Cryopreserved PBMCs were thawed and 1x106 cells per sample washed with PBS containing 2% FCS, 2 mM EDTA, and 0.01% sodium azide at room temperature. After Fc receptor blocking with Gamunex (human immunoglobulin; GRIFOLS) and labeling of dead cells with ethidium monoazide (EMA; Biotinum), the cells were stained with the following titrated monoclonal antibodies: CD3-A700 (clone UCHT1, Biolegend), CD56-FITC (clone HCD56, Biolegend), CD4-PerCP (clone SK3, BD Bioscience), CD8-APC-Cy7 (clone SK1, Biolegend) and PD-1-BV711 (clone EH12.2H7, Biolegend). Lastly, after additional washing the samples were measured with a LSRII cytometer (BD) and processed with BD FACSDiva software 6.1.3. Single color controls were used for automatically calculated compensation.
Cd4 percp clone sk3
Manufactured by BD
CD4-PerCP (clone SK3) is a laboratory reagent used for immunophenotyping. It consists of the monoclonal antibody SK3 conjugated to the fluorescent dye Peridinin Chlorophyll Protein Complex (PerCP). This reagent binds to the CD4 surface antigen expressed on T helper cells, allowing their identification and enumeration through flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Facsdiva software 6, by BD (1 mentions)
Lsrii cytometer, by BD (1 mentions)
Cd56 fitc clone hcd56, by BioLegend (1 mentions)
Pd 1 bv711 clone eh12.2h7, by BioLegend (1 mentions)
Cd8 apc cy7 clone sk1, by BioLegend (1 mentions)
Facslyric cytometer, by BD (1 mentions)
Facsuite clinical software, by BD (1 mentions)
Cd45ra fitc clone l48, by BD (1 mentions)
Cd8 bv421 clone rpa t8, by BD (1 mentions)
Cd3 apc h7 clone sk7, by BD (1 mentions)
2 protocols using cd4 percp clone sk3
1
Phenotyping Immune Cells with Flow Cytometry
2
Flow Cytometric Analysis of α7 Expression
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Cryopreserved peripheral blood mononuclear cells were thawed, washed, and stained with the following fluorophore-conjugated antibodies to determine the level of 7 expression on CD4 + T cells: CD3-APC-H7 (clone SK7, no. 560176, BD Biosciences), CD4-PerCP (clone SK3, no. 347324, BD Biosciences), 7 -PE (clone FIB504, no. 555945, BD Biosciences), CD45RA-FITC (clone L48, no. 347723, BD Biosciences), and CD8-BV421 (clone RPA-T8, no. 562428, BD Biosciences). Data were acquired by BD FACSLyric cytometer using BD FACSuite clinical software (BD Biosciences) and analyzed using FlowJo version 10.1r5.
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