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2 protocols using sodium hydrogen phosphate dibasic

1

Amoxicillin Purification and Characterization

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Amoxicillin reference standard powder was kindly provided by The Accredited Laboratory for Food and Drug Quality Control (Tehran, Iran). Ethanol, sodium hydrogen monobasic, sodium hydrogen phosphate dibasic, sucrose, aluminum chloride, and sodium acetate were supplied from Merck (Dartmouth, Germany). Quercetin reference standard, dextran T10, sodium azide, and dialysis bags (15,000 cutoff) were from Sigma-Aldrich (St Louis, MO, USA).
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2

Oxidative Stress Assays Protocol

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Meta-phosphoric acid, 2-mercaptoethanol, 2,7-dichlorofluorescein diacetate (DCF-DA), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), ethylenediamine-tetraacetic acid (EDTA), GSH, ferric chloride hexahydrate (FeCl3.6H2O), and 5,5-dit hiobis-2-nitrobenzoic acid (DTNB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium and potassium phosphates, saponin, trichloroacetic acid, sodium hydrogen phosphate dibasic, thiobarbituric acid, sodium chloride, sodium citrate, sodium hydrogen phosphate monobasic, n-butanol, methanol, and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were obtained from Merck (Darmstadt, Germany). G6PD activity was determined using a commercial kit (Randox Laboratories, Antrim, United Kingdom). Commercially available Giemsa was purchased from ATR-MED (Tehran, Iran).
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