Sodium dextran sulfate

Manufactured by Merck Group

Sourced in Germany, United States

Sodium dextran sulfate is a laboratory reagent used as a precipitating agent for the isolation and purification of nucleic acids, particularly DNA and RNA. It is a complex polysaccharide derived from the naturally occurring carbohydrate dextran, which has been chemically modified by the introduction of sulfate groups. This modification enhances the precipitating properties of the compound, allowing it to effectively remove nucleic acids from complex biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Protease from streptomyces griseus type 6 no p 5130, by Merck Group (2 mentions) Poly l arginine hydrochloride parg, by Merck Group (2 mentions) Potassium poly vinyl sulfate, by Merck Group (2 mentions) Sodium polystyrene sulfonate, by Merck Group (2 mentions) Poly l lysine hydrobromide, by Merck Group (1 mentions)

Spelling variants of this product

Sodium sulfate (279 citations) DSS (216 citations) Dextran sulfate (109 citations) Dextrans (38 citations) Dextran sulfate sodium salt (35 citations) Dextran sulfate sodium salt (DSS, (9 citations) Dextran sulfate sodium salt from Leuconostoc spp. (2 citations)

5 protocols using sodium dextran sulfate

Microbial Protease Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Poly-L-lysine hydrobromide (PL, MW 70,000), poly-L-arginine hydrochloride (PArg, MW 15,000–70,000) and sodium dextran sulfate (DS, MW 50,000) were from Sigma (Neustadt, Germany); bacteriophage of Proteus vulgaris and Escherichia coli (Microgen NPO AO, Microgen, Moscow, Russia), ethylenediaminetetraacetic acid (EDTA), calcium chloride (CaCl₂·2H₂O), sodium chloride and sodium carbonate were from Reahim (Ekaterinburg, Russia). Protease from Streptomyces griseus (Type VI No. P-5130) was obtained from Sigma (Neustadt, Germany).

Musin E.V., Kim A.L., Dubrovskii A.V., Ariskina E.V., Kudryashova E.B, & Tikhonenko S.A. (2022). The Pathways to Create Containers for Bacteriophage Delivery. Polymers, 14(3), 613.

+ Open protocol

+ Expand

Viral Capsid Functionalization Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dextran (D, MW 40,000) Sigma (Merck KGaA, Darmstadt, Germany), Poly-L-arginine hydrochloride (PArg, MW 15,000–70,000) Sigma (Merck KGaA, Darmstadt, Germany) and sodium dextran sulfate (DS, MW 15,000) Sigma (Merck KGaA, Darmstadt, Germany), polystyrenesulfonate sodium (PSS) and polyallylamine hydrochloride (PAH) with a molecular mass of 70 kDa, coliprotetic bacteriophage (Microgen NPO AO, Moscow, Russia), ethylenediaminetetraacetic acid (EDTA), calcium chloride (CaCl₂ × 2H₂O), sodium chloride and sodium carbonate from Reahim (Reahim AO, St.Petersburg, Russian Federation). Protease from Streptomyces griseus (Type VI No. P-5130) Sigma (Merck KGaA, Darmstadt, Germany).

Musin E.V., Kim A.L., Dubrovskii A.V., Kudryashova E.B., Ariskina E.V, & Tikhonenko S.A. (2021). The Influence of Polyanions and Polycations on Bacteriophage Activity. Polymers, 13(6), 914.

+ Open protocol

+ Expand

Polyelectrolyte Characterization and Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Structures of the used polymers are shown in Scheme 1. Samples of sodium poly(styrene sulfonate) (PSS) with a polymerization degree of 1700 (350 kDa), sodium poly(anethole sulfate) (PAS) with an approximate polymerization degree of 800 (200 kDa), sodium dextran sulfate (DS) with a molecular mass of 100 kDa (approximately 250 repeated units and 600 charged groups), potassium poly(vinyl sulfate) (PVS) with an approximate polymerization degree of 1100 (180 kDa) and polymethacrylic (PMA) acid with an approximate polymerization degree of 1800 (150 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Poly(N-ethyl-4-vinylpyridinium) bromide (PEVP) with a polymerization degree of 1600 (340 kDa), as well as a statistical copolymer of N-ethyl-4-vinylpyridinium bromide and 4-vinylpyridine (PEVP-50%) were synthesized by alkylation of the poly(4-vinylpyridinium) sample with ethyl bromide as described in [46 (link)] and kindly provided by Prof. Vladimir Izumrudov from Lomonosov Moscow State University (Moscow, Russia). Polyelectrolyte concentrations were expressed in the terms of the molar concentration of charged groups excepting PEVP-50%, in which the molar concentration of chains was the same as PEVP.

Semenyuk P., Kurochkina L., Barinova K, & Muronetz V. (2020). Alpha-Synuclein Amyloid Aggregation Is Inhibited by Sulfated Aromatic Polymers and Pyridinium Polycation. Polymers, 12(3), 517.

+ Open protocol

+ Expand

LDL and VLDL Binding and Uptake in Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated primary hepatocytes of WT and Lrp6^mut/mut mice were serum starved overnight, followed by treatment with ¹²⁵I-LDL or ¹²⁵I-VLDL (Biomedical Technologies, Inc.). For binding assays, cells were pre-chilled at 4 °C followed by adding 10 μg/mL ¹²⁵I-LDL in DMEM supplemented with lipoprotein-deficient serum for 2 h at 4 °C. Cells were incubated with 2 mL of sodium dextran sulfate (4 mg/mL, Sigma). An aliquot was placed in gamma counter to determine the total amount of ¹²⁵I-LDL bound to the cell surface. Cells were harvested and the lysate was used to measure protein concentration. For LDL or VLDL uptake, cells were incubated in lipoprotein-deficient serum with 10 μg/mL ¹²⁵I-LDL or ¹²⁵I-VLDL for 2 h at 37 °C. Harvested cell lysate was applied for radioactivity determination as described previously.

Go G.W., Srivastava R., Hernandez-Ono A., Gang G., Smith S.B., Booth C.J., Ginsberg H.N, & Mani A. (2014). The Combined Hyperlipidemia Caused by Impaired Wnt-LRP6 signaling is reversed by Wnt3a Rescue. Cell metabolism, 19(2), 209-220.

+ Open protocol

+ Expand

Polyelectrolyte Polymers in Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Structures of the used polymers (that all are strong polyelectrolytes and presented as polyanions) are shown in Scheme 1. Samples of sodium poly(styrene sulfonate) (PSS) with polymerization degree of 155 and 1700 (32 and 350 kDa, respectively), sodium poly(anethole sulfonate) (PAS) with approximate polymerization degree of 800 (200 kDa), sodium dextran sulfate (DS) with molecular mass of 15 kDa and 100 kDa (approximately 38 and 250 repeated units or 90 and 600 charged groups, respectively), and potassium poly(vinyl sulfate) (PVS) with approximate polymerization degree of 1100 (180 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Semenyuk P., Evstafyeva D., Izumrudov V, & Muronetz V. (2022). Synthetic Sulfated Polymers Control Amyloid Aggregation of Ovine Prion Protein and Decrease Its Toxicity. Polymers, 14(7), 1478.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!