Avance 3 hd 800 mhz nmr spectrometer

Manufactured by Bruker

The Avance III HD 800 MHz NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed and manufactured by Bruker. It is capable of operating at a magnetic field strength of 18.8 Tesla, providing a 1H frequency of 800 MHz. The spectrometer is designed to enable advanced NMR experiments and analysis of complex chemical and biological samples.

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Lab products found in correlation

Topspin 3, by Bruker (1 mentions) Fourier transform infrared spectrometer ft ir 4200, by Jasco (1 mentions) 1200 series high performance liquid chromatography, by Agilent Technologies (1 mentions) Chirascan plus circular dichroism spectrometer, by Applied Photophysics (1 mentions) P 2000 polarimeter, by Jasco (1 mentions)

3 protocols using avance 3 hd 800 mhz nmr spectrometer

RDC NMR Spectroscopy of PopZ Variant

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For RDC NMR experiments, the purified
PopZ^Δ134−177 was buffer exchanged into Buffer F.
The protein was concentrated and D₂O, NaN₃, and DSS were
added to a 5% v/v, 4 mM, and 0.2 mM concentration, respectively, at a final
PopZ^Δ134−177 concentration of 0.20 mM. The protein
sample was used to rehydrate a 5.4 mm or 6.0 mm (outer diameter) gel previously
cast and dehydrated (see RDC gel
preparation below). The gel was stretched into a New Era Enterprises
4.2 mm (inner diameter) NMR tube for RDC NMR experiments. The
¹H-¹⁵N HSQC NMR spectrum was collected at 25 °C
on a Bruker AVANCE III HD 800 MHz NMR spectrometer equipped with a 5-mm triple
resonance inverse TCI CryoProbe.

Nordyke C.T., Ahmed Y.M., Puterbaugh R.Z., Bowman G.R, & Varga K. (2020). Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature. Journal of molecular biology, 432(23), 6092-6107.

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NMR Spectroscopy Analysis of ARPP-PP2A Interaction

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The ¹⁵N-labeled ARPP proteins were titrated with the unlabeled A-subunit of PP2A. The proportion of ARPP-16 and the A-subunit of PP2A (ARPP-16: PP2A A-subunit) used in the titration were 1:0.5 (200:100 μM), 1:1 (162:170 μM), 1:2 (200:400 μM) and 1:3 (160:480 μM). The proportion of ARPP-19 and the A-subunit of PP2A (ARPP-19: PP2A A-subunit) used in the titration tests were 1:0.5 (200:100 μM), 1:1 (180:180 μM), 1:2 (160:320 μM) and 1:3 (140:450 μM). All proteins were purified in 50 mM NaH₂PO₄, 100 mM NaCl, 1 mM DTT, pH 6.8. ¹⁵N-HSQC titration experiments were performed on a Bruker Avance III HD 800 MHz NMR spectrometer at 25°C. All spectra were processed with TopSpin 3.5 software package (Bruker Corporation, Billerica, MA, United States) and analyzed using NMRFAM-Sparky 3.13 (Lee et al., 2014 (link)).

Thapa C., Roivas P., Haataja T., Permi P, & Pentikäinen U. (2021). The Interaction Mechanism of Intrinsically Disordered PP2A Inhibitor Proteins ARPP-16 and ARPP-19 With PP2A. Frontiers in Molecular Biosciences, 8, 650881.

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Comprehensive Characterization of Molecular Structure

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Specific rotation was measured by a JASCO P-2000 polarimeter with a 10 mm cell at 20 °C. Ultraviolet (UV) data were acquired by an Applied Photophysics Chirascan-Plus circular dichroism spectrometer using a 1 mm UV cell. Infrared (IR) spectra were recorded by a JASCO Fourier transform/infrared spectrometer (FT/IR-4200). One-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectra were obtained by a Bruker Avance III HD 800 MHz NMR spectrometer located at the College of Pharmacy, Seoul National University, Republic of Korea. Chemical shifts of all NMR spectra were referenced to the residual protonated solvent peaks of dimethyl sulfoxide (DMSO)-d₆ (δ_H 2.50/δ_C 39.5). Liquid chromatography/mass spectrometry (LS/MS) data and low-resolution electrospray ionization mass spectrometry (LRESIMS) data were acquired using an Agilent Technologies 1200 series high-performance liquid chromatography (HPLC) coupled with an Agilent Technologies 6130 series single quadrupole ESIMS instrument. High-resolution ESIMS (HRESIMS) experiments were carried out on an AB Sciex 5600 quadrupole time-of-flight (QTOF) HRMS instrument at the National Instrumentation Center for Environmental Management (NICEM), Seoul National University, Republic of Korea.

Kang S., Han J., Jang S.C., An J.S., Kang I., Kwon Y., Nam S.J., Shim S.H., Cho J.C., Lee S.K, & Oh D.C. (2022). Epoxinnamide: An Epoxy Cinnamoyl-Containing Nonribosomal Peptide from an Intertidal Mudflat-Derived Streptomyces sp.. Marine Drugs, 20(7), 455.

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