Nvp auy922

The human gastric cell lines OE19, OE33, N87, ESO26, and SNU-216 were purchased from the American Type Culture Collection (Manassas, VA, USA) and Korea cell line bank (Seoul, Korea). These cell lines were grown at 37°C in 5% CO₂ in RPMI-1640 and DEME, containing 10% fetal bovine serum from GIBCO (Waltham, MA, USA). NVP-AUY922 and lapatinib were purchased from Selleck Chemicals (Houston, TX, USA), dissolved in DMSO to a final concentration of 10 mmol/L, and stored at −20°C.

TAS‐116 (Taiho Pharmaceutical), 17‐DMAG (Selleck Chemicals), and NVP‐AUY922 (Selleck Chemicals) were dissolved in DMSO and added to the culture medium (1:20 ratio) to adjust for each concentration. The ATL‐related cell lines (Tax‐positive: HuT‐102, OATL4, and KOB; Tax‐negative: ED‐40515, Su9T01, ST1, KK1, SO4, LMY1, and LMY2) and non‐ATL leukemia cell lines (Jurkat, MOLT‐4, and HuT‐78) were maintained in RPMI‐1640 medium supplemented with 10% FCS.¹, ²⁴, ³³ Interleukin‐2 (10 ng/mL) was added in the cultures of IL‐2‐dependent ATL cell lines (KOB, KK1, SO4, LMY1, and LMY2). Peripheral blood samples were collected from nine patients diagnosed with aggressive ATL according to Shimoyama’s diagnostic criteria.⁶ Adult T‐cell leukemia/lymphoma diagnosis was confirmed based on the detection of the monoclonal integration of HTLV‐1 proviral DNA by southern blotting.³⁴ Peripheral blood mononuclear cells were obtained from ATL patients and healthy adult volunteers and isolated using density‐gradient centrifugation on a Lympho‐prep (Axis Shield). CD4⁺ lymphocytes from healthy donors were purified from the PBMCs using the magnetic bead method (CD4⁺ T Cell Isolation Kit; Miltenyi Biotec). The PBMCs were cultured in RPMI‐1640 medium supplemented with 10% FCS and 10 ng/mL IL‐2.