Epilactose

Manufactured by Merck Group

Sourced in United States

Epilactose is a laboratory equipment product designed for the analysis and quantification of lactose in various samples. It functions as a tool for researchers and analysts to accurately measure lactose content in their experiments or testing procedures.

Automatically generated - may contain errors

Lab products found in correlation

Lactulose, by Merck Group (4 mentions) Electrophoresis reagents, by Bio-Rad (4 mentions) Chelating sepharose fast flow resin, by GE Healthcare (2 mentions) Escherichia coli bl21 de3, by New England Biolabs (1 mentions) Ex taq polymerase, by Takara Bio (1 mentions) T4 dna ligase, by Takara Bio (1 mentions) Lactose, by Merck Group (1 mentions) Escherichia coli bl21 de3, by Promega (1 mentions) Escherichia coli bl21 de3 plyss, by Promega (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Sangon (1 mentions) Phusion hf dna polymerase, by New England Biolabs (1 mentions)

5 protocols using epilactose

Cloning and Characterizing Carbohydrate-Active Enzymes

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The restriction endonucleases NheI and EcoRI, T4 DNA ligase, and EX taq polymerase were obtained from Takara (Kyoto, Japan). The expression vector pET28a (+) was obtained from Novagen (Darmstadt, Germany). Plasmid DNA was purified by using DNA mini-preparation kits (Qiagen, Hilden, Germany). Escherichia coli BL21 (DE3) (New England Biolabs, Hertfordshire, UK) was used for protein expression. C. saccharolyticus DSM 8903 cell was purchased from DSMZ (Braunschweig, Germany). Standard sugars (lactose, epilactose, and lactulose) for high-performance liquid chromatography (HPLC) were purchased from Sigma-Aldrich (MO, USA).

Park A.R., Kim J.S., Jang S.W., Park Y.G., Koo B.S, & Lee H.C. (2017). Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus. Microbial Cell Factories, 16, 224.

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Enzymatic Synthesis of Galactooligosaccharides

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Unless otherwise stated, all materials and reagents were of analytical grade or higher supplied by Sinopharm Chemical Reagent (Shanghai, China). Isopropyl β-d-1-thiogalactopyranoside (IPTG) and ampicillin sodium were supplied by Sangon Biological Engineering Technology and Services (Shanghai, China). Electrophoresis reagents were obtained from Bio-Rad (Hercules, CA, USA). Escherichia coli BL21 (DE3) and DH5α were obtained from Promega (Madison, WI). The Ni²⁺-chelating Sepharose Fast Flow resin was purchased from GE Healthcare (Uppsala, Sweden). Epilactose and lactulose were of the highest grade and were obtained from Sigma (St Louis, MO, USA). Cheese whey powder was purchased from Apple Foods Tech (Shanghai, China).

Chen Q., Xiao Y., Shakhnovich E.I., Zhang W, & Mu W. (2019). Semi-rational design and molecular dynamics simulations study of the thermostability enhancement of cellobiose 2-epimerases. International journal of biological macromolecules, 154, 1356-1365.

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Recombinant Enzyme Characterization

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Unless otherwise stated, all materials and reagents were of analytical grade or higher purchased from Sinopharm Chemical Reagent (Shanghai, China). Epilactose and lactulose were of the highest grade and acquired from Sigma (St Louis, MO, USA). Isopropyl β-D-l-thiogalactopyranoside (IPTG), ampicillin sodium and other chemicals used for enzyme assays and characterizations were purchased from Sangon Biotech (Shanghai, China). Electrophoresis reagents were supplied by Bio-Rad. The competent cells of bacterial strain Escherichia coli BL21 (DE3) pLysS was purchased from Promega (Madison, WI). Chelating Sepharose Fast Flow resin was from GE Healthcare (Uppsala, Sweden).

Xiao Y., Chen Q., Shakhnovich E.I., Zhang W, & Mu W. (2019). Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase. International journal of biological macromolecules, 139, 1002-1008.

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Recombinant Protein Production in E. coli

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Isopropyl β-d-1-thiogalactopyranoside and ampicillin sodium salt were purchased from Sangon Biotech (Shanghai, China). Electrophoresis reagents were purchased from Bio-Rad (Hercules, CA). The competent cells of E. coli BL21 (DE3) used for the cloning and expression of the proteins were purchased from Promega (Madison, WI). Chelating Sepharose Fast Flow resin was supplied by GE Healthcare (Uppsala, Sweden). Cheese whey powder was obtained from Apple Foods Tech (Shanghai, China). Epilactose and lactulose were of analytical grade and were obtained from Sigma-Aldrich (St. Louis, MO). All other materials and reagents were purchased from Sinopharm Chemical Reagent (Shanghai, China).

Chen Q., Xiao Y., Zhang W., Stressler T., Fischer L., Jiang B, & Mu W. (2020). Computer-aided search for a cold-active cellobiose 2-epimerase. Journal of dairy science, 103(9).

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Enzymatic Cheese Whey Processing

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Epilactose and gel filtration chromatography standards were purchased from Sigma-Aldrich (St Louis, MO). Cheese whey powder was purchased from Apple Foods Tech (Shanghai, China). Ethanol to permeabilize the cells was obtained from Feizhidao Food Ingredients Factory (Henan, China), and β-galactosidase (EC 3.2.1.23) from Bifidobacterium bifidum purchased from Sangon Biotech (Shanghai, China); both were foodgrade. The electrophoresis reagents were from Bio-Rad (Hercules, CA). Phusion HF DNA Polymerase was from New England Biolabs (Beijing, China). The primers for PCR amplification and all other chemicals in this study were obtained from Sangon Biotech (Shanghai, China).

Chen Q., He W., Yan X., Zhang T., Jiang B., Stressler T., Fischer L, & Mu W. (2018). Construction of an enzymatic route using a food-grade recombinant Bacillus subtilis for the production and purification of epilactose from lactose. Journal of dairy science, 101(3).

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