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L cysteine standard

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The L-cysteine standard is a high-purity reference material used for the quantitative analysis and identification of L-cysteine in various applications. It provides a known concentration of L-cysteine to be used as a calibration standard or quality control sample.

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Lab products found in correlation

Ultrapure water, by Thermo Fisher Scientific (2 mentions) Ellman s reagent, by Thermo Fisher Scientific (2 mentions) 8 arm 20 kda peg vinyl sulfone peg vs macromers, by JenKem Technology (2 mentions)

Spelling variants of this product

L-cysteine (59 citations) Cysteine (21 citations)

2 protocols using l cysteine standard

1

Peptide-Functionalized PEG Hydrogels for Tissue Engineering

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
8-arm 20 kDa PEG vinyl sulfone (PEG-VS) macromers were purchased from JenKEM Technology (Beijing). All peptides were custom synthesized and purified (>95%) by Aapptec or Genscript. Following peptide were used in this study 16 ,19 (link): “MMP-CL”, a dithiol crosslinking peptide containing a matrix metalloproteinase (MMP)- and Sortase-sensitive recognition site, (Ac)GCRD-LPRTG-GPQGIWGQ-DRCG(Am); “PHSRN-K-RGD”, a fibronectin (FN) derived adhesion peptide containing the PHSRN synergy site and the RGD motif from the 9th and 10th FN type III repeat respectively in a branched assembly, (Ac)PHSRNGGGK-[GGGERCG(Ac)]-GGRGDSPY(Am)40 (link), labelled with the single letter “F” in the gels; “GFOGER”, a triple-helical collagen-I derived adhesion peptide, (Ac)GGYGGGPG(GPP)5GFOGER(GPP)5GPC(Am)17 (link), labelled with the letter “C” in the gels; “BM-binder”, a peptide with high affinity for BM-derived proteins specifically collagen type IV and laminin, (Ac)GCRE-ISAFLGIPFAEPPMGPRRFLPPEPKKP(Am)40 (link), labelled with the letter “B” in the gels. All peptides were reconstituted in ultrapure water (Thermo) at a concentration of 20 mM for all adhesion mimetic and BM-binding peptides and 50 mM for the MMP-CL crosslinker. The concentration of free thiols was determined using the Ellman’s Reagent (Thermo) according to the manufacturer’s instructions using an L-cysteine standard (Thermo).
Below C.R., Kelly J., Brown A., Humphries J.D., Hutton C., Xu J., Lee B.Y., Cintas C., Zhang X., Hernandez-Gordillo V., Stockdale L., Goldsworthy M.A., Geraghty J., Foster L., O’Reilly D.A., Schedding B., Askari J., Burns J., Hodson N., Smith D.L., Lally C., Ashton G., Knight D., Mironov A., Banyard A., Eble J.A., Morton J.P., Humphries M.J., Griffith L.G, & Jørgensen C. (2021). A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids. Nature materials, 21(1), 110-119.
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2

Peptide-Functionalized PEG Hydrogels for Tissue Engineering

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
8-arm 20 kDa PEG vinyl sulfone (PEG-VS) macromers were purchased from JenKEM Technology (Beijing). All peptides were custom synthesized and purified (>95%) by Aapptec or Genscript. Following peptide were used in this study 16 ,19 (link): “MMP-CL”, a dithiol crosslinking peptide containing a matrix metalloproteinase (MMP)- and Sortase-sensitive recognition site, (Ac)GCRD-LPRTG-GPQGIWGQ-DRCG(Am); “PHSRN-K-RGD”, a fibronectin (FN) derived adhesion peptide containing the PHSRN synergy site and the RGD motif from the 9th and 10th FN type III repeat respectively in a branched assembly, (Ac)PHSRNGGGK-[GGGERCG(Ac)]-GGRGDSPY(Am)40 (link), labelled with the single letter “F” in the gels; “GFOGER”, a triple-helical collagen-I derived adhesion peptide, (Ac)GGYGGGPG(GPP)5GFOGER(GPP)5GPC(Am)17 (link), labelled with the letter “C” in the gels; “BM-binder”, a peptide with high affinity for BM-derived proteins specifically collagen type IV and laminin, (Ac)GCRE-ISAFLGIPFAEPPMGPRRFLPPEPKKP(Am)40 (link), labelled with the letter “B” in the gels. All peptides were reconstituted in ultrapure water (Thermo) at a concentration of 20 mM for all adhesion mimetic and BM-binding peptides and 50 mM for the MMP-CL crosslinker. The concentration of free thiols was determined using the Ellman’s Reagent (Thermo) according to the manufacturer’s instructions using an L-cysteine standard (Thermo).
Below C.R., Kelly J., Brown A., Humphries J.D., Hutton C., Xu J., Lee B.Y., Cintas C., Zhang X., Hernandez-Gordillo V., Stockdale L., Goldsworthy M.A., Geraghty J., Foster L., O’Reilly D.A., Schedding B., Askari J., Burns J., Hodson N., Smith D.L., Lally C., Ashton G., Knight D., Mironov A., Banyard A., Eble J.A., Morton J.P., Humphries M.J., Griffith L.G, & Jørgensen C. (2021). A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids. Nature materials, 21(1), 110-119.
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