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Sc-281 is a laboratory instrument designed for the detection and analysis of specific proteins or molecules in biological samples. It utilizes advanced technology to provide accurate and reliable results. The core function of Sc-281 is to facilitate the identification and quantification of target analytes, supporting various research and diagnostic applications.

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Lab products found in correlation

Amersham ecl start western blotting detection reagent, by GE Healthcare (2 mentions) Ab9385, by Abcam (2 mentions) Bca assay, by Thermo Fisher Scientific (2 mentions) Pn66485, by Avantor (2 mentions) Bolt 4 12 bis tris plus gel, by Thermo Fisher Scientific (2 mentions) Fusion solo, by Vilber (2 mentions)

2 protocols using sc 281

1

Western Blot Analysis of SLC9A3R1 and YY

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed twice in ice-cold PBS and lysed in RIPA (Sigma-Aldrich; R02780) buffer supplemented with protease (Roche 11697498001) and phosphastase (Sigma-Aldrich 93482) inhibitors for 30 minutes with intermittent vortexing. Samples were centrifuged at 4°C at maximum speed for 30 minutes after which, the supernatant is transferred to a clean Eppendorf. Protein concentrations for each sample was ascertained using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific; 23227). Equal amounts of lysates were loaded into BOLT 4-12% Bis-Tris Plus Gel (Invitrogen; NW04120BOX). Proteins were transferred to a Biotrace nitrocellulose membrane (VWR; PN66485) and incubated with primary antibodies overnight. Proteins were then visualised using goat anti-mouse (ThermoFisher Scientific; 31446) and anti-rabbit (ThermoFisher Scientific; 31462) HRP conjugated secondary antibodies. Amersham ECL start Western Blotting Detection reagent (GE Healthcare Life Sciences; RPN3243) was used for chemiluminescent imaging using the Fusion solo (Vilber; Germany) imager. For SLC9A3R1 we used HPA027247 (protein atlas) at 1:1000 dilution, for YY we used Santa Cruz; sc-281 at 1:500 dilution. For GAPDH we used Abcam #ab9385 at 1:5000 dilution.
Patten D.K., Corleone G., Győrffy B., Perone Y., Slaven N., Barozzi I., Erdős E., Saiakhova A., Goddard K., Vingiani A., Shousha S., Pongor L.S., Hadjiminas D.J., Schiavon G., Barry P., Palmieri C., Coombes R.C., Scacheri P., Pruneri G, & Magnani L. (2018). Enhancer mapping uncovers phenotypic heterogeneity and evolution in patients with luminal breast cancer. Nature medicine, 24(9), 1469-1480.
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2

Western Blot Analysis of SLC9A3R1 and YY

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed twice in ice-cold PBS and lysed in RIPA (Sigma-Aldrich; R02780) buffer supplemented with protease (Roche 11697498001) and phosphastase (Sigma-Aldrich 93482) inhibitors for 30 minutes with intermittent vortexing. Samples were centrifuged at 4°C at maximum speed for 30 minutes after which, the supernatant is transferred to a clean Eppendorf. Protein concentrations for each sample was ascertained using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific; 23227). Equal amounts of lysates were loaded into BOLT 4-12% Bis-Tris Plus Gel (Invitrogen; NW04120BOX). Proteins were transferred to a Biotrace nitrocellulose membrane (VWR; PN66485) and incubated with primary antibodies overnight. Proteins were then visualised using goat anti-mouse (ThermoFisher Scientific; 31446) and anti-rabbit (ThermoFisher Scientific; 31462) HRP conjugated secondary antibodies. Amersham ECL start Western Blotting Detection reagent (GE Healthcare Life Sciences; RPN3243) was used for chemiluminescent imaging using the Fusion solo (Vilber; Germany) imager. For SLC9A3R1 we used HPA027247 (protein atlas) at 1:1000 dilution, for YY we used Santa Cruz; sc-281 at 1:500 dilution. For GAPDH we used Abcam #ab9385 at 1:5000 dilution.
Patten D.K., Corleone G., Győrffy B., Perone Y., Slaven N., Barozzi I., Erdős E., Saiakhova A., Goddard K., Vingiani A., Shousha S., Pongor L.S., Hadjiminas D.J., Schiavon G., Barry P., Palmieri C., Coombes R.C., Scacheri P., Pruneri G, & Magnani L. (2018). Enhancer mapping uncovers phenotypic heterogeneity and evolution in patients with luminal breast cancer. Nature medicine, 24(9), 1469-1480.
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