Anti lamin a c n 18

Manufactured by Santa Cruz Biotechnology

Anti-LAMIN A/C (N-18) is a primary antibody specific for the N-terminal region of the Lamin A/C protein. Lamin A/C is a structural protein that is a component of the nuclear lamina, which provides mechanical support and organization to the cell nucleus.

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Lab products found in correlation

Anti β actin, by Santa Cruz Biotechnology (2 mentions) Anti lamin a c e1, by Santa Cruz Biotechnology (2 mentions) Ab11419, by Abcam (1 mentions) Ab53068, by Abcam (1 mentions) Ab37168, by Abcam (1 mentions) Adi spa 827, by Enzo Life Sciences (1 mentions) Spa 860, by Enzo Life Sciences (1 mentions) F7425, by Merck Group (1 mentions) Anti p eif2α, by Cell Signaling Technology (1 mentions) Mms 435p, by Fortrea (1 mentions) Ab92446, by Abcam (1 mentions) Ab92307, by Abcam (1 mentions) Pa5 19542, by Thermo Fisher Scientific (1 mentions) P histone h2a x s139, by Cell Signaling Technology (1 mentions) P histone h3, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Lamin A/C (177 citations) Anti-Lamin A/C (73 citations) Lamin A (27 citations) Anti-Lamin A (18 citations)

5 protocols using anti lamin a c n 18

Immunoblotting Antibody Dilutions

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The following antibodies were used for immunoblotting at the indicated dilution: Anti-LAMIN A/C (N-18) 1:100 (SantaCruz, Catalogue No: sc6215), Anti-LAMIN A/C (E-1) 1:100 (SantaCruz, Catalogue No: sc376248), anti-β actin 1: 600 (SantaCruz, Catalogue No: sc47778), anti-α tubulin 1: 750 (Calbiochem, Catalogue No: CP06). HRP-conjugated secondary antibodies were anti-goat 1:500 (Novex, Catalogue No: A15909), anti-mouse 1:1000 (Cell Signaling, Catalogue No: 7076)

Beyret E., Liao H.K., Yamamoto M., Hernandez-Benitez R., Fu Y., Erikson G., Reddy P, & Belmonte J.C. (2019). Single-Dose CRISPR/Cas9 Therapy Extends Lifespan of Mice with Hutchinson-Gilford Progeria Syndrome. Nature medicine, 25(3), 419-422.

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Antibody Characterization for Cell Culture

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Reagents, buffers, culture media and serum for cell cultures were from Sigma–Aldrich unless stated otherwise.
The following commercial antibodies were used: anti-NLGN pan-mouse monoclonal antibody (clone 4F9, catalogue number 129-011, Synaptic Systems), anti-p-eIF2α (eukaryotic initiation factor 2α) (Ser⁵¹) rabbit antibody (119A11, Cell Signaling Technology), anti-eIF2α total mouse antibody (L57A5, Cell Signaling Technology), anti-BiP rabbit polyclonal antibody (ab53068, Abcam), anti-CHOP mouse monoclonal antibody (ab11419, Abcam), rabbit polyclonal anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (ab37168, Abcam), goat polyclonal anti-lamin A/C (N18, Santa Cruz Biotechnology), mouse monoclonal anti-KDEL (ADI-SPA-827, Enzo Life Sciences), mouse βIII-tubulin (MMS-435P, Covance), rabbit anti-FLAG (F7425, Sigma), mouse anti-FLAG M2 monoclonal antibody (F3165, Sigma) and rabbit anti-calnexin polyclonal antibody (SPA-860, Enzo Life Sciences). Goat secondary anti-rabbit IgG (rhodamine-conjugated) and anti-mouse IgG (FITC-conjugated) were from Abcam.

Ulbrich L., Favaloro F.L., Trobiani L., Marchetti V., Patel V., Pascucci T., Comoletti D., Marciniak S.J, & De Jaco A. (2016). Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochemical Journal, 473(Pt 4), 423-434.

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Comprehensive Nuclear Protein Analysis

Cited 1 time

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The following primary antibodies were used: anti-SATB2 (ab92446, Abcam); anti-SATB1 (ab92307, Abcam); anti-HDAC1 (10E2) (5356P, Cell signaling); anti-HNRNPL (ab156682, Abcam); anti-HNRNPL-like (4783S, Cell signaling); anti-HNRNPC1/C2 (M022726, BOSTER); anti-ZNF638 (orb215138, Biorbyt); anti-DHX9 (PA5-19542, Thermo Scientific); anti-CUX1 (M-222) (sc-13024, Santa Cruz); anti-Lamin A/C (N-18) (sc-6215, Santa Cruz); anti-ERK2 (C-14) (sc-154, Santa Cruz), anti-BANF1 (PU38143, a generous gift from T. Haraguchi [48 (link)]; anti-pan LAP2 (Lap2 2–12), a generous gift from R. Foisner [49 (link)], anti-CHMP3 (HPA015673, Atlas Antibodies).

Cera I., Whitton L., Donohoe G., Morris D.W., Dechant G, & Apostolova G. (2019). Genes encoding SATB2-interacting proteins in adult cerebral cortex contribute to human cognitive ability. PLoS Genetics, 15(2), e1007890.

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Multiparametric Analysis of DNA Damage Signaling

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Chk1 (A300-162A) and p-Chk1 (S296) (2349S), p-Histone H3 (S10) (D2C8)(Alexa®647), p-Histone H2A.X (S139) (20E3, 9718S), ATM (pS1981) (2152-1) (Cell Signaling. Danvers, MA), phospoho KAP-1 (S824) (A300-767A) and KAP1 (Cat # A300-274A), anti-ATM (A300-136A), (Bethyl, Montgomery, TX), Gout Polyclonal anti-WWOX antibody (a gift of Dr. Kay Huebner), anti-GAPDH mouse mAB (CB1001), anti-HSP90 rabbit (Cat # CA1016) (CALBIOCHEM, Billerica, MA), anti-Lamin A/C (N-18, Santa Cruz Biotechnology. Dallas, TX).

Abu-Odeh M., Hereema N.A, & Aqeilan R.I. (2015). WWOX modulates the ATR-mediated DNA damage checkpoint response. Oncotarget, 7(4), 4344-4355.

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Immunoblotting Antibody Dilutions

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