Bond elut c18 cartridge

Manufactured by Agilent Technologies

Sourced in United States

Bond Elut C18 cartridges are solid-phase extraction (SPE) columns designed for sample preparation and purification. They utilize a C18 silica-based sorbent material to selectively retain and concentrate analytes of interest from complex sample matrices.

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Lab products found in correlation

Hypersil gold column, by Thermo Fisher Scientific (3 mentions) Triethylamine tea, by Fujifilm (2 mentions) Xevo tq s, by Waters Corporation (2 mentions) β1 4 galactosyltransferase, by Merck Group (1 mentions) Gemini 5 μm nx c18 reversed phase column, by Phenomenex (1 mentions) Milled lg filter, by Merck Group (1 mentions) 6460 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions) Agilent 1200 separation module, by Agilent Technologies (1 mentions) Masshunter software, by Agilent Technologies (1 mentions) Reacti vap, by Thermo Fisher Scientific (1 mentions) Hplc grade methanol meoh, by Thermo Fisher Scientific (1 mentions) Oasis hlb cartridge, by Waters Corporation (1 mentions) Milli q reagent water system, by Merck Group (1 mentions) Tlc scanner 3, by CAMAG (1 mentions) Hiqsil c18 hs column, by Agilent Technologies (1 mentions) Hplc system model 1100, by Agilent Technologies (1 mentions) Linomat 5, by CAMAG (1 mentions) Tsq vantage triple stage, by Thermo Fisher Scientific (1 mentions) Prominence ultra fast liquid chromatography system, by Shimadzu (1 mentions) Liquid chromatograph mass spectrometer, by Shimadzu (1 mentions)

27 protocols using bond elut c18 cartridge

Synthesis of Sialylated Xylotaxol Derivatives

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Example 9

10-deacetyl-xylosyltaxol (Xyl-taxol) was subjected to galactosylation using bovine milk β-1,4-Galactosyltransferase (Sigma). 2 mM Xyl-taxol (Santa Cruz Biotechnology, 65,34% pure), 40 mM UDP-Gal, 2 mU/μl β-1,4-Galactosyltransferase, 0.22 mM α-lactalbumin and 20 mM MnCl₂in 50 mM MOPS pH 7.2 were incubated in the presence of 5%, 10% or 20% DMSO o/we at +37° C. MALDI-TOF MS analysis of all three reaction mixtures after o/we reactions revealed major signal at m/z 1128 corresponding to β-1,4-galactosylated Xyl-taxol (Gal-Xyl-taxol). The reaction mixtures were purified with Bond Elut C18 cartridge (Varian). Reaction products retained in the cartridge were eluted with 60% aqueous acetonitrile. The Gal-Xyl-taxol product was isolated by HPLC using Gemini 5 μm NX-C18 reversed-phase column (4.6×250 mm (Phenomenex)) eluted with ACN gradient in aqueous ammonium acetate.

The Gal-Xyl-taxol is 9-azido-sialylated by incubation with CMP-9-N₃-sialic acid and P. damsela alpha2,6-sialyltransferase in 0.1 M Tris-HCl, pH 7.5. The 9-azido-sialylated product (Scheme 11) is purified by HPLC on Gemini 5 μm NX-C18 reversed phase column (4.6×250 mm, 110 Å (Phenomenex)) eluted with ACN gradient in aqueous ammonium acetate. The corresponding sialyl derivative is prepared similarly by using CMP-sialic acid instead of CMP-9-N₃-sialic acid in the reaction.

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US10973920B2. Saccharide derivative of a toxic payload and antibody conjugates thereof (2021-04-13). Glykos Finland Oy [FI]. Inventors: Jari Helin [FI], Juhani Saarinen [FI], Tero Satomaa [FI], Filip S. Ekholm [FI].

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Quantitative Analysis of Steroid Hormones

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T, DHT, [16,16,17α-²H₃]-T (T-d₃), and [16,16,17α- ²H₃]-DHT (DHT-d₃) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The Bond Elut C₁₈ cartridge was purchased from Varian (Palo Alto, CA, USA), and 4-dimethylaminopyridine (DAP), 2-methyl-6-nitrobenzoic anhydride (MNBAn), and picolinic acid (PA) were purchased from Tokyo Kasei Industry (Tokyo, Japan). Triethylamine (TEA) was purchased from Wako Pure Chemical Industries (Osaka, Japan). The Cadenza CD C-18 columns and Capcell Pak SCX UG80 pre-columns were purchased from Intact (Kyoto, Japan) and Shiseido (Tokyo, Japan), respectively.
The derivatization reagent was prepared as follows: 10 mg DAP, 20 mg MNBAn, and 25 mg of PA were dissolved in 1 mL of tetrahydrofuran and the mixture was agitated until it became cloudy or crystals appeared. The reagent solution was used after 3–5 min
[5 (link)]. Serum prostate-specific antigen (PSA) levels were measured using a DPC Imrise third generation PSA assay kit.

Miyoshi Y., Uemura H., Umemoto S., Sakamaki K., Morita S., Suzuki K., Shibata Y., Masumori N., Ichikawa T., Mizokami A., Sugimura Y., Nonomura N., Sakai H., Honma S., Harada M, & Kubota Y. (2014). High testosterone levels in prostate tissue obtained by needle biopsy correlate with poor-prognosis factors in prostate cancer patients. BMC Cancer, 14, 717.

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Radiolabeling NOTA-RGD Peptides with F-18

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The ¹⁸F labeling of NOTA coupled RGD
peptides followed a previously published procedure with some modifications.^{1 (link)} Briefly, 3 μL of 2 mM aluminum chloride
(6 nmol) in 0.5 M pH 4 sodium acetate buffer was added to a 1 mL polypropylene
tube containing 6 μL of 2 mM NOTA coupled RGD peptide (12 nmol).
Then, 0.15 mL of acetonitrile and 0.05 mL of aqueous [¹⁸F]fluoride (0.37–0.74 GBq) were added to the vial. The vial
was sealed and heated at 100 °C for 10 min to form the radioactive
aluminum-fluoride NOTA complex. The vial was cooled and diluted with
10 mL of water and trapped on a Varian Bond Elut C₁₈ cartridge
(100 mg). The radioactivity trapped on the C₁₈ cartridge
was eluted with 0.3 mL of ethanol containing 1 mM HCl, the ethanol
solution was evaporated with argon flow, and the final product was
dissolved in PBS. The radioactive products were analyzed with radio
HPLC before and after evaporating the ethanol.

Lang L., Ma Y., Kiesewetter D.O, & Chen X. (2014). Stability Analysis of Glutamic Acid Linked Peptides Coupled to NOTA through Different Chemical Linkages. Molecular Pharmaceutics, 11(11), 3867-3874.

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Bile Acids Extraction and Profiling

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The BAs in feces were extracted according to the methods described previously (Fang et al., 2018 (link)). Briefly, 50 to 80 mg of lyophilized feces was added into a mixture of pre-cold sodium acetate buffer (50 mM, pH 5.6) and ethanol (chromatography grade), followed by 2-min mixing and 20-min centrifugation at 20,000 g. The supernatant was diluted five times with a sodium acetate buffer and applied to a Bond Elut C18 cartridge (Varian, Palo Alto, CA, United States), which was then washed with 25% ethanol. BAs were eluted with 5 ml methanol. After the solvent was evaporated with nitrogen gas, the residue was dissolved in 1 ml of methanol and then passed through a 0.45-μm Milled-LG filter (Millipore, Billerica, MA, United States) to get the filtrate for BA analyses. A Waters Xevo TQ-S LC/MS mass spectrometer (Waters, Milford, MA, United States) equipped with an ESI source was adopted to determine the BA profile in samples, and all the assay conditions were in accordance with a previous study (Fang et al., 2018 (link)). The concentration of each BA in samples was determined based on the series dilutions of standards, and good linearity was confirmed. In this study, a total of 18 BA standards were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Yan H., Wei W., Hu L., Zhang Y., Zhang H, & Liu J. (2021). Reduced Feeding Frequency Improves Feed Efficiency Associated With Altered Fecal Microbiota and Bile Acid Composition in Pigs. Frontiers in Microbiology, 12, 761210.

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Radiolabeling of NEB with 64Cu

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To a 1-mL plastic tube containing 11.0 μg of NEB in 100 μL of 0.4 M, pH 5.5, sodium acetate buffer was added 5 μL of aqueous ⁶⁴Cu-CuCl₂ solution (262.7 MBq). The mixture was stirred in a vortex mixer and heated on an 80°C heating block for 10 min. The tube was cooled and the radioactive solution transferred to a 10-mL syringe containing 10 mL of water. This solution was passed through a Varian Bond Elut C₁₈ cartridge (100 mg), and the desired product was trapped on the cartridge. The radioactivity trapped on the C₁₈ column was eluted with 0.45 mL of 80% ethanol/water with 1 mM HCl to give 185 MBq of the desired product in a 70% radiochemical yield. The ethanol solution was evaporated with argon flow, and the final product was dissolved in phosphate-buffered saline and analyzed by HPLC. The radiochemical purity was greater than 95%.

Niu G., Lang L., Kiesewetter D.O., Ma Y., Sun Z., Guo N., Guo J., Wu C, & Chen X. (2014). In Vivo Labeling of Serum Albumin for PET. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 55(7), 1150-1156.

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Synthesis and Purification of Deuterated Steroid Hormones

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T, DHT, [16,16,17α-2 H3]-T (T-d3), [16,16,17α-2 H3]-DHT (DHT-d3), and 5-butylpicolinic acid (BuPi) were purchased from Sigma-Aldrich (St. Louis, MO, USA). In addition, 4-dimethylaminopyridine (DMAP), 2-methyl-6-nitrobenzoic anhydride (MNBAn), picolinic acid (Pi), 3-methylpicolinic acid (3-MePi), and 6-methylpicolinic acid (6-MePi) were obtained from Tokyo Chemical Industry (Tokyo, Japan) and triethylamine (TEA) was purchased from Wako Pure Chemical Industries (Osaka, Japan). A Bond Elut C18 cartridge was obtained from Varian Inc. (Lake Forest, CA, USA).

Ohno K.I., Hasegawa T., Tamura T., Utsumi H, & Yamashita K. (2018). Proton Affinitive Derivatization for Highly Sensitive Determination of Testosterone and Dihydrotestosterone in Saliva Samples by LC-ESI-MS/MS. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 34(9).

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Serum Bile Acid Profiling using LC-MS/MS

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Serum BA profiles were detected using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system, as previously described.^{(17 (link),38 (link))} Briefly, 20 µl of mouse serum was diluted 100-fold with ²H-labelled internal standards and 0.5 M potassium phosphate buffer (pH 7.4). The mixture was injected into a Bond Elut C18 cartridge (200 mg; Agilent Technologies, Santa Clara, CA). Target molecules were eluted in water/ethanol (1:9, vol/vol). The eluate was evaporated under nitrogen until dry and then dissolved in 20 mM ammonium acetate buffer (pH 7.5)/methanol (1:1, vol/vol). An aliquot of the resulting sample solution was injected into the LC-MS/MS system for analysis. Chromatographic separation was performed using a Hypersil GOLD column (150 × 2.1 mm, 3 µm; Thermo Fisher Scientific, Waltham, MA). A mixture of 20 mM ammonium acetate buffer (pH 7.5), acetonitrile, and methanol (70:15:15, vol/vol/vol) was used for the initial mobile phase and was gradually changed to 30:35:35 (vol/vol/vol) over 30 min.

Ushiroda C., Naito Y., Takagi T., Uchiyama K., Mizushima K., Higashimura Y., Yasukawa Z., Okubo T., Inoue R., Honda A., Matsuzaki Y, & Itoh Y. (2019). Green tea polyphenol (epigallocatechin-3-gallate) improves gut dysbiosis and serum bile acids dysregulation in high-fat diet-fed mice. Journal of Clinical Biochemistry and Nutrition, 65(1), 34-46.

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Quantitative Analysis of Plant Hormones

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Root samples were harvested 1–12 mm from the root–shoot junction at 0, 2 and 8 h after root cutting, and frozen in liquid nitrogen. The hormone analysis was carried out as described previously (Miyamoto et al. 2016 (link), Enomoto et al. 2017 (link)). Briefly, samples of approximately 100 mg FW were suspended in 80% (v/v) aqueous methanol with [¹³C₆]IAA, [²H₂]JA and [¹³C₆]JA-Ile as internal standards. Samples were homogenized and the supernatant was loaded onto a Bond Elut C18 cartridge (100 mg, 3 ml; Agilent Technologies) and eluted with 80% (v/v) aqueous methanol. The concentrated samples were subjected to liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) composed of a quadrupole tandem mass spectrometer (Agilent 6460 Triple Quadrupole mass spectrometer) with an electrospray ion source and an Agilent 1200 separation module. The raw data were extracted from the MassHunter software (Agilent Technologies) and examined in Excel (Microsoft).

Xu D., Miao J., Yumoto E., Yokota T., Asahina M, & Watahiki M. (2017). YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting. Plant and Cell Physiology, 58(10), 1710-1723.

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Solid-Phase Extraction of Compound Library

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The library (60 mg) was dissolved in 6.0 mL of 5% MeCN in water with 0.1% TFA. 6 mL Bond Elut C18 cartridge (Agilent, P/N 12256130) was used for the solid phase extraction. A cartridge was conditioned with MeCN with 0.1% TFA (~10 mL), and then equilibrated with 1% MeCN in water with 0.1% TFA (~10 mL). Afterward, the library solution was loaded, and the cartridge was washed with 1% MeCN in water with 0.1% TFA (~10 mL). Sample elution was achieved by passing 70% MeCN in water with 0.1% TFA (~10 mL) through the cartridge. The final eluate was collected separately and lyophilized.

Ye X., Lee Y.C., Gates Z.P., Ling Y., Mortensen J.C., Yang F.S., Lin Y.S, & Pentelute B.L. (2022). Binary combinatorial scanning reveals potent poly-alanine-substituted inhibitors of protein-protein interactions. Communications Chemistry, 5, 128.

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Plasma BA Profiling via LC-MS/MS

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Plasma BA profiles were detected using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system.⁶⁷ (link) Briefly, mouse plasma (20 µL) was diluted 100-fold with ²H-labeled internal standards and 0.5 M potassium phosphate buffer (pH 7.4). The mixture was applied to a Bond Elut C18 cartridge (200 mg; Agilent Technologies, Santa Clara, CA, USA). The target molecules were eluted in water/ethanol (1:9, v/v). The eluate was evaporated under nitrogen until dry and dissolved in 20 mM ammonium acetate buffer (pH 7.5)/methanol (1:1, v/v). An aliquot of each sample was injected into the LC-MS/MS system for analysis. Chromatographic separation was performed using a Hypersil GOLD column (Thermo Fisher Scientific, Waltham, MA). A mixture of 20 mM ammonium acetate buffer (pH 7.5), acetonitrile, and methanol (70:15:15, v/v) was used for the initial mobile phase, which was gradually changed to 30:35:35 (v/v/v) over 30 min.

Aoi W., Inoue R., Mizushima K., Honda A., Björnholm M., Takagi T, & Naito Y. (2023). Exercise-acclimated microbiota improves skeletal muscle metabolism via circulating bile acid deconjugation. iScience, 26(3), 106251.

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