Openlab cds chemstation software

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OpenLAB CDS ChemStation software is a data analysis and reporting solution designed for analytical laboratories. It provides tools for data acquisition, processing, and reporting.

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26 protocols using openlab cds chemstation software

Quantitative Analysis of 2,4-D in Urban Soils

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2,4-D concentrations in all liquid samples were determined using
a LC–MS system (Agilent 1260/6150B, Agilent Technologies, USA)
equipped with a Zorbax Eclipse plus C18 column (4.6 × 150 mm
and 3.5 μm dia, Agilent Technologies, USA). The oven temperature
was maintained at 35 °C throughout the analysis. The mobile phases
were 10 mM aqueous ammonium acetate solution (A) and methanol (B).
The mobile phase gradient started at 70% B at 0.0 → 3.0 min,
and went to 95% B at 3.0 → 6.0 min and then 70% B at 6.0 min
following post-run time of 5.0 min with the flow rate set at 0.5 mL
min^–1. The analysis was done in the negative mode
with the single quadrupole mass spectrometer and other set parameters
were: drying gas flow 12.0 mL min^–1 at 300 °C,
nebulizer pressure 35 psi, capillary voltage 4000 V, SIM ion 219 →
221 amu, fragmentor voltage 100 V, and sheath gas flow 3.0 mL min^–1 at 150 °C. The data obtained were processed
using Agilent OpenLAB CDS ChemStation software. The standard curve
was linear over the tested concentration range (R², 0.998). The LOD and LOQ values were 0.007 and 0.03
mg L^–1, respectively. The recovery (mean, n = 3) of spiked 2,4-D for 0.031–0.5 mg L^–1 ranged between 87.98 and 117.94%. Therefore, the method appears
to be reliable and accurate for the determination of the 2,4-D in
different urban soils.

Meftaul I.M., Venkateswarlu K., Dharmarajan R., Annamalai P, & Megharaj M. (2020). Movement and Fate of 2,4-D in Urban Soils: A Potential Environmental Health Concern. ACS Omega, 5(22), 13287-13295.

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Quantitative HPLC-DAD Analysis of Phenolic Compounds

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Aliquots of hydroalcoholic extracts, filtered on 0.45 μm with CA filters, were analyzed by HPLC-DAD using the Agilent 1260 Infinity Series Chromatograph system, supplied with Agilent Open Lab CDS Chem Station Software (Palo Alto, CA, USA). The instrument was equipped with 1260 HIP Degasser, G1312B binary Pump, G1316A Thermostat and G4212B DAD Detector. For separation, analytical 5 μm Phenomenex Luna C18 (4.6 × 250 mm) column (Phenomenex Torrance, CA, USA) was used. The mobile phase was methanol (solvent A) and acetic acid/water (5:95 v/v) (solvent B) and the gradient profile was: 0–25 min, 15–40% A, 25–30 min, 40% A (isocratic), 30–45 min, 40–63% A, 45–47 min, 63% A (isocratic), 47–52 min, 63–100% A, 52–56 min, 100% A (isocratic), with a constant flow of 1 mL/min. Furthermore, the identification of the the main phenolic compounds was performed comparing the spectra and retention time of the pure available standards, as reported by D’Antuono et al. (2018 (link)).

Ramires F.A., Bavaro A.R., D’Antuono I., Linsalata V., D’Amico L., Baruzzi F., Pinto L., Tarantini A., Garbetta A., Cardinali A, & Bleve G. (2023). Liquid submerged fermentation by selected microbial strains for onion skins valorization and its effects on polyphenols. World Journal of Microbiology & Biotechnology, 39(10), 258.

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Vegetable Nitrate and Nitrite Quantification

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The quantitative and qualitative determination of the nitrates and nitrites in the vegetables was performed on an Agilent 1260 HPLC system (Agilent Technologies Singapore (International) Pte.Ltd., Singapore) equipped with a diode array detector (190–900 nm). The instrument was controlled via OpenLab CDS Chemstation Software (Agilent, rev.C.01.05(35)). For the separation, an Agilent Zorbax reversed-phase HPLC column (250 × 4.6 mm; 5 µm) was used with a flow of 1.1 mL/min and an injection volume of 10 µL. For both analytes, the maximum wavelength was 210 nm [31 ]. The limit of quantitation (LOQ) for both the nitrate and nitrate was 1.0 mg kg⁻¹. The mean recovery was 96.0% for nitrate and 95.0% for nitrite, with a linearity (R²) of 0.999 for both analytes. All samples were analyzed in duplicate, with an RSD < 5%.

Luetic S., Knezovic Z., Jurcic K., Majic Z., Tripkovic K, & Sutlovic D. (2023). Leafy Vegetable Nitrite and Nitrate Content: Potential Health Effects. Foods, 12(8), 1655.

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Isoprenoid Quinone Extraction and Quantification

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About 30–50 mg cells were extracted with methanol-chloroform (9:5, v/v) as described before by Hu et al. (1999 (link)) and Seel et al. (2018 (link)) in biological triplicates from independent cultures. Extracts were analysed using a 1260 Infinity Quaternary LC system (Agilent Technologies, USA) equipped with a quaternary pump, an autosampler, a thermo-controlled column compartment, and a diode array detector. Compounds were separated isocratically at 30 °C on a Hypersil™ ODS C18 column (Thermo Fisher, United States) using methanol/diisopropyl ether (9:2, vol/vol) as eluent (flow rate of 1 ml min⁻¹). Isoprenoid quinones were detected at 270 and 275 nm and were identified by their absorption spectrum and retention time. The quinones were quantified as vitamin K₁ equivalents using an external calibration curve and an internal vitamin K₁ standard. Data acquisition was performed with OpenLAB CDS ChemStation software (version C.01.07, Agilent Technologies, USA).

Flegler A., Kombeitz V, & Lipski A. (2021). Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes. Archives of Microbiology, 203(6), 3353-3360.

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Analysis of Plasma Sterols by GC-FID

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As internal standards, 5α-cholestane and epicoprostanol were added to plasma (or LE) samples, and these samples plus standards were saponified with 90% ethanolic sodium hydroxide for 1 hr at 60 °C. After 2 rounds of cyclohexane extraction, samples were derivatized with TMS reagent (pyridine, hexamethyldisilazane, and trimethylchlorosilane (9:3:1, vol/vol/vol)). Derivatized sterols were separated on a DB-XLB capillary column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies, Amstelveen, Netherlands) in an HP6890 plus gas chromatograph fitted with a flame ionization detector. Gas chromatography run conditions were as described elsewhere [21 (link)]. Peaks were identified and integrated using Open Lab CDS Chem Station software (Agilent) and sterol concentrations were calculated relative to the internal standard 5α-cholestane concentration.

Osowska S., Kunecki M., Sobocki J., Tokarczyk J., Majewska K., Burkacka M., Radkowski M., Makarewicz-Wujec M., Fisk H.L., Mashnafi S., Baumgartner S., Plat J, & Calder P.C. (2022). Potential for Omega-3 Fatty Acids to Protect against the Adverse Effect of Phytosterols: Comparing Laboratory Outcomes in Adult Patients on Home Parenteral Nutrition Including Different Lipid Emulsions. Biology, 11(12), 1699.

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Quantifying Murine Hemoglobin Profiles

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Secondary mice were bled 11 weeks post-transplant. One microliter of peripheral blood per was lysed as follows. RBC were pelleted and lysed in Hemolysate reagent (Helena Laboratories, Beaumont, TX) for 5–10 minutes at room temperature. After centrifugation at 20,800g for 10 minutes at 4°C to remove cellular debris, RBC lysates were stored frozen at −80°C. Upon thawing, cell lysates were diluted 1:40 in mobile phase A and characterized by HPLC (Infinity 1260, Agilent) using a weak cation-exchange column (PolyCAT A, PolyLC, Coulmbia, MD). FASC Reference Material (Trinity Biotech, Wicklow, Ireland) was used to define the elution time of common human hemoglobin forms (HbF, HbA, HbS, and HbC). Analysis and peak integration were performed using OpenLAB CDS Chemstation software (Agilent, Santa Clara, CA). The relative percentage of HbAS3 produced for each sample was calculated based on the sum total of areas under the curve for each of the primary hemoglobin peaks, which included βmajor and βminor.

Romero Z., Campo-Fernandez B., Wherley J., Kaufman M.L., Urbinati F., Cooper A.R., Hoban M.D., Baldwin K.M., Lumaquin D., Wang X., Senadheera S., Hollis R.P, & Kohn D.B. (2015). The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. Molecular Therapy. Methods & Clinical Development, 2, 15012-.

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HPLC-MS Protein Analysis Protocol

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HPLC–MS analyses were carried out on an Agilent 1260 Infinity HPLC system, coupled with an Agilent 6120 mass spectrometer [electrospray ionization (ESI) + mode]. The multiply charged envelope was deconvoluted using the charge deconvolution tool in Agilent OpenLab CDS ChemStation software to obtain the average [M] value.

Zhu K., Suskiewicz M.J., Chatrin C., Strømland Ø., Dorsey B.W., Aucagne V., Ahel D, & Ahel I. (2023). DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on nucleic acids. Nucleic Acids Research, 52(2), 801-815.

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HPLC Quantification of Trehalose

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HPLC separation was achieved using
a Waters high-performance carbohydrate column (250 × 4.6 mm², 4 μm) on an Agilent 1100 series HPLC system, including
a degasser, quaternary pump, autosampler, and column compartment (maintained
at 35 °C). The mobile phase was composed of water and acetonitrile.
Isocratic elution conditions were varied to optimize the separation
of trehalose, with an optimal flow rate of 1 mL/min and a percentage
of acetonitrile of 77% over 30 min. The injection volume was 50 μL,
and analyte detection was achieved using an Agilent 1260 Infinity
series refractive index detector. RI signals for trehalose were analyzed
using Agilent OpenLAB CDS ChemStation software; peaks were integrated
manually for consistency. Calibration curves were generated in Excel
by plotting the area of the RI signals against trehalose concentration.
Averages of the areas for each standard were calculated and plotted
against the known concentrations. These plots and the resulting best-fit
equations were used for all further calculations (e.g., determining
the LOD and LOQ, calculating the trehalose concentration of samples,
etc.).

Hayner G.A., Khetan S, & Paulick M.G. (2017). Quantification of the Disaccharide Trehalose from Biological Samples: A Comparison of Analytical Methods. ACS Omega, 2(9), 5813-5823.

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Quantification of Phenolic Compounds in Kale

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Phenolic compounds in kale extract were quantified using an HPLC system (Agilent Technologies 1260 series, Santa Clara, CA, USA) coupled with a diode array detector (DAD). The stationary phase was a C18 reverse phase column, 4.6 mm × 250 mm, 5 μm (Luna, Phenomenex, Torrance, CA, USA). Two mobile phases were used for chromatographic separation: (A) water adjusted to pH 2.4 with phosphoric acid and (B) methanol-water (60:40, v/v). Gradient elution consisted of 0/0, 3/30, 8/50, 35/70, 40/80, 45/100, 50/100 and 60/0 (min/% phase B) at a flow rate of 0.8 mL/min^. The injection volume was 10 μL. The analysis was performed at 280, 320, and 360 nm and integrated by the OpenLAB CDS ChemStation software (Agilent Technologies, Santa Clara, CA, USA).
Peak identification was based on retention time, UV-visible spectra and wavelengths of maximum absorption, as compared with the reported literature [32 (link),81 (link)] and commercial standards. For the quantification of phenolic compounds, standard curves of 4-O-caffeoylquinic acid (0.1–50), ferulic acid (0.4–40 ppm), sinapic acid (0.1–50 ppm), quercetin (0.1–12 ppm), and kaempferol (0.1–12 ppm) were prepared. Results were expressed as mg of each individual phenolic compound per Kg of kale sprouts (mg/Kg) in dry weight (DW) basis.

Ortega-Hernández E., Antunes-Ricardo M., Cisneros-Zevallos L, & Jacobo-Velázquez D.A. (2022). Selenium, Sulfur, and Methyl Jasmonate Treatments Improve the Accumulation of Lutein and Glucosinolates in Kale Sprouts. Plants, 11(9), 1271.

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Solubility Enhancement of SPL Using HP-β-CD

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Phase solubility analysis was performed in purified water containing 0–20% HP-β-CD with an excess of SPL. Each sample was equilibrated in an orbital shaking incubator at 25 °C at 50 rpm for 24 h. Then, they were centrifuged and filtered through a 0.22 µm pore size nylon membrane filter, and the supernatants were diluted before HPLC analysis.
An Agilent (Milan, Italy) 1260 Infinity Quaternary LC System equipped with an Agilent variable wavelength UV detector, a Rheodyne injector (Rheodyne, Model 7725i, Agilent) equipped with a 20 µL loop and OpenLAB CDS ChemStation software (Agilent) were used for HPLC analysis. A Zorbax Eclipse Plus C18 column (particle size 3.5 mm, 4.6 × 100 mm) thermostated at 20 °C was used. An isocratic separation was performed using purified water and ACN, 45/55 v/v, as the mobile phase at a flow rate of 1 mL/min. An injection volume of 20 µL was used, and the SPL was detected at 238 nm. The total acquisition time was 10 min.
Data fitting to determine K_1:1 value was performed using GraphPad/Prism version 5.0 (GraphPad Software, La Jolla, CA, USA) using linear least-squares regression analysis. The stability constant of the complex (K_1:1) and the complex efficiency (CE) were calculated using Equations (1) and (2).

Lopalco A., Manni A., Keeley A., Haider S., Li W., Lopedota A., Altomare C.D., Denora N, & Tuleu C. (2022). In Vivo Investigation of (2-Hydroxypropyl)-β-cyclodextrin-Based Formulation of Spironolactone in Aqueous Solution for Paediatric Use. Pharmaceutics, 14(4), 780.

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