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Cd10 pe cy7

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CD10 PE-Cy7 is a fluorescently-labeled antibody that binds to the CD10 cell surface antigen. CD10 is a cell surface metalloendopeptidase that is expressed on a variety of cell types, including B cells, neutrophils, and certain tumor cells. The PE-Cy7 conjugate provides a bright, photostable fluorescent signal that can be detected using common flow cytometry instrumentation.

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Lab products found in correlation

Cd19 apc cy7, by BioLegend (2 mentions) Facscanto 2 flow cytometer, by BD (2 mentions) Cd20 percp, by BioLegend (2 mentions) Cd21 apc, by BioLegend (2 mentions) Cd27 pe, by BioLegend (2 mentions) Cd19 fitc, by BioLegend (2 mentions) Easysep human cd4 t cell enrichment kit, by STEMCELL (1 mentions) Pd1 pe cy7, by BioLegend (1 mentions) Cd27 percp cy5, by BioLegend (1 mentions) Cd69 pe, by BioLegend (1 mentions) Cd127 percp cy5, by BioLegend (1 mentions) Cxcr5 percp cy5, by BioLegend (1 mentions) Cd3 efluor 605nc, by Thermo Fisher Scientific (1 mentions) Foxp3 alexa 488, by Thermo Fisher Scientific (1 mentions) Tbet pe, by Thermo Fisher Scientific (1 mentions) Cd20 magnetic beads, by Miltenyi Biotec (1 mentions) Cd86 apc, by BioLegend (1 mentions) Icos apc, by BioLegend (1 mentions) Cd4 apc cy7, by BioLegend (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD10-PE (5 citations) Anti-human CD10-PE-Cy7 (3 citations) PE-Cy7-anti-CD10 (2 citations) PE-Cy7 mouse anti-human CD10 (2 citations)

7 protocols using cd10 pe cy7

1

Comprehensive B and T Cell Phenotyping

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B cells were purified from the patient’s peripheral blood by positive selection using CD20 magnetic beads (Miltenyi Biotec, Cambridge, MA). CD4+ T cells were isolated from the peripheral blood of research subjects using the EasySep human CD4+ T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). The following antibodies were used for flow cytometric staining: CD19 APC-Cy7, CD27 PerCP-Cy5.5, CD10 PE-Cy7, CD21 V450, CD69 PE, CD86 APC, FAS Alexa 647, CD4 APC-Cy7, CD127 PerCP-Cy5.5, CD45RO Alexa 700, CXCR5 PerCP-Cy5.5, PD-1 PE-Cy7, ICOS APC (all from Biolegend, San Diego, CA), CD3 eFluor 605NC (from eBioscience, San Diego, CA), and CD21 BD Horizon V450 (BD). Intracellular staining for FOXP3 Alexa 488 (eBioscience) and T-bet PE was performed using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience) in accordance with the manufacturer’s directions.
Minto H., Mensah K.A., Reynolds P.R., Meffre E., Rubtsova K, & Gelfand E.W. (2019). A Novel ATM Mutation Associated with Elevated Atypical Lymphocyte Populations, Hyper-IgM, and Cutaneous Granulomas. Clinical immunology (Orlando, Fla.), 200, 55-63.
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2

Comprehensive Hematopoietic Progenitor Profiling

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Mononuclear cell surface markers were stained with the following fluorochromes: human hematopoietic lineage markers (CD2, CD3, CD14, CD16, CD19, CD56, CD235a) APC (eBioscience), CD34 PE, CD38 Alexa Fluor 700, CD7 PE-Cy5, CD10 PE-Cy7, CD49f PerCP-Cy5.5, CD45RA Brilliant Violet 570, CD135-Biotin, CD127 (IL7Rα) PE-Cy7, SA-APC-Cy7 (BioLegend). Following surface marker staining, cells were fixed with 2% paraformaldehyde, permeabilized with 0.1% Tween-20 and stained with goat anti-human ARID3a antibody (18 (link)), followed by rabbit anti-goat FITC (Invitrogen). Isotype controls (BD Biosciences, eBiosciences, BioLegend) were used for gating hematopoietic progenitor subsets as described (22 (link), 24 (link)) . Doublet exclusion was used to ensure analyses of single cells prior to forward/side scatter gating. Data were collected using an LSRII (BD Biogenics) and FACSDiva (BD Biosciences) software version 4.1, and were analyzed using FlowJo (Tree Star) software version 10.
Ratliff M.L., Ward J.M., Merrill J.T., James J.A, & Webb C.F. (2014). Differential Expression of the Transcription Factor ARID3a in Lupus Patient Hematopoietic Progenitor Cells. Journal of immunology (Baltimore, Md. : 1950), 194(3), 940-949.
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3

Comprehensive Immune Phenotyping of PBMCs

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PBMC were thawed and stained with the LIVE/DEAD Aqua (Life Technologies, Grand Island, NY) viability dye according to the manufacturer protocol. Before fixation, cells were incubated with Human Fc Receptor Binding Inhibitor (eBioscience, San Diego, CA) then stained with the following monoclonal antibodies: CD38-PE-TexasRed (Life Technologies), IgD-FITC (BD Biosciences, San Jose, CA), IgM-Brilliant Violet 421, CD307d (FcRL4)-PE, CD3-Alexa Fluor 700, CD10-PE/Cy7, Streptavidin-APC/Cy7, CD19-PerCP/C5.5 (BioLegend, San Diego, CA), CD24-Biotin, CD21-APC, and CD27-650NC (eBioscience). Cells were then fixed in 2% formaldehyde and analyzed within 1-2 hours on an LSR Fortessa (BD Biosciences). An average of 8×105 events were collected (range of 2×105 to 2×106 events), which resulted in an average of 6.7×104 total B cells analyzed (range of 5×103 to 2×105 B cells). All flow cytometry data was processed using FlowJo Software (Tree Star Inc., San Carlos, CA). Percentages presented for total CD19+ B cell population (CD19+, CD3−) are derived from the percentage of cells within the live gate based on LIVE/DEAD Aqua staining that is within the lymphocyte gate. All B cell subset percentages are the frequency of the total B cell gate (CD19+, CD3−) described above, with the exception of the IgM+ memory B cell subset, which is presented as frequency of CD19+, CD3−, CD10−, CD27+, and IgD−.
Wilmore J.R., Asito A.S., Wei C., Piriou E., Sumba P.O., Sanz I, & Rochford R. (2014). AID expression in peripheral blood of children living in a malaria endemic region is associated with changes in B cell subsets and Epstein-Barr virus. International journal of cancer. Journal international du cancer, 136(6), 1371-1380.
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4

Multiparametric Flow Cytometry Analysis

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Cells were washed in phosphate-buffered saline and incubated for 30 minutes at 4 °C with a combination of monoclonal antibodies against human cell surface antigens as presented in Table 1. Antibodies were purchased as described: CD36 APC, CD14 PE-Cy7, CD42 PE, CD33 PE, CD34 APC, CD45 FITC (all from Becton Dickinson), CD34 Pacific Blue, CD45 Alexa Fluor 700, CD41 APC, CD90 APC, CD10 PE-Cy7, CD19 PE-Cy7 (all from Biolegend, Saint Quentin en Yvelines, France), and CD235a PE, 7-AAD (both from Beckmann Coulter, Villepinte, France). Cells were analyzed on a FACSCanto II flow cytometer (Becton Dickinson) using Diva software (version 6.1.3; Becton Dickinson).
Faivre L., Parietti V., Siñeriz F., Chantepie S., Gilbert-Sirieix M., Albanese P., Larghero J, & Vanneaux V. (2016). In vitro and in vivo evaluation of cord blood hematopoietic stem and progenitor cells amplified with glycosaminoglycan mimetic. Stem Cell Research & Therapy, 7, 3.
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5

Multimodal Characterization of CAR-T Cell Expression

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Surface expression of CD22-CAR-transduced T cells was determined by flow cytometry, using a CD22-Fc chimera (R&D Systems), followed by incubation with phycoerythrin (PE)-F(ab)2 or antigen-presenting cell (APC)-F(ab)2 specific for human immunoglobulin G (IgG)-Fc (Jackson ImmunoResearch Laboratories). Surface expression of CD19-CAR-transduced T cells was detected with anti-CD19 Idiotype or Recombinant Human CD19 Fc Chimera Protein (R&D Systems) conjugated with APC by using the Lightning-Link APC Antibody Labeling Kit (Novus Biologicals). Expression of bivalent CARs was assessed using a combination of both detection reagents as indicated for individual figures. Expression of CD19 and CD22 on B-ALL lines was detected using the following anti-human antibodies: CD45-PerCP-Cy5.5 (eBioscience), CD19-Pacific Blue, CD19-APC-Cy7, CD10-PE-Cy7, and CD22-PE (BioLegend). T cells were characterized with the following antibodies: CD3-APC-Cy7, CD4-Pacific Blue, and CD8a-PE-Cy7 (BioLegend).
Qin H., Ramakrishna S., Nguyen S., Fountaine T.J., Ponduri A., Stetler-Stevenson M., Yuan C.M., Haso W., Shern J.F., Shah N.N, & Fry T.J. (2018). Preclinical Development of Bivalent Chimeric Antigen Receptors Targeting Both CD19 and CD22. Molecular Therapy Oncolytics, 11, 127-137.
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6

B Cell Subset Profiling During Malaria

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PBMCs from during and post-P. vivax infection were used for B cell subset phenotyping. All B cell phenotypic analyses were performed using mouse monoclonal antibodies (mAbs) specific for human B cell markers conjugated to fluorophores as follows: FITC-CD19, PerCP-CD20, PE/Cy7-CD10, PE-CD27, and APC-CD21 (Biolegend). Using this strategy as showed in Supplementary Fig. 4, we observed the proportion of naive B cells as the number of CD10−CD19+CD20+CD21+CD27−cells; plasma cells/blasts were of CD19+CD21−CD20− cells; immature B cells were CD19+CD10+ cells; classical MBCs were CD10−CD19+CD20+CD21+CD27+ cells; atypical MBCs were of CD10−CD19+CD20+CD21−CD27− cells; and activated MBCs were CD10−CD19+CD20+CD21−CD27+ cells. The relative proportions of all the B cell subpopulations as analyzed per total CD19+ B cells for each sample group was determined. FACS analyses were performed on a FACSCanto II flow cytometer (BD Biosciences) using FlowJo software (Tree Star).
Changrob S., McHenry A.M., Nyunt M.H., Sattabongkot J., Han E.T., Adams J.H, & Chootong P. (2018). Persistence of Long-lived Memory B Cells specific to Duffy Binding Protein in individuals exposed to Plasmodium vivax. Scientific Reports, 8, 8347.
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7

B Cell Subsets Profiling in Malaria

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PBMCs collected during acute malaria and 18 months post-infection were used for B cell sub-set phenotyping. Fluorochrome-conjugated, mouse anti-human monoclonal antibodies were used to stain 1 million PBMCs/100 μL FACS buffer. A cocktail consisting of the following mouse monoclonal antibodies was used: FITC-CD19, PerCP-CD20, PE/Cy7-CD10, PE-CD27, and APC-CD21 (Biolegend, San Diego, CA, USA). After staining for 15 min, cells were washed with FACS buffer. Finally, cells were suspended in 250 μL FACS buffer. The analyses were done by a flow cytometer (BD FACSCanto II, Becton–Dickinson Immunocytometry Systems, San Jose, CA, USA). Data were processed using FlowJo software (Tree Star, San Carlos, CA, USA).
Kochayoo P., Changrob S., Wangriatisak K., Lee S.K., Chootong P, & Han E.T. (2019). The persistence of naturally acquired antibodies and memory B cells specific to rhoptry proteins of Plasmodium vivax in patients from areas of low malaria transmission. Malaria Journal, 18, 382.
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