The cells used for immunofluorescence microscopy to detect ATR and phospho-Chk1 foci were processed using essentially the same methods that have been previously described [43 , 44 (link)]. The images were captured using a Carl Zeiss LSM 880 Confocal Laser-Scanning Microscope and processed using ZEN 2.3 software (Carl Zeiss). Anti-phospho-Chk1-S317 (Cell Signaling Technology, 12302; 1:500), anti-ATR (Abcam, ab 2905; 1:500) and anti-γ-H2AX antibodies (Millipore, 05–636; 1:1000) were used.
Ab 2905
Manufactured by Merck Group
The Ab 2905 is a laboratory equipment designed for precise liquid handling. It is a programmable and automated pipetting system that can accurately dispense and aspirate various liquid volumes across multiple samples. The device is engineered to offer reliable and reproducible results for a wide range of scientific applications.
Automatically generated - may contain errors
2 protocols using ab 2905
1
Immunofluorescence Detection of ATR and Chk1 Foci
2
Immunodetection of ATR and Lamin A/C
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Cells were grown and treated with 10 μM MC or DMC on coverslips. Untreated and treated cells were fixed with 4% Formaldehyde and permeabilized with 0.5% Triton-X-100. Immunohistochemistry was done with ATR (Abcam, ab2905) and Lamin A/C (Millipore, MAB3211) antibodies followed by incubation with FITC-conjugated anti-Rabbit (Invitrgen A11037) and Alexa-conjugated anti-mouse (Invitrogen A11058). Coverslips were mounted onto slides using Vectashield mounting medium with DAPI (Fisher Scientific NC9524612, Pittsburgh, PA, USA). Images were collected by PerkinElmer UltraVIEW ERS Spinning Disc Microscope, Waltham, MA, USA. All images were captured using a 100× objective.
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