The
oligonucleotides used in this study were synthesized and purified
by Sangon Biotech. Co., Ltd.(Shanghai, China) (HPLC). Native PAGE
gel is purchased from Sangon Biotech. Co., Ltd. (Shanghai, China).
The prepared probe is dissolved in TNaK buffer (20 × 10–3 mol/L Tris, pH 7.5; 40 × 10–3 mol/L NaCl;
5 × 10–3 mol/L KCl), annealed, is placed in
a water bath at 95 °C, and naturally cooled to room temperature,
so that the probe maintains the hairpin structure and then, the probe
is placed at -20 °C for standby. All clinical samples were taken
from Zhongda Hospital affiliated to Southeast University. A fluorescence
detection device for CHA-lateral flow immunoassay strip (LFIA) is
GETEIN 1100, purchased from Getein Biotech Inc. (Nanjing, China).
oligonucleotides used in this study were synthesized and purified
by Sangon Biotech. Co., Ltd.(Shanghai, China) (HPLC). Native PAGE
gel is purchased from Sangon Biotech. Co., Ltd. (Shanghai, China).
The prepared probe is dissolved in TNaK buffer (20 × 10–3 mol/L Tris, pH 7.5; 40 × 10–3 mol/L NaCl;
5 × 10–3 mol/L KCl), annealed, is placed in
a water bath at 95 °C, and naturally cooled to room temperature,
so that the probe maintains the hairpin structure and then, the probe
is placed at -20 °C for standby. All clinical samples were taken
from Zhongda Hospital affiliated to Southeast University. A fluorescence
detection device for CHA-lateral flow immunoassay strip (LFIA) is
GETEIN 1100, purchased from Getein Biotech Inc. (Nanjing, China).