Anti phospho rps6 ser240 244

Manufactured by Cell Signaling Technology

The anti-phospho-rpS6 (ser240/244) is a lab equipment product that detects the phosphorylation of the ribosomal protein S6 at serine residues 240 and 244. This antibody can be used to monitor the activity of the mTOR signaling pathway.

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Lab products found in correlation

Anti tyrosine hydroxylase, by Merck Group (1 mentions) Anti neun, by Merck Group (1 mentions) Anti chat, by Merck Group (1 mentions) Alexa fluor antibodies, by Jackson ImmunoResearch (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti idh1 r132h, by Dianova (1 mentions) Anti atrx, by Merck Group (1 mentions) Anti egfr, by Agilent Technologies (1 mentions) Anti tp53, by Agilent Technologies (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti rps6, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-RPS6 (28 citations) Phospho-RPS6 (12 citations) Anti-phospho-RPS6 (7 citations)

3 protocols using anti phospho rps6 ser240 244

Immunostaining for Neuronal Markers

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Anti-tyrosine hydroxylase (TH) (1:500; RRID: AB_657012) and anti-ChAT (1:100; RRID: AB_144P) were purchased from Milipore. Anti-phospho-Erk1/2 (1:400; RRID: AB_9101), and anti-phospho-rpS6 (ser240/244; RRID: AB_5364) were from Cell Signaling Technology. Anti-NeuN (1:200; RRID: AB_MAB377) from Chemicon International. Various Alexa Fluor antibodies were used at 1:250 dilutions for immunohistochemistry (Jackson Immuno Research).

Malave L., Zuelke D.R., Uribe-Cano S., Starikov L., Rebholz H., Friedman E., Qin C., Li Q., Bezard E, & Kottmann A.H. (2021). Dopaminergic co-transmission with sonic hedgehog inhibits abnormal involuntary movements in models of Parkinson’s disease and L-Dopa induced dyskinesia. Communications Biology, 4, 1071.

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Protein Expression Analysis of Glioma Biomarkers

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A subset of altered genes and downstream pathways were selected for validation at the protein level. Immunohistochemistry (IHC) was performed at UCSF using a Ventana BenchMark autostainer. Sections were immunostained with commercially available antibodies, including anti-ATRX (Sigma-Aldrich; HPA001906), anti-IDH1 R132H (Dianova; H09), anti-EGFR (Dako; M3563, H11), anti-TP53 (Dako; M7001), anti-RB1 (RB1; BD Biosciences; 554136), anti–phospho-RPS6 (Ser240/244; Cell Signaling Technology; 2215), anti–phospho-AKT1S1 (PRAS40; Thr246; Cell Signaling Technology; 2997, C77D7), and anti–phospho-p44/42 MAPK1/MAPK3 (ERK1/2; Thr202/Tyr204; Cell Signaling Technology; 4370, D13.14.4E). All slides, including positive and negative controls, were reviewed and scored by a neuropathologist (J.J. Phillips).

Byron S.A., Tran N.L., Halperin R.F., Phillips J.J., Kuhn J.G., de Groot J.F., Colman H., Ligon K.L., Wen P.Y., Cloughesy T.F., Mellinghoff I.K., Butowski N.A., Taylor J.W., Clarke J.L., Chang S.M., Berger M.S., Molinaro A.M., Maggiora G.M., Peng S., Nasser S., Liang W.S., Trent J.M., Berens M.E., Carpten J.D., Craig D.W, & Prados M.D. (2017). Prospective Feasibility Trial for Genomics-Informed Treatment in Recurrent and Progressive Glioblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(2), 295-305.

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Antibody Production and Characterization

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The following antibodies were raised in
sheep, by the MRC–PPU Reagents and Services team (https://mrcppureagents.dundee.ac.uk/) and affinity purified against the indicated antigens: anti-SGK3
(S848D, sixth bleed; raised against human SGK3 PX domain comprising
residues 1–130 of SGK3; DU2034), anti-S6K1 (S417B, second bleed;
raised against residues 25–44 of human S6K1: AGVFDIDLDQPEDAGSEDEL),
anti-SGK1 (S062D, third bleed), anti-NDRG1 (S276B third bleed; raised
against full-length human NDRG1; DU1557), anti-Akt1 (S695B, third
bleed; raised against residues 466–480 of human Akt1: RPHFPQFSYSASGTA).
Anti-GAPDH was from Santa-Cruz (sc-32233). Antiphospho-Akt Ser473
(#9271), antiphospho-NDRG1 Thr346 (#5482), antiphospho-TSC2 Ser939
(#3615), anti-TSC2 (#3612), antiphospho-S6K1 Thr389 (#9205), antiphospho-rpS6
Ser240/244 (#2215), and anti-rpS6 (#2217) antibodies were purchased
from Cell Signaling Technology. Total anti-SGK antibody was from Sigma
(#5188).
Secondary antibodies coupled to IRDye680LT or IRDye800CW
were obtained
from Licor Biosciences. Secondary antibodies coupled to horseradish
peroxidase (HRP) were obtained from Thermo Scientific. Secondary antibodies
coupled to Alexa Fluor 488 and Alexa Fluor 594 were obtained from
Thermo Scientific.

Tovell H., Testa A., Zhou H., Shpiro N., Crafter C., Ciulli A, & Alessi D.R. (2019). Design and Characterization of SGK3-PROTAC1, an Isoform Specific SGK3 Kinase PROTAC Degrader. ACS Chemical Biology, 14(9), 2024-2034.

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