saline and cells lysates were obtained by using Laemmli buffer (Tris-HCL 20 mmol/l pH7.5, ethylene Diamine Tetraacetic Acid (EDTA) 3 mmol/l, Glycerol 20%, sodium dodecyl sulphate (SDS) 2% and β-Mercaptoethanol 5%). Total protein extracts (20 μg) were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (NUPAGE 4–12% BT GEL 1.0 MM12W, Life Technologies, Monza), transferred to nitrocellulose membranes and probed with mouse monoclonal anti-H2A.X-S139P (cat. 16–193 Merck S.p.A. Milano, Italy), mouse monoclonal anti-CyclinB1 (cat. V152 GeneTex, Irvine, CA), mouse monoclonal anti-p53 (cat. sc-126 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal anti PARP1 (cat. sc-8007 Santa Cruz Biotechnology, Heidelberg, Germany) and antivinculin (cat. V9131, Sigma Aldrich) in Tris-buffered saline with 0.05% Tween (Sigma Aldrich, Milan, Italy) and 5% milk powder (Regilait, Saint Martin Belle Roche France).
Cocchiarella F., Latella M.C., Basile V., Miselli F., Galla M., Imbriano C, & Recchia A. (2016). Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase. Molecular Therapy. Methods & Clinical Development, 3, 16038-.
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4

Protein Expression Evaluation Protocol

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To evaluate the expression of proteins, we used the following antibodies: mouse monoclonal anti-PARP1 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p21 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-MVK (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-pERK (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-ERK1 and anti-ERK2 (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-BIP (1:1000) (Cell Signaling) and mouse monoclonal anti-CHOP (1:100) (Santa Cruz Biotechnology Inc.). Mouse monoclonal anti-β-actin (1:10,000) (Novus Biological) was used as loading control. The goat anti-mouse IgG-Horseradish Peroxidase (Santa Cruz Biotechnology Inc.) and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology Inc.) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS—0.1% Tween20 solution containing 3% of BSA (SERVA).
Romeo M.A., Gilardini Montani M.S., Benedetti R., Garufi A., D’Orazi G, & Cirone M. (2020). PBA Preferentially Impairs Cell Survival of Glioblastomas Carrying mutp53 by Reducing Its Expression Level, Stabilizing wtp53, Downregulating the Mevalonate Kinase and Dysregulating UPR. Biomolecules, 10(4), 586.
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