HEK293T cells were obtained from ATCC and then maintained with DMEM (Gibco, Gaithersburg, MD) containing 10% FBS (Sigma). The SCLC cell lines were obtained from ATCC. NCI-H748, NCI-H1963, NCI-H209, NCI-H889, and NCI-H69 cells were maintained with ATCC-formulated RPMI-1640 medium containing 10% FBS (Sigma). NCI-H1882, NCI-H1436, NCI-H1105, and NCI-H2171 cells were maintained with ATCC-formulated DMEM/F12 cell culture media containing 10% FBS (Sigma).
Dmem f12 cell culture media
Manufactured by Merck Group
DMEM/F12 is a cell culture media formulation that provides a balanced set of nutrients essential for the growth and maintenance of a variety of cell types in vitro. It is a widely used media that supports the cultivation of both adherent and suspension cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t cell, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Sclc cell lines, by American Type Culture Collection (1 mentions)
Sk br 3, by Merck Group (1 mentions)
Hek293, by Merck Group (1 mentions)
Mda mb 468, by Merck Group (1 mentions)
Rpmi 1640 cell culture media, by Merck Group (1 mentions)
Bt 20, by Merck Group (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Bt 20, by American Type Culture Collection (1 mentions)
3 protocols using dmem f12 cell culture media
1
Maintenance of HEK293T and SCLC Cell Lines
2
Cell Line Maintenance and Validation
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HEK293T cells were obtained from ATCC, and then maintained with DMEM (Gibco) containing 10% FBS (Sigma). The SCLC cell lines were obtained from ATCC. NCI-H748, NCI-H1963, NCI-H69, NCI-H889, NCI-H196, NCI-H226, and NCI-H209 cells were maintained with ATCC-formulated RPMI1640 medium containing 10% FBS (Sigma). NCI-H1882, NCI-H1105, and NCI-H2171 cells were maintained with ATCC-formulated DMEM/F12 cell culture media containing 10% FBS (Sigma). All cell lines were authenticated through STR profiling and tested bi-weekly for Mycoplasma by PCR. Cell lines were not passaged more than 20 times.
3
Cell Culture Conditions for Breast Cancer Cell Lines
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The human breast cancer cells MDA-MB 468, BT20, MDA-MB 231, MCF7, T47D, SK-BR-3, HEK-293 and mouse 4T1 breast cancer cells were obtained from the American Type Culture Collection. MDA-MB 468 and HEK-293 cells were maintained in DMEM (Sigma) supplemented with 2 μM L-glutamine (Sigma); BT20 cells were maintained in EMEM (Sigma) supplemented with 1 mM Sodium Pyurvate (Sigma); MDA-MB 231, MCF7, T47D, 4T1 and PBMCs were maintained in RPMI 1640 cell culture media (Sigma); SK-BR-3 cells were maintained in DMEM/F12 cell culture media (Sigma). All culture media were supplemented with 10% FBS and 1% Penicillin-Streptomycin (P+S) and cultures were grown at 37 °C with 5% CO2.
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