Log In Sign up
The largest database of trusted experimental protocols

Stachrom at 3

Manufactured by Diagnostica Stago
Visit Supplier
Sourced in France

The Stachrom AT III is a laboratory instrument designed to measure the activity of Antithrombin III (AT III) in human plasma samples. AT III is a key regulatory protein involved in the blood coagulation process. The Stachrom AT III provides a quantitative assessment of AT III levels, which is important for diagnosing and monitoring certain coagulation disorders.

Automatically generated - may contain errors

Lab products found in correlation

Stachrom protein c, by Diagnostica Stago (2 mentions) Staclot protein s, by Diagnostica Stago (2 mentions) Stachrom plasminogen, by Diagnostica Stago (1 mentions) Asserachrom d di, by Diagnostica Stago (1 mentions) Sta r evolution, by Diagnostica Stago (1 mentions) Sta r evolution instrument, by Diagnostica Stago (1 mentions) Factor 8 deficient plasma, by Siemens (1 mentions) Sta r analyser, by Diagnostica Stago (1 mentions) Dade thrombin reagent, by Siemens (1 mentions)

5 protocols using stachrom at 3

1

Coagulation Screening for Hereditary Thrombophilia

Check if the same lab product or an alternative is used in the 5 most similar protocols
The coagulation tests used to screen for HT were PC activity (Stachrom® Protein C, Diagnostica Stago, Asnières, France), PS free Ag (Liatest® Free Protein S, Diagnostica Stago), AT activity (Stachrom® AT III, Diagnostica Stago), and plasminogen activity (Stachrom® Plasminogen, Diagnostica Stago), per the international guidelines [19 (link)]. All coagulation tests were performed on the STA®-Evolution Coagulation Analyzer. Reference ranges were determined according to our institutional data. Whenever possible, coagulation tests were repeated 2 weeks after the discontinuation of anticoagulation therapy if results at the time of diagnosis showed low levels of multiple NAs or if tests with abnormal results were performed under anticoagulant use.
Lee S.Y., Kim E.K., Kim M.S., Shin S.H., Chang H., Jang S.Y., Kim H.J, & Kim D.K. (2017). The prevalence and clinical manifestation of hereditary thrombophilia in Korean patients with unprovoked venous thromboembolisms. PLoS ONE, 12(10), e0185785.
+ Open protocol
+ Expand
2

Measurement of Plasma Coagulation Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma D dimer concentration was measured using an anti-human ELISA (Asserachrom® D-Di, Diagnostica Stago, Parsippany, NJ, USA), PAI-1 was measured using an ELISA (Rat PAI-1 Activity ELISA Kit, Catalog #RPAIKT, Molecular Innovations, Novi, MI, USA) configured to detect functional PAI-1 based on its binding to urokinase-coated microtiter plate wells, and antithrombin (AT) was measured using a chromogenic substrate kit (Stachrom® ATIII, Diagnostica Stago, Parsippany, NJ, USA) based on inhibition of bovine thrombin (anti-IIa assay).
Brooks M.B., Turk J.R., Guerrero A., Narayanan P.K., Nolan J.P., Besteman E.G., Wilson D.W., Thomas R.A., Fishman C.E., Thompson K.L., Ellinger-Ziegelbauer H., Pierson J.B., Paulman A., Chiang A.Y, & Schultze A.E. (2017). Non-Lethal Endotoxin Injection: A Rat Model of Hypercoagulability. PLoS ONE, 12(1), e0169976.
+ Open protocol
+ Expand
3

Coagulation Factor Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antithrombin (AT) was measured by chromogenic assay (Stachrom AT III; Diagnostica Stago). Protein C (PC) was measured by clotting assay (Staclot C, Diagnostica Stago). Tissue factor pathway inhibitor (TFPI) was measured using ELISA (Diagnostica Stago).
All factors were expressed as percentages of the mean values in the healthy population. All assays were performed on STA-R Evolution (Diagnostica Stago, Parsipanny, NJ, USA).
Select factor levels (II, V, VII, VIII, IX, X, AT, PC and TFPI) were measured as described above on plasma of the 15 non-Amish controls. FXII and FXI were not measured in this subset as our mathematical model is based on tissue factor (TF) pathway of coagulation and hence measurements of these factors is not needed to run computational models of TF initiated thrombin generation. (Supplement 3, S3, Table S2).
Reference ranges were derived in-house from testing of 123 healthy control subjects and validated with reagent changes.
Gupta S., Bravo M.C., Heiman M., Nakar C., Brummel-Ziedins K., Miller C.H, & Shapiro A. (2019). Mathematical model of thrombin generation and bleeding phenotype in Amish carriers of Factor IX:C deficiency vs. controls. Thrombosis research, 182, 43-50.
+ Open protocol
+ Expand
4

Routine Coagulation Analyses in Citrated Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Routine coagulation analyses were performed in citrated plasma using a STA‐R Evolution® instrument and reagents from Diagnostica Stago (Asniéres, France). STA® SPA+ reagent was used for prothrombin time‐international normalized ratio (PT‐INR), and STA®‐PPT for activated partial thromboplastin time (APTT; Diagnostica Stago) and clot detection methods. The colorimetric kits STACHROM® protein C and STACHROM® ATIII (Diagnostica Stago) were used for analysis of protein C and anti‐thrombin III, respectively. The clot‐based test kit STACLOT® protein S (Diagnostica Stago) and the COATEST® APCTM Resistance V kit from Chromogenix (Bedford, MA, USA) were used for analysis of protein S and activated protein C (APC) resistance, respectively. FVIII, von Willebrand factor antigen and activity, lupus anti‐coagulant, anti‐cardiolipin IgG and IgM and anti‐beta2‐glycoprotein I IgG and IgM were estimated in blood samples from the C5D individual by routine analyses at the Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Landsem A., Fure H., Krey Ludviksen J., Christiansen D., Lau C., Mathisen M., Bergseth G., Nymo S., Lappegård K.T., Woodruff T.M., Espevik T., Mollnes T.E, & Brekke O.‐. (2018). Complement component 5 does not interfere with physiological hemostasis but is essential for Escherichia coli‐induced coagulation accompanied by Toll‐like receptor 4. Clinical and Experimental Immunology, 196(1), 97-110.
+ Open protocol
+ Expand
5

Coagulation Factor Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibrinogen (Dade Thrombin reagent, Siemens, Marburg, Germany), factor VIII coagulant (FVIII:C) (Factor VIII deficient plasma, Siemens), protein S activity (Staclot Protein S, Diagnostica Stago), protein C anticolagulant activity (Cryocheck Clot C, Cryopep, Montpellier, France), and antithrombin (Stachrom AT III, Diagnostica Stago) were measured on a STA-R Analyser (Diagnostica Stago).
Faille D., Lamrani L., Loyau S., Huisse M.G., Bourrienne M.C., Alkhaier S., Cassinat B., Boulaftali Y., Debus J., Jandrot-Perrus M., Chomienne C., Dosquet C, & Ajzenberg N. (2020). Interferon Alpha Therapy Increases Pro-Thrombotic Biomarkers in Patients with Myeloproliferative Neoplasms. Cancers, 12(4), 992.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!