Hyperion 1000

The morphology and shape of HSQ microstructures constructed by FsLDW were measured by using field-emission scanning electron microscope (FE-SEM, S-4800, Hitachi) at 5 kV accelerating voltage after being deposited with a thin layer of Au. The surface roughness of the HSQ microstructures were measured using an atomic force microscope (AFM, Fastscan IconBio, Bruker). The optical microscopy images were characterized by a laser scanning confocal microscope (LSCM, A1R MP, Nikon) using an oil-immersion objective lens (N.A. = 1.40, 60 ×, Nikon). Raman and FT-IR spectra of HSQ film with and without fs laser exposure were characterized by using Raman spectroscopy (Raman-11, Nanophoton, excitation wavelength is 532 nm) and FT-IR spectroscopy (Vertex 70, Bruker) equipped with microscope (Hyperion 1000, Bruker). The reflectance spectra of the structural colour before and after thermal treatment were measured by using FT-IR spectroscopy (Vertex 70, Bruker) equipped with microscope (Hyperion 1000, Bruker). Structural colour of the HSQ microstructure was recorded by employing a charge coupled device (CCD) camera (Beijing Groupca Company) equipped on an optical microscope under reflection mode. The absorption spectrum of the HSQ film on quartz was recorded on a UV-Visible Spectrophotometer (UV-2550, Shimadzu).

TP, β-CD and the TP-β-CD inclusion complex were obtained separately with IR grade potassium bromide at a ratio of 1:100, and IR pellets were prepared by applying 8 metric tonnes of pressure in a hydraulic press. The vibrational infrared spectra were measured between 500 and 4000 cm^-1, with an FT-IR Bruker Equinox 55 spectrometer equipped with a Bruker Hyperion 1000 microscope. In order to analyze changes in the positions and intensity of bands in experimental spectra of the TP-β-CD inclusion complex, quantum-chemical calculations were performed based onB3LYP functional and 6-31G(d,p) as a basis set. All calculations were performed using the Gaussian 09 package and the GaussView application [21 ].

RU, β-CD andRU-β-CD inclusion complex co-grind mixture were obtained separately with IR grade KBr in the ratio of 1: 100, and corresponding pellets were prepared by applying 8 metric ton of pressure in hydraulic press. The vibrational infrared spectra were recorded between 400 and 5000 cm^-1, with an FT-IR Bruker Equinox 55 spectrometer equipped with a Bruker Hyperion 1000 microscope. In order to analyse changes in positions and intensity in experimental spectra of RU-β-CD inclusion complex, quantum chemical calculations based on DFT were performed. All the calculations were made by using the Gaussian 03 package [18 ]. The GaussView application was utilized to propose the initial geometry of the investigated molecules and to visually inspect the normal modes.