Two hundred and fifty-six Han Chinese patients from Shandong province with RA diagnosed according to the 2008 Classification Criteria of the American College of Rheumatology and 331 healthy controls from the same geographic and ethnic background were included in this study. RA patients were recruited from the department of Rheumatology; Provincial Hospital affiliated to Shandong University, China. Controls had no history of infectious or chronic inflammatory autoimmune diseases and were unrelated to the patients. The study was approved by the Local Ethical Committee of the hospital and university, and informed consent was obtained from all subjects. The methods were carried out in accordance with the approved guidelines. Laboratory parameters were recorded including Rheumatoid factor (RF), anti-cyclic citrullinated peptide antibody (aCCP), anti-Glucose phosphate isomerase (aGPI), erythrocyte sedimentation rate (ESR ) and C-reactive protein (CRP). RF was measured using N Latex RF Kit (SIEMENS, Germany). The level of aCCP and aGPI were determined by enzyme linked immunosorbent assay (ELISA) (AESKULISA, Germany). ESR was determined by the Westergren method via a Monitor-100 machine (BALZELLA, Italy) and CRP by immune turbidimetric analysis (SIEMENS, Germany).
Immune turbidimetric analysis
Manufactured by Siemens
Sourced in Germany
The Immune turbidimetric analysis is a laboratory equipment designed for the quantitative determination of various analytes in biological samples. It utilizes the principle of turbidimetry, where the degree of light scattering caused by the formation of insoluble complexes between the analyte and specific antibodies is measured. This equipment provides a reliable and efficient method for the analysis of proteins, hormones, and other substances in a clinical setting.
Automatically generated - may contain errors
3 protocols using immune turbidimetric analysis
1
Rheumatoid Arthritis Biomarkers in Chinese Patients
2
Cardiometabolic Risk Factors Assessment
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Height and weight were measured, and body mass index (BMI)was calculated as weight (kg) divided by height (m) squared. Also, we measured waist circumference and calculated waist hip ratio. Following overnight fasting, we used a vacuum tube system (Becton-Dickinson) to take a fasting peripheral blood sample (4 mL) from the antecubital vein of each participant and centrifuged the sample at 3000g for 15 minutes to obtain plasma. And the plasma samples were stored in the dark at -80°C. Fasting plasma glucose level was measured by glucose oxidase method (Olympus, Japan). The 2-hour oral glucose tolerance test (OGTT) was performed to assess insulin sensitivity. Next, by using immune turbidimetric analysis (Siemens Healthcare Diagnostics Inc), we measured each participant's plasma hypersensitive C-reactive protein (hs-CRP) and C-reactive protein (CRP). Plasma insulin was measured using a chemiluminescence assay (Beckman Coulter, USA).
3
Cardiometabolic Risk Factors Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Height and weight were measured, and body mass index (BMI)was calculated as weight (kg) divided by height (m) squared. Also, we measured waist circumference and calculated waist hip ratio. Following overnight fasting, we used a vacuum tube system (Becton-Dickinson) to take a fasting peripheral blood sample (4 mL) from the antecubital vein of each participant and centrifuged the sample at 3000g for 15 minutes to obtain plasma. And the plasma samples were stored in the dark at -80°C. Fasting plasma glucose level was measured by glucose oxidase method (Olympus, Japan). The 2-hour oral glucose tolerance test (OGTT) was performed to assess insulin sensitivity. Next, by using immune turbidimetric analysis (Siemens Healthcare Diagnostics Inc), we measured each participant's plasma hypersensitive C-reactive protein (hs-CRP) and C-reactive protein (CRP). Plasma insulin was measured using a chemiluminescence assay (Beckman Coulter, USA).
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