Subconfluent, actively growing cells were trypsinized, counted, and plated in a 50 μl volume of complete medium at 5000 cells per well in a 96-well Corning Costar 3595 flat-bottom plate. Stock hCG was diluted in a series of 5-fold dilutions in complete medium and 50 μl added into triplicate wells per concentration for final concentrations of 50, 10, 2, 0.4, and 0.08 IU per ml. Plates were incubated for 48 hr at which time the medium was removed and replaced with warmed 100 μl HyClone DMEM without Phenol Red plus 10% MTS reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Pierce Biotechnology, Rockford, IL) and read every 10 min in a Fluostar spectrophotometer according to kit instructions. MTS assays were performed 3 times in triplicate for each cell line tested.
Celltiter 96 aqueous one solution cell proliferation assay
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CellTiter 96 Aqueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The assay uses a novel tetrazolium compound (MTS) and an electron coupling reagent (phenazine ethosulfate, PES) to produce a colored formazan product that is soluble in tissue culture medium. The quantity of formazan product, as measured by the absorbance at 490 nm, is directly proportional to the number of living cells in culture.
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Lab products found in correlation
Multiskan, by Thermo Fisher Scientific (1 mentions)
Varioskan plusplate, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
L cysteine hydrochloride, by Thermo Fisher Scientific (1 mentions)
Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions)
Exendin 4 ex 4, by AnaSpec (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Itaq universal sybr green supermix, by Bio-Rad (1 mentions)
Rneasy plus mini kit, by Qiagen (1 mentions)
Qiazol lysis reagent, by Qiagen (1 mentions)
Insulin, by ProSpec (1 mentions)
Heparanase, by R&D Systems (1 mentions)
P selectin, by R&D Systems (1 mentions)
Heparin biotin, by Creative PEGWorks (1 mentions)
Fibroblast growth factor 2 (fgf2), by R&D Systems (1 mentions)
7 protocols using celltiter 96 aqueous one solution cell proliferation assay
1
Dose-Dependent Cell Proliferation Assay
2
Dose-Dependent Cell Proliferation Assay
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Subconfluent, actively growing cells were trypsinized, counted, and plated in a 50 μl volume of complete medium at 5000 cells per well in a 96-well Corning Costar 3595 flat-bottom plate. Stock hCG was diluted in a series of 5-fold dilutions in complete medium and 50 μl added into triplicate wells per concentration for final concentrations of 50, 10, 2, 0.4, and 0.08 IU per ml. Plates were incubated for 48 hr at which time the medium was removed and replaced with warmed 100 μl HyClone DMEM without Phenol Red plus 10% MTS reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Pierce Biotechnology, Rockford, IL) and read every 10 min in a Fluostar spectrophotometer according to kit instructions. MTS assays were performed 3 times in triplicate for each cell line tested.
3
Cytotoxicity Evaluation of Chitosan and COSs
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The cytotoxicity of chitosan and COSs against MCF-7, HepG2, HeLa-6, and 3T3 was determined. The cell proliferation assay was conducted using the CellTiter 96™ Aqueous One Solution Cell Proliferation Assay.[22 (link)23 (link)] The absorbance was then read at a wavelength of 490 nm using an ELISA reader (Multiskan, Thermo Fisher, USA).
4
Cell Proliferation Assay of eDHFR Cells
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HEK293T-eDHFR-YFP and primary human eDHFR-FLAG T cells were plated in 96 well plates at approximately 40 E 5 cells per well in complete media. After 24 h, cells were treated with 7c for an additional 24 or 48 h. Then using the Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay, absorbance was measured by plate reader (ThermoFisher Varioskan Plusplate) at 490 nm.
5
Cell Proliferation Assay Protocol
6
Halloysite Nanotubes for Stem Cell Delivery
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Poly(caprolactone) (PCL; Mw-80kD), cellulose acetate (CA; Mw-50kD), halloysite nanotubes (HNTs), fluorescein isothiocyanate (FITC), papain, other salts, and analytical grade solvents were purchased from Sigma-Aldrich (St. Louis, MO). Human mesenchymal stem cells were purchased from RoosterBio Inc. (Frederick, MD) and Dulbecco's minimum essential medium (DMEM) media from Lonza Bioscience (Morrisville, NC). All cell culture supplies including pen-strep (P/S), fetal bovine serum (FBS), dialysis bags, l -cysteine hydrochloride, 0.25% trypsin EDTA, Quant-iT™ PicoGreen™ dsDNA Assay Kit, and Cell Titer 96® AQueous One Solution Cell Proliferation Assay were purchased from Fisher Scientific (Fair Lawn, NJ). QIAzol Lysis Reagent and RNeasy Plus Mini kit were purchased from QIAGEN Inc. (Germantown, MD) while iScript cDNA Synthesis Kit and iTaq Universal SYBR Green Supermix were purchased from Bio-Rad (Hercules, CA). Exendin-4 (Ex-4) was purchased from AnaSpec Inc. (Fremont, CA), and insulin was purchased from ProSpec Inc. (East Brunswick, NJ).
7
Heparanase Inhibition Assay Protocol
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