Gblock hifi gene fragments

Manufactured by New England Biolabs

GBlock HiFi Gene Fragments are synthetic DNA fragments designed for high-fidelity PCR amplification. They are constructed using proprietary technology to ensure accurate and reliable sequences.

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Lab products found in correlation

Hifi dna assembly cloning kit, by New England Biolabs (2 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions) Nc16 pcdna3.1 flag nrf2, by Addgene (1 mentions) Flag tagged wild type amotl2, by OriGene (1 mentions)

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GBlocks (18 citations)

2 protocols using gblock hifi gene fragments

Generating FLAG-tagged NRF Transcription Factors

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The FLAG-NRF2 expression construct was obtained from Addgene (NC16 pCDNA3.1 FLAG NRF2, Plasmid #36971, NM_006164.5), and a site-directed mutagenesis kit (Q5 Site-Directed Mutagenesis Kit, NEB, Ipswich, MA, USA) was used to introduce the T80D mutation. Codon-optimized sequences encoding truncated, FLAG-tagged transgenes of NRF1 (NM_003204.3) and NRF3 (NM_004289.7) were obtained from Integrated DNA Technologies as gBlock HiFi Gene Fragments and cloned into the pCDNA3.1 backbone from FLAG-NRF2 via Gibson assembly using a HiFi DNA Assembly Cloning Kit (NEB).

Ibrahim L., Mesgarzadeh J., Xu I., Powers E.T., Wiseman R.L, & Bollong M.J. (2020). Defining the Functional Targets of Cap‘n’collar Transcription Factors NRF1, NRF2, and NRF3. Antioxidants, 9(10), 1025.

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Cloning of AMOTL2 and MAGI-1 Constructs

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FLAG-tagged wild type AMOTL2 (NM_016201) was obtained from Origene. Codon-optimized sequences encoding truncated, FLAG-tagged transgenes of AMOTL2 and HA-tagged MAGI-1 WW domains were obtained from Integrated DNA Technologies as gBlock HiFi Gene Fragments and cloned into the pCMV6 backbone via Gibson assembly using a HiFi DNA Assembly Cloning Kit (NEB).

Bulos M.L., Grzelak E.M., Li-Ma C., Chen E., Hull M., Johnson K.A, & Bollong M.J. (2023). Pharmacological inhibition of CLK2 activates YAP by promoting alternative splicing of AMOTL2. bioRxiv.

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