Ms grade porcine pancreatic trypsin

GSH, GSSG, Sepasol-RNA I Super G, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Nacalai Tesque Inc. (Kyoto, Japan). ReverTra Ace was procured from Toyobo Co., Ltd. (Osaka, Japan). Fast SYBR Green Master Mix and the BCA protein assay kit were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Transaminase CII-test Wako and MS-grade porcine pancreatic trypsin were obtained from Fujifilm Wako Pure Chemical Co., Ltd. (Osaka, Japan). The QuantiChrom bilirubin assay kit was obtained from BioAssay Systems, LLC. (Hayward, CA, USA). Percoll was obtained from Cytiva (Tokyo, Japan). LPS from Escherichia coli was procured from Sigma-Aldrich Co., LLC. (St. Louis, MO, USA). LPS-RS was obtained from InvivoGen Inc. (San Diego, CA, USA). FPS-ZM1 was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Oligonucleotide primers were obtained from Eurofins Genomics Inc. (Luxembourg, Luxembourg). All other chemicals and solvents were of MS grade or higher and of commercially available purity.

Dulbecco’s modified Eagle’s medium (DMEM), Sepasol-RNA I Super G, and PMA were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). IFN-γ, IL-4, and IL-13 were obtained from R&D systems, Inc. (Minneapolis, MN, USA). DIC, IBP, NAP, ZOM, and LPS from Escherichia coli were purchased from Sigma-Aldrich (St. Louis, MO, USA). ReverTra Ace was purchased from Toyobo Co., Ltd. (Osaka, Japan). The fast SYBR Green Master Mix and BCA protein assay kit were from Thermo Fisher Scientific (Waltham, MA, USA). Random primers, dNTPs, and LDH Cytotoxicity Detection Kit were purchased from Takara Holdings Inc. (Shiga, Japan). FLU, KET, RPMI-1640, and MS-grade porcine pancreatic trypsin were obtained from Fujifilm Wako Pure Chemical Co., Ltd. (Osaka, Japan). IBF was from Cayman Chemical Co. (Ann Arbor, MI, USA). The (glutathione disulfide) GSSG/GSH Quantification Kit was obtained from Dojindo Laboratories Co., Ltd. (Kumamoto, Japan). The Human HMGB1 ELISA kit was obtained from Arigo Biolaboratories Co. (Hsinchu City, Taiwan). Oligonucleotide primers were from Eurofins genomics, Inc. (Luxembourg, Luxembourg). All other chemicals and solvents were of MS grade or the highest commercially available purity.