Mouse embryonic fibroblasts were procured from ATCC (American Type Culture Collection Centre, MEF-SCRC1040) and cultured in complete DMEM (Delbecco's Modified Eagle Medium-Gibco Life technologies). To prepare the complete medium, the following were added to DMEM: 1) 10% FBS (ATCC); 2) 1% Penicillin/Streptomycin (Cellgro); 3) 1% Non-essential amino acids (Gibco) and 4) 1% Sodium Pyruvate (Gibco). The media was filter sterilized by passing through a 0.22 μm polyethersulfone sterilizing filter (Corning) inside the biological safety cabinet and stored at 4 °C till further use. The medium was prewarmed at 37 °C before use.
Delbecco s modified eagle medium
Manufactured by Thermo Fisher Scientific
Dulbecco's Modified Eagle Medium (DMEM) is a cell culture media formulation used to support the growth of various cell types in vitro. It provides essential nutrients, vitamins, and amino acids required for cell proliferation and maintenance. DMEM is a commonly used basal medium in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Sw620, by Thermo Fisher Scientific (1 mentions)
Hct15, by Thermo Fisher Scientific (1 mentions)
Colo205, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Merck Group (1 mentions)
Ht 29, by Merck Group (1 mentions)
Hct116, by Merck Group (1 mentions)
Sw480, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Sw620, by American Type Culture Collection (1 mentions)
Hct15, by American Type Culture Collection (1 mentions)
2 protocols using delbecco s modified eagle medium
1
Culturing Mouse Embryonic Fibroblasts
2
Cultivation of Colon Cancer Cell Lines
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The human colon adenocarcinoma HT29, SW620, SW480, HCT15, LoVo, 293T, and 293FT cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). KM12 was from Japan and Colo-205 was from Shanghai Institute of Biochemistry and Cell Biology. HT29 and HCT116 cells were grown in McCoy's 5A medium (Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY). SW620 and SW480 cells were grown in L-15 (Gibco, Grand Island, NY) supplemented with 10% FBS. HCT15 and Colo-205 were grown in RPMI-1640 (Gibco, Grand Island, NY) supplemented with 4.5 g/L glucose, 0.11 g/L sodium pyruvate, and 10% FBS. LoVo was grown in F-12 (Gibco, Grand Island, NY) supplemented with 0.29 g/L glutamine and 10% FBS. KM12 was grown in RPMI-1640 (Gibco, Grand Island, NY) supplemented with 10% FBS. The 293FT Lentiviral Expression System and 293T were cultured in Delbecco's modified Eagle medium (Gibco, Grand Island, NY) supplemented with 10% FBS.
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