Individually housed rats were habituated to 1 h access of high fat diet (SF00-219, Specialty Feeds, Western Australia) in the light cycle for 4 days. During test day, chow was removed 2 h prior to test. High fat diet was presented during mid-light cycle and the amount consumed within 1 h was recorded, after which high fat diet was replaced with chow.
Sf00 219
Manufactured by Specialty Feeds
Sourced in Australia
The SF00-219 is a laboratory equipment product designed for specific functions in a research or testing environment. It is a compact and versatile unit that can be utilized in various applications. The core function of the SF00-219 is to perform precise measurements and analyses as required by the user's needs. No further details about the intended use or specific applications of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Standard ain93g rodent diet, by Specialty Feeds (1 mentions)
Am1241, by Cayman Chemical (1 mentions)
Am630, by Cayman Chemical (1 mentions)
High fat diet (hfd), by Specialty Feeds (1 mentions)
One touch glucometer, by Roche (1 mentions)
Xylazine, by Troy Laboratories (1 mentions)
Neutral buffered formalin, by Merck Group (1 mentions)
Vacutainer blood collection tube, by BD (1 mentions)
Am251, by Cayman Chemical (1 mentions)
Isoflurane inhalation, by Abbott (1 mentions)
Ain93g, by Specialty Feeds (1 mentions)
14 protocols using sf00 219
1
Rat Feeding Behavior and High-Fat Diet
2
Dietary Manipulation in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Upon delivery, all animals were allowed to acclimatize for at least 1 week before commencement of dietary manipulation. Rats were randomly assigned to either a control diet (CON, Standard AIN93G rodent diet, 6% total fat including 1.05% total saturated fatty acids; Specialty Feeds, Perth, Australia) or WD (SF00-219, 21% total fat including 1.80% total saturated fats and 0.15% cholesterol; Specialty Feeds, Perth, Australia) and remained on this diet for 12 weeks.
3
BCG Vaccination in ApoE-/- Mice on Western Diet
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethical approval was obtained for all animal experimentation from the relevant Monash University Animal Ethics Committee (AEC). ApoE−/− mice on a C57Bl/6/J background were bred and held in the Monash Medical Centre (MMC) Specific Pathogen Free (SPF) animal facility until termination of the experiment at 16 weeks. At 2 days of age, subcutaneous BCG was administered as described below. At 4 weeks, pups were weaned and fed a high (22%) fat, 0.15% cholesterol ‘Western fast food diet’ (Specialty Feeds, Australia; SF00-219). At 16 weeks (mature adulthood), mice were transferred to the conventional animal facility at MMC for tissue collection (see Figure 1 A for summary timeline).
Investigators administering BCG vaccine or saline injections were blinded to treatment. Immunohistochemical staining was done in single batches for each antigen to minimize variation. Each histological variable was measured by an individual investigator who was blinded to treatment.
Investigators administering BCG vaccine or saline injections were blinded to treatment. Immunohistochemical staining was done in single batches for each antigen to minimize variation. Each histological variable was measured by an individual investigator who was blinded to treatment.
4
Atherosclerosis Regression in Ldlr-/- Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To perform the atherosclerotic regression experiments, female Ldlr−/− mice were fed a western‐type diet (WTD; 22% Fat, 0.15% cholesterol; catalogue #SF00‐219, Specialty Feeds) for 14 weeks. To induce lesion regression, Ldlr−/− were then switched to a normal chow diet for 3 weeks. Mice undergoing regression were divided into three groups; (1) control, (2) K/BxN serum transfer arthritis (2× intraperitoneal injection of K/BxN serum; 100 μL per injection) and received vehicle (30% DMSO‐saline, I.P.) or (3) rendered arthritic using K/BxN serum and were administered LXR agonist T0901317 daily for 2 weeks (25 mg kg−1, I.P.).
5
High-Fat Diet and Cannabinoid Receptor Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the acclimatization period, rats received a high-fat diet (HFD) 21% fat content (equating to 40% digestible energy) from lipids (Specialty Feeds SF00-219, Glen Forrest, WA, Australia) for 9 weeks, as described in our previously published study [13 (link)]. Throughout the study, animals could access food and water ad libitum. Animals were then maintained on the HFD and treated for a further six weeks with a daily i.p. injection, with either vehicle (0.9% isotonic saline solution containing 0.75% Tween 80: n = 9–10), 3 mg/kg body weight of AM1241 (Cayman Chemicals, Ann Arbour, MI, USA), or 0.3 mg/kg body weight of AM630 (Cayman Chemicals, Ann Arbour, MI, USA) dissolved in the vehicle solution (n = 9–10). These compounds and their doses were chosen at the time of the initiation of the study due to the following papers: AM1241 [32 (link)] and AM630 [31 (link)]. EchoMRI Whole-Body Composition Analyzer (EchoMRI-900; EchoMRI, Houston, TX, USA) was used to determine body composition, as previously described [6 (link)].
Following treatment, rats were anesthetized with 3% isoflurane inhalation (Abbott Laboratories, Chicago, IL, USA) with cardiac blood collected to confirm their death; then, all other major organs including fat pads were removed post-mortem, weighed, snap frozen in liquid nitrogen, and stored at −80 °C for further analyses.
Following treatment, rats were anesthetized with 3% isoflurane inhalation (Abbott Laboratories, Chicago, IL, USA) with cardiac blood collected to confirm their death; then, all other major organs including fat pads were removed post-mortem, weighed, snap frozen in liquid nitrogen, and stored at −80 °C for further analyses.
6
High-Fat Diet Induced Metabolic Changes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Trail−/−10 (link) and wildtype mice at 6 w of age, weighing 18–20 g were euthanized for baseline studies, and or randomly grouped and placed on a ‘Western’ high fat diet (HFD; SF00–219; 22% Fat, 0.15% Cholesterol Semi-Pure Rodent Diet) or the Lard diet (SF04–001; 23.5% Fat Semi-Pure Rodent Diet) for 12 w; both from Specialty Feeds, Glen Forest, Western Australia. After an overnight fast, mice were anaesthetized by i.p. injection of ketamine (100 mg/kg) and xylazine (10 mg/kg), or isoflurane (2%) via nose cone prior to euthanasia by cardiac exsanguination. Blood was collected and plasma stored at −80 °C. Gastrocnemius muscle, epididymal WAT, liver, and aorta were collected, fixed in formaldehyde for immunohistochemistry (IHC) or snap-frozen for gene and protein expression. All methods involving animals were carried out in accordance with guidelines and regulations from the National Health and Medical Research Council of Australia; experimental protocols were approved under the Animal Care and Ethics Committees at the University of New South Wales (11/71B) or the Sydney Local Health District (2013/049), Sydney Australia.
7
Obesity and Metabolic Phenotypes in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J WT mice and β2GPI-/- mice on a C57BL/6J background were used in this study [41 (link)]. All animal experimental protocols were approved by the UNSW Animal Care and Ethics Committee. All mice were group housed in the animal house, St George Hospital, University of New South Wales, at 20 +/− 2°C on a 12/12-hour dark-light cycle and had ad libitum access to water and food. Eight-week-old animals (n = 5) for each genotype, diet and sex were randomly allocated into 8 groups (female WT, NC and HF; female β2GPI-/- NC and HF; male WT, NC and HF; male β2GPI-/- NC and HF fed animals) in order to test the influence of genotype, diet and sex on weight gain. NC, was from Gordon's Specialty Stockfeeds, Yanderra, NSW, Australia while the HF, SF00-219 was from Specialty Feeds, Glen Forest, Western Australia, Australia and contained 19.4 kJ/g; energy 40% fat, 17% protein, 43% carbohydrate. The mice were weighed weekly and food intake was measured initially then weekly by monitoring the weight of the remaining food and the mice were sacrificed after 16 weeks of dietary treatment. At the time of sacrifice mice were fasted overnight for 10 - 14 h and anesthetized by isoflurane inhalation.
8
Dietary Impact on Rat Cardiometabolic Health
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Wistar Hooded rats (University of Adelaide, Australia) weighing 180–200 g at the start of the feeding period were housed in groups of four under a light/dark cycle (12/12 h), in a temperature controlled room (22°C) at the RMIT Animal Facility with water ad libitum. Animals were randomly assigned to either a standard (control) diet (SD, Standard AIN93G rodent diet, 7% total fat including 1.05% total saturated fatty acids; Specialty Feeds, Perth, Australia) or WD (SF00-219, 21% total fat including 1.80% total saturated fats and 0.15% cholesterol; Specialty Feeds, Perth, Australia). Animals were allowed ad libitum access to their designated diets for 12 weeks. Food intake and bodyweight were measured weekly. At the end of this period, rats were killed using asphyxiation by CO2 inhalation, followed by decapitation, and their chests were opened to isolate the thoracic aortae. Non-fasted blood samples were obtained from the carotid arteries following decapitation. Blood glucose levels were measured using a one-touch glucometer (Roche, Sydney, NSW, Australia). HbA1c was also measured using the In2it™ A1c system (II) analyzer (Bio-Rad, Hercules, CA, USA).
9
Atherosclerosis Progression in ApoE-/- Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal procedures were approved by the Animal Ethics Committee of the Alfred Medical Research and Education Precinct (AMREP), Melbourne, Australia (Ethic number E/1658/2016/B) and was performed in accordance with the Australian code for care. To investigate the effect of GA on the progression of atherosclerosis, eight weeks old male apolipoprotein E knockout (ApoE−/−) mice (C57Bl/6 background, 25–28 g), ad libitum fed a HFD (22% fat and 0.15% cholesterol, SF00-219, Specialty Feeds, Western Australia, Suppl. Table S1 ) for further eight weeks, have been used. Mice were randomly assigned to receive 1 mg/kg body weight of GA (n = 9) or vehicle (PBS + 0.8% DMSO, n = 9) via intraperitoneal (IP) injection weekly. At the age of 16 weeks, mice have been anesthetized using ketamine (50 mg/kg, Parnell Laboratories, NSW, Australia) and xylazine (10 mg/kg, Troy Laboratories, NSW, Australia). Blood and tissue samples were collected and processed as described below. Grouping of animals and quantifications were blinded from the responsible researchers throughout the study.
10
Hypertensive Rat Diet Intervention
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eight-week-old male SHR and WKY rats (Animal Resource Centre) weighing between 180 and 190 g were euthanized for baseline studies or randomly placed on either normal chow (4.8% fat, 20% protein; Specialty Feeds) or a "Western"-style high-fat diet (22% fat and 0.15% cholesterol; Specialty Feeds SF00-219) for 8 weeks. All rats were housed in groups of 3 in standard-sized housing in an Optimice Hepa Filter System (Animal care Systems) with ad libitum access to food, water and environmental enrichment. Lighting was set to a 12-hour light/dark cycle, and temperature was maintained at 23°C. Rats were monitored daily and weighed weekly. Blood pressure was measured fortnightly using the non-invasive CODA™ mouse/rat tail-cuff system as previously described. 7 Prior to euthanasia, rats were fasted overnight. They were anaesthetised by isoflurane (3% induction and 2% for maintenance) and euthanized via cardiac exsanguination; the collected blood was immediately transferred to a BD Vacutainer blood collection tube containing ethylenediaminetetraacetic acid (EDTA) and placed on ice. Organs including the epididymal white adipose tissue (WAT), retroperitoneal WAT, kidney, spleen and liver were quickly weighed and immediately snap-frozen in liquid nitrogen for gene expression studies or fixed in 10% neutral-buffered formalin (Sigma-Aldrich) overnight for histological assessment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!