GSH, NBT, and catalase from bovine liver (CAT) were purchased from Wako Chemical (Tokyo, Japan). TEMPOL and SOD from human erythrocytes were purchased from Sigma-Aldrich (St. Louis, MO). Other chemicals used in this study were of analytical grade. As the basic solvent of reaction mixtures, 100 mM phosphate buffer (PB) (pH 7.0) containing 0.05 mM diethylenetriaminepentaacetic acid (DTPA) (100 mM PB) was prepared and used for all experiments. Deionized water (Milli-Q system, Merck Millipore, Billerica, MA) was used for preparing 100 mM PB.
Sod from human erythrocytes
Manufactured by Merck Group
The SOD from human erythrocytes is a laboratory product that contains superoxide dismutase, an enzyme naturally found in red blood cells. The core function of this product is to facilitate the dismutation of superoxide radicals into oxygen and hydrogen peroxide, which are less reactive species.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
Tempol, by Merck Group (2 mentions)
Glutathione (gsh), by Fujifilm (2 mentions)
Xanthine oxidase, by Merck Group (2 mentions)
Nadph, by Merck Group (1 mentions)
Hypoxanthine, by Merck Group (1 mentions)
Potassium ferricyanide, by Fujifilm (1 mentions)
N acetyl cysteine (nac), by Fujifilm (1 mentions)
Reduced glutathione gsh, by Fujifilm (1 mentions)
3 protocols using sod from human erythrocytes
1
Antioxidant Enzyme Assay Protocol
2
Antioxidant Assay Protocol
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GSH, Cys, NAC, HCS, Met, and potassium ferricyanide were purchased from Wako Chemical (Tokyo, Japan). TEMPOL, SOD from human erythrocytes, hypoxanthine, xanthine oxidase, NADH, and NADPH were purchased from Sigma-Aldrich (St. Louis, MO). Other chemicals used were of analytical grade. As basic solvents for the reaction mixtures, 100 mM phosphate buffer (PB) containing 0.05 mM DTPA adjusted to pH 7.4 was prepared. Deionized water (deionization by the Milli-Q system) was used to prepare PB.
3
Antioxidant Evaluation Protocol
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4-Hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL), SOD from human erythrocytes, hypoxanthine (HPX), xanthine oxidase (XOD), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO). Reduced glutathione (GSH) was purchased from Wako Chemical (Tokyo, Japan). 5,5-Dimethtyl-1-pyrroline-N-oxide (DMPO) was purchased from LABOTEC Co. (Tokyo, Japan). Other chemicals used in this study were of analytical grade. As the basic solvent of reaction mixtures, 100 mM phosphate buffer (pH 7.0) containing 0.05 mM diethylenetriaminepentaacetic acid (DTPA) (100 mM PB) was prepared and used for all experiments. Deionized water (Milli-Q system, Merck Millipore, Billerica, MA) was used for preparing 100 mM PB.
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