To examine the morphology of the conidial heads, PDA medium was inoculated with spores and incubated at 37°C for 24 h. The samples were then stained with Fluorescent Brightener 28 (Sigma, USA) and observed using the BX53 fluorescence microscope (Olympus, Japan). Additionally, potato dextrose broth (PDB) medium was inoculated with spores (1 × 105 CFU) and incubated at 37°C for 12 h. The samples were then stained with Fluorescent Brightener 28 and observed using the IX71 fluorescence microscope (Olympus). The samples for transmission electron microscopy (TEM) were prepared following the method described by Weichert et al. (38 (link)). ImageJ was used to measure the thickness of cell wall. The length of three random points on the cell wall was measured, and the average of these values was calculated as a result of cell wall thickness (39 (link)).
Fluorescent brightener 28
Manufactured by Olympus
Fluorescent Brightener 28 is a fluorescent compound used in various laboratory applications. It is designed to enhance the brightness and visibility of materials under ultraviolet (UV) light. The core function of this product is to provide a way to visually identify and analyze substances that exhibit fluorescent properties.
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2 protocols using fluorescent brightener 28
1
Conidial Morphology and Cell Wall Analysis
2
Conidial Morphology and Cell Wall Analysis
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To examine the morphology of the conidial heads, PDA medium was inoculated with spores and incubated at 37°C for 24 h. The samples were then stained with Fluorescent Brightener 28 (Sigma, USA) and observed using the BX53 fluorescence microscope (Olympus, Japan). Additionally, potato dextrose broth (PDB) medium was inoculated with spores (1 × 105 CFU) and incubated at 37°C for 12 h. The samples were then stained with Fluorescent Brightener 28 and observed using the IX71 fluorescence microscope (Olympus). The samples for transmission electron microscopy (TEM) were prepared following the method described by Weichert et al. (38 (link)). ImageJ was used to measure the thickness of cell wall. The length of three random points on the cell wall was measured, and the average of these values was calculated as a result of cell wall thickness (39 (link)).
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