Restriction enzymes were purchased from New England Biolabs (USA). Anti-digoxigenin (DIG) Fab fragment conjugated with horseradish peroxidase (HRP), a DIG DNA labeling kit, and cytotoxic detection kit (LDH) were obtained from Roche (Switzerland). Hoechst 33258, nicotinamide adenine dinucleotide (NAD), poly-D-lysine (PDL), polymyxin B, and 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) were obtained from Sigma-Aldrich (USA). Bradford reagent and γ-globulin standard were purchased from Bio-Rad Laboratories (USA). A limulus amebocyte lysate (LAL) assay kit was obtained from Cambrex Bio Science (USA). Medium, antibiotics and supplementary components for cell culture were purchased from Gibco (USA). Bacterial culture materials were purchased from Becton Dickinson (USA).
2 3 bis 2 methoxy 4 nitro 5 sulfophenyl 2h tetrazolium 5 carboxanilide inner salt xtt
Manufactured by Merck Group
Sourced in United States
2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) is a water-soluble tetrazolium salt. It is used as a reagent in cell-based assays to measure the metabolic activity of cells.
Automatically generated - may contain errors
Lab products found in correlation
Poly d lysine pdl, by Merck Group (1 mentions)
Bradford reagent, by Bio-Rad (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
Polymyxin b, by Merck Group (1 mentions)
Nicotinamide adenine dinucleotide, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Dig dna labeling kit, by Roche (1 mentions)
Cytotoxic detection kit, by Roche (1 mentions)
Fetal bovine serum (fbs), by Cambrex (1 mentions)
Transferrin, by Merck Group (1 mentions)
Interleukin 4 (il 4), by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Dulbecco s modified eagle s medium dmem, by Cambrex (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Thrombin from bovine plasma, by Merck Group (1 mentions)
Hiendo xl endothelial cell expansion medium, by HiMedia (1 mentions)
Trypan blue, by HiMedia (1 mentions)
Trypsin solution, by HiMedia (1 mentions)
4 protocols using 2 3 bis 2 methoxy 4 nitro 5 sulfophenyl 2h tetrazolium 5 carboxanilide inner salt xtt
1
Comprehensive Cytotoxicity Evaluation Protocol
2
Doxorubicin-Transferrin Conjugate Synthesis
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DOX was purchased from Sequoia Research Products (Pangbourne, United Kingdom). Transferrin, 2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) and all reagents for carrying out the conjugation procedure were purchased from Sigma- Aldrich (Darmstadt, Germany). Doxorubicin was bounded to Tf using the modified conjugation procedure developed by Berczi et al. [50 (link)], and specified in Patent Claim No WIPO ST 10/C PL 402896. The conjugate was analyzed using spectrofluorometry, mass spectrometry [51 (link)], and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), according to Lubgan et al. [32 (link)]. TNF-α, TRAIL, IL-6, IL-4, and Human Phospho-Histone H2AX immunoassays were supplied by R&D Systems (Oxford, United Kingdom), whereas the terminal deoxynucleotidyl TUNEL assay was from BioVision (Milpitas, CA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were supplied by Cambrex (Basel, Switzerland). All other chemicals and solvents used in this study were of the highest analytical grade.
3
Endothelial Cell Isolation and Culture
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The L-15 Medium (Leibovitz's), Medium 199, CollagenaseType I, Gelatin solution (2%), Antibiotic-Antimycotic solution (100X), HiEndo XL Endothelial Cell Expansion medium (Reduced serum), Heparin sodium salt, Trypan Blue, Trypsin solution (0.25%) were procured from Himedia. Fetal Bovine Serum (FBS), Poly-l -Lysine, Thrombin from Bovine Plasma and 2, 3 bis (2-methoxy- 4 nitro 5- sulfophenyl) 2H- tetrazolium- 5 carboxanilide inner salt (XTT) were purchased from Sigma (St. Louis, MO,USA). Other chemicals are of analytic grade. Cell culture plastic wares were purchased from Axiva Sichem Pvt. Ltd. (New Delhi, India).
4
Siderophore-Mediated Iron Acquisition Assays
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Pyoverdine (PYOV), 3-hydroxy-1,2-dimethyl-4(1H)pyridine (deferiprone, DFP), celastrol, 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT), and menadione were purchased from Sigma-Aldrich (St. Louis, MO). Amphotericin B (AmB) was derived from X-Gen Pharmaceuticals Inc. (Horseheads, NY). Chrome Azurol S (CAS) was purchased from MP Biomedicals (Solon, OH). Ferri- and desferri-triacetylfusarinine C (TAFC, DF-TAFC) were purified as described previously [32 (link)].
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