Verikine hs mouse ifnβ serum elisa kit

Manufactured by PBL Assay Science

The VeriKine-HS mouse IFNβ serum ELISA kit is a laboratory assay used to detect and quantify the presence of interferon beta (IFNβ) in mouse serum samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) designed to provide accurate and reliable measurements of IFNβ levels.

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Close spelling variants of this product

Verikine-HS mouse IFNβ ELISA kit (2 citations)

5 protocols using verikine hs mouse ifnβ serum elisa kit

Quantifying Cytokine Responses in Human and Murine Cells

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IFNβ levels were determined using assays from PBL Assay Science: VeriKine-HSTM Human Interferon-Beta Serum ELISA Kit (#41415) for human cells’ supernatants or plasma samples, and VeriKine-HS mouse IFNβ serum ELISA kit (#42410-1) for murine plasma samples. Other cytokines (TNF, IL-1β, IL-6, MCP-1α/CCL3, IL-10, IP-10/CXCL10) for human primary cells and plasma samples and 23 cytokines for murine cells (#M60009RDPD; 23-plex Assay) were analyzed using BioPlex cytokine assays from Bio-Rad, in accordance with the instructions of the manufacturer, using the Bio-Plex Pro Reagent Kit III and Bio-Plex 200 System (Bio-Rad). For the analysis of cytokines secretion by THP-1 cells, PMA differentiated THP-1 cells were pretreated with solvents or peptides (12.5 μM) for 30 min followed by 4 h of stimulation with UP K12 LPS (100 ng/ml) and analysis of cytokine expressions in supernatants. All screens were performed in three to five biological replicates for each treatment/peptide. The following ELISA assays were used: human CXCL10/IP-10 DuoSet ELISA (DY266) and human TNF DuoSet ELISA (DY210) from R&D Systems (Biotechne) and human IL-1β ELISA Set II kit (BD OptEIA, BD Biosciences). CyQUANT LDH Cytotoxicity Assay (Thermo Fisher Scientific) was used to measure the extracellular LDH in supernatants as suggested by the manufacturer.

Nilsen K.E., Zhang B., Skjesol A., Ryan L., Vagle H., Bøe M.H., Orning P., Kim H., Bakke S.S., Elamurugan K., Mestvedt I.B., Stenvik J., Husebye H., Lien E., Espevik T, & Yurchenko M. (2023). Peptide derived from SLAMF1 prevents TLR4-mediated inflammation in vitro and in vivo. Life Science Alliance, 6(12), e202302164.

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Quantifying Cytokine Levels in Macrophage Supernatants

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TNF in supernatants was detected using a human TNF-α DuoSet ELISA (DY210-05; R&D Systems), and IFNβ level was detected using VeriKine-HS human IFNβ serum ELISA kit (41415; PBL Assay Science). Supernatants were also analyzed by multiplex cytokine assay (Bio-Plex; Bio-Rad Laboratories) for IL-1β, IL-6, IL-8, and CXCL-10/IP-10. TNF in BMDM supernatants was detected using mouse TNF-α DuoSet ELISA (DY410-05; R&D Systems), and IFNβ level was assessed using a VeriKine-HS mouse IFNβ serum ELISA kit (42410-1; PBL Assay Science). Results are presented as means and SD for biological replicates for representative donors (primary human macrophages) or at least three independent experiments for model cell line THP-1. Statistical significance was evaluated in Prism software. Data distribution was assumed to be normal, but this was not formally tested. The difference between the two groups was determined by a two-tailed t test.

Yurchenko M., Skjesol A., Ryan L., Richard G.M., Kandasamy R.K., Wang N., Terhorst C., Husebye H, & Espevik T. (2018). SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages. The Journal of Cell Biology, 217(4), 1411-1429.

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CSF Collection and IFN-β Measurement

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Cerebrospinal fluid (CSF) was collected, as described (26 (link),27 (link)) with
several adaptions. Anesthetized mice were placed at a 45-degree angle with head
down. After a linear incision at midline above neck, the cisterna magna was
exposed with blunt forceps and dry cotton swab. CSF was collected with a sharp
disposable micropipette (Drummond Scientific Company, Broomall, PA) and
immediately frozen via dry ice and stored (−80°C). Mouse
interferon-β (IFN-β) was measured using Verikine-HS Mouse
IFN-β Serum ELISA Kit (PBL Assay Science, Piscataway, NJ).

Kang K.D., Bernstock J.D., Totsch S.K., Gary S.E., Rocco A., Nan L., Li R., Etminan T., Han X., Beierle E.A., Eisemann T., Wechsler-Reya R.J., Bae S., Whitley R., Yancey Gillespie G., Markert J.M, & Friedman G.K. (2022). Safety and efficacy of intraventricular immunovirotherapy with oncolytic HSV-1 for CNS cancers. Clinical cancer research : an official journal of the American Association for Cancer Research, 28(24), 5419-5430.

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Measuring Cytokine Profiles in Microglia

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Cell culture medium from cultured primary mouse microglia was collected 24 h after stimulation and cleared by centrifugation at 500g for 5 min. Supernatants were diluted 1:10 and assayed using a VeriKine-HS mouse IFNβ serum ELISA kit (PBL Assay Science) according to the manufacturer’s instructions. CXCL10 and CCL5 were measured with a MILLIPLEX MAP mouse cytokine/chemokine magnetic bead kit (Millipore) using a MAGPIX system. For heat map generation, the mean and standard deviation of expression over all samples were calculated for each cytokine, and the expression values were linearly transformed using the formula (concentration – mean) ÷ standard deviation.

Udeochu J.C., Amin S., Huang Y., Fan L., Torres E.R., Carling G.K., Liu B., McGurran H., Coronas-Samano G., Kauwe G., Mousa G.A., Wong M.Y., Ye P., Nagiri R.K., Lo I., Holtzman J., Corona C., Yarahmady A., Gill M.T., Raju R.M., Mok S.A., Gong S., Luo W., Zhao M., Tracy T.E., Ratan R.R., Tsai L.H., Sinha S.C, & Gan L. (2023). Tau activation of microglial cGAS–IFN reduces MEF2C-mediated cognitive resilience. Nature Neuroscience, 26(5), 737-750.

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Quantifying Mouse IFN-β Levels

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The levels of mouse IFN-β protein in culture medium were measured by using the VeriKine-HS Mouse IFN-β Serum ELISA kit (PBL; 42410), following the manufacturer’s protocol.

Baidya S., Nishimoto Y., Sato S., Shimada Y., Sakurai N., Nonaka H., Noguchi K., Kido M., Tadano S., Ishikawa K., Li K., Okubo A., Yamada T., Orba Y., Sasaki M., Sawa H., Miyamoto H., Takada A., Nakamura T, & Takaoka A. (2021). Dual Effect of Organogermanium Compound THGP on RIG-I-Mediated Viral Sensing and Viral Replication during Influenza a Virus Infection. Viruses, 13(9), 1674.

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