HucMSCs from passage 4 were seeded in 12- or 6-well plates and cultured in osteogenic (Cyagen, Santa Clara, USA) and adipogenic differentiation media (Cyagen, Santa Clara, USA) for 21 days. Subsequently, the differentiated cells were fixed in 4% paraformaldehyde (PFA), and adipogenic differentiation was detected by staining lipid droplets with Oil Red O (Cyagen, Santa Clara, USA); mineralization was detected by staining with Alizarin Red (Cyagen, Santa Clara, USA).
Adipogenic differentiation media
Manufactured by Cyagen
Sourced in United States, China
Adipogenic differentiation media is a cell culture medium designed to support the differentiation of stem cells or progenitor cells into adipocytes, the primary cell type found in adipose tissue. This media provides a specific combination of growth factors, hormones, and other nutrients required to induce and maintain the adipogenic differentiation process.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using adipogenic differentiation media
1
Osteogenic and Adipogenic Differentiation of hucMSCs
2
Multilineage Differentiation of Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded onto 24-well plates at a density of 2 × 104 cells/well. For the test group, cells were cultured with osteogenic, chondrogenic and adipogenic differentiation media (Cyagen, China) after reaching 60–70% confluence, respectively. For the control group, cells were cultured with USCs media. After induction for 21 days, cells were stained with Alizarin red S (ARS), Toluidine blue and Oil Red O solution (Cyagen, China) to evaluate the capability of osteogenic, chondrogenic and adipogenic differentiation, respectively.
3
Osteogenic and Adipogenic Differentiation of hDPSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For osteogenic and adipogenic induction, third-passage hDPSCs were cultured in osteogenic and adipogenic differentiation media (Cyagen, USA), respectively. Next, cells were washed twice with phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde for 1 h. After fixation, cells were stained with Alizarin Red or Oil Red O for 30 min. Cells were observed and imaged by an inverted phase-contrast microscope (Olympus, Japan).
4
Adipogenic Differentiation of KSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
P3 KSCs were plated at 104 cells per well into 24-well plates and cultured in adipogenic differentiation media (Cyagen, USA) according to the manufacturer’s instructions. Briefly, solution A was added to the cultured KSCs for three days, and then replaced with solution B for one day. This alternating cycle was repeated three times. KSCs were subcultured in solution B for seven days, stained with oil red O, and adipogenic differentiation was then assessed under an inverted microscope (Olympus, Japan).
5
Visualizing RIPK1's Impact on Adipogenic Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To visualize the effect of RIPK1 on adipogenic differentiation, Oil Red O staining was performed on MSCs. Briefly, after posttransfection, modified MSCs were cultured in adipogenic differentiation media (Cyagen Biosciences, China). Then, cells were fixed, washed, and stained in Oil Red O dye solution (Beyotime Biotech, China) according to the manufacturer's protocol. After incubation for 30 min, the cells were washed with staining buffer and photographed by a microscope [27 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!