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Hb 301

Manufactured by HemoCue
Sourced in Sweden, United States

The HemoCue Hb 301 is a portable, easy-to-use device designed to measure hemoglobin levels in blood samples. It provides quick and accurate results, enabling healthcare professionals to make timely decisions regarding patient care.

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43 protocols using hb 301

1

Capillary Blood Hb Measurement Using HemoCue

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After venous blood collection, a capillary puncture of the middle or ring finger (children ≥1 year) or heel (children <1 year) was performed using disposable lancets. Three samples of capillary blood were collected from each child. The first and second sample tests (sample test 1 and sample test 2) were collected from the same puncture for the analysis for HemoCue® reproducibility. The third sample test (sample test 3) was obtained from a second puncture performed on another finger or on another region of the heel in order to evaluate method reproducibility. According to the portable haemoglobinometer manufacturer recommendations, the first drop was discarded for each puncture.
Hb concentration in capillary blood samples was carried out by a portable HemoCue® Hb 301 model haemoglobinometer by the azide methaemoglobin detection method (test method)(13 (link)).
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2

Evaluating Malaria Interventions in Villages

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A survey was conducted in each village at the end of the transmission season, in December—1 month after the last SMC cycle—to take a finger prick blood sample to make blood films for measurement of Plasmodium falciparum parasitaemia and gametocyte carriage, to measure Hb concentration using a hemocue machine (Hb301, HemoCue, Ängelholm, Sweden), and to take blood spots on filter paper for molecular analysis of markers of resistance to SMC drugs. This survey included 35 children in each age group (aged less than 5 years and 5–9 years) in each village, by simple random sampling from the census list. A questionnaire was administered to assess use of insecticide-treated bednets by study children; caregivers’ knowledge, attitudes, and practices in relation to malaria; acceptability of SMC; and adherence to the unsupervised AQ doses in the last SMC cycle.
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3

Comprehensive Micronutrient and Parasite Assessment

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Blood samples (4mls) will be drawn at baseline and endline by trained nurses who are skilled and experienced in taking paediatric blood samples. One to two drops will be used immediately to measure haemoglobin (using a HemoCue HB301 photometer). Of the remaining blood, 2mls will be placed in a trace element sodium heparin vacuette for further micronutrient analysis. The remaining blood will be placed in Ethylenediamine tetraacetic acid (EDTA) tubes for fatty acid analysis, then 40 μl will be pipetted onto pre-treated chromatography paper to be analysed as a dried blood spot. Blood samples will be stored, transported and analysed appropriately to avoid contamination and deterioration. Analysis will be conducted for micronutrient status including haemoglobin (g/l), ferritin (μg/l), transferrin receptor (mg/l), retinol-binding protein (vitamin A status) (μmol/l), zinc (μmol/l), C reactive protein (mg/l), using internationally accepted indicators [46 ].
Stool samples will be taken and tested for parasites. Stool containers will be distributed to caregivers and collected the following day. Analysis will be conducted using FLOTAX method.
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4

Hemoglobin Measurement and Anemia Assessment

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A single measurement of hemoglobin concentration in each child was conducted using a portable hemoglobin analyzing system HB 301+ (HemoCue® AB, Angelhome, Sweden). Classification of anemic or non-anemic children, and subsequently in mild, moderate and severe anemia, was performed according to the reference values adjusted for the altitude [17 ] (Table 1) as Lubango is 1786 meters above sea level [18 ].
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5

Hemoglobin Screening for Anemia

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Haemoglobin concentration was determined using a portable photometer (Hemocue HB 301; Ängelholm) from a blood sample from fingerpicks. The haemoglobin readings were adjusted for altitude (average 1900 m above sea level) and were categorized into nonanaemic and anaemic (any anaemia). Women with adjusted haemoglobin concentrations <12 g/dl were considered to be anaemic (WHO, 2011 ) and were referred to local health centres. Screening for malaria was performed using a rapid diagnostic test.
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6

Anemia Assessment Among Pregnant Women

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Data were collected using pretested interviewer administered questionnaire, which contains sociodemographic characteristics (age, education, occupation, marital status, and others), obstetric and gynecological history (trimester, gravidity, parity, ANC follow up, iron supplementation, and others), and dietary factors (type of stable diet, meal frequency, and intake of meat, tea, egg, and milk and milk products).
Blood hemoglobin concentration was measured using a HemoCue Hb 301, a precalibrated instrument designed for the measurement of hemoglobin concentration. Venous blood was drawn, through microcuvettes, and inserted into the HemoCue and the result was recorded. Mid upper arm circumference (MUAC) of the mothers was measured and recorded as an independent variable.
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7

Hemoglobin Analysis in Pregnant Women

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Hemoglobin analysis was performed at the station. The hemoglobin concentration was measured by taking one finger-prick blood sample from each pregnant woman using a HemoCue Hb 301. A prick was made on the tip of the middle finger after the site was cleaned with a disinfectant. The first drop of blood was cleaned, and the second drop was collected to fill the microcuvette which is then placed in the tank holder of the hemoglobin concentration measuring device. The performance of the meter was verified daily using a control standard to increase the reliability of the test. First, the microcuvette holder was pulled to its loading position and when the reader was ready to use capillary blood, the sample to be examined was filled in one continuous process. Within 10 min of filling, it was placed in the holder and pushed into its measuring position. Finally, after 15 to 60 sec, the result was displayed and recorded using WHO field survey recommendation techniques (9 (link)).
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8

Evaluation of AL and ASAQ for Pediatric Malaria

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Children were treated with either AL (Cipla, Mumbai, India) or ASAQ (Sanofi-Aventis, Maphar, Morocco) for 3 days. The dosage was weight based according to the manufacturers’ recommendations. All ASAQ doses were directly observed by study staff and were administered with water or juice. For AL, morning doses were observed by study staff and were administered with yogurt or milk. Parents or guardians were given the evening dose and a yogurt or milk packet to give at home. Study staff telephoned parents or guardians in the evening to remind them to administer the evening dose, and compliance was further assessed by requesting parents or guardians to bring empty blister packets to the clinic the following day.
Children were monitored daily for the first 4 days and then weekly thereafter for a total of 28 days. At each visit, study staff performed clinical exams, performed blood smears (except for day 1), and collected blood on Whatman 903 filter paper (GE Healthcare Life Sciences, Marlborough, MA, USA). Hemoglobin was assessed fortnightly using HemoCue Hb301 (Hemocue, Ängelholm, Sweden) or DiaSpect (EKF Diagnostics, Barleben, Germany) hemoglobinometers.
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9

Cardiovascular Risk Factors and Health Measures

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The following variables, which were measured using a questionnaire, were selected as covariates: age (years; continuous), sex (male/female), educational attainment (none/primary/secondary/tertiary), household wealth index derived as a principal component analysis of properties belonging to household of the participant [34 (link)] and study area. We further considered CVD risk factors including smoking status (never/former/current), intake of fruits and vegetables (never/monthly/weekly/several days per week), sedentariness (hours per week spent sitting or lying excluding sleep; continuous), family history of hypertension (yes/no) and general susceptibility to illness (in comparison to same sex and age group; lower/higher). Participants also reported fever (yes/no) before examination.
Weight (kg), height (m) and waist circumference (cm) were measured and BMI was calculated as the weight–height-squared ratio (kg/m2). Body temperature was measured using Omron auricular thermometer (Omron Healthcare, Kyoto, Japan) and was classified along the mean (≤36.5 °C/>36.5 °C; range: 34.6–37.9 °C) for analyses. Hb was measured with HemoCue Hb 301 (HemoCue AB, Ängelholm, Sweden), and anaemia was defined based on WHO recommendations [38 ].
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10

Portable Hemoglobin Measurement Protocol

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About 10 μl of venous blood was used to estimate haemoglobin concentration using a portable haemoglobinometer, which gives readings in g/dl, according to manufacturer’s instructions (HemoCue Hb 301, HemoCue®, Sweden). A new standard cuvette supplied together with the haemoglobinometer was used every day for quality control. Haemoglobin concentrations were expressed in g/dl and the results were recorded and categorised into two: normal (≥11.5 g/dl of blood) or anaemia (<11.5 g/dl of blood) [30 ].
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