SK‐Hep1 cells were serum‐starved with 1% FBS for 16 hours before treatments. All treatments were performed in serum‐free DMEM medium. Celecoxib (1, 5, 10 μM, Selleck Chemical), 2,5‐Dimethyl‐Celecoxib (DMC, a structural analogue of Celecoxib with no COX‐2‐inhibiting activity, 1, 5, 10 μM, Sigma‐Aldrich), PGE2 (0.2, 1, 5 μM, MedChem Express), E‐prostanoid receptor 2 (EP2) antagonist (TG4‐155, 10 μM, MedChem Express), and ERK1/2 inhibitor (AZD6244, 1 μM, Selleck Chemical) were dissolved in dimethyl sulfoxide (DMSO, Sigma‐Aldrich). The final concentration of DMSO was 0.1% in the treated cells.
2 5 dimethyl celecoxib
2,5-dimethyl-celecoxib is a chemical compound used in laboratory research. It is a derivative of the drug celecoxib, which is a selective cyclooxygenase-2 (COX-2) inhibitor. The core function of 2,5-dimethyl-celecoxib is to serve as a research tool for studying the effects of COX-2 inhibition, but no further details on its intended use can be provided in an unbiased and factual manner.
Lab products found in correlation
3 protocols using 2 5 dimethyl celecoxib
Immortalized Human Liver Sinusoidal Endothelial Cells
SK‐Hep1 cells were serum‐starved with 1% FBS for 16 hours before treatments. All treatments were performed in serum‐free DMEM medium. Celecoxib (1, 5, 10 μM, Selleck Chemical), 2,5‐Dimethyl‐Celecoxib (DMC, a structural analogue of Celecoxib with no COX‐2‐inhibiting activity, 1, 5, 10 μM, Sigma‐Aldrich), PGE2 (0.2, 1, 5 μM, MedChem Express), E‐prostanoid receptor 2 (EP2) antagonist (TG4‐155, 10 μM, MedChem Express), and ERK1/2 inhibitor (AZD6244, 1 μM, Selleck Chemical) were dissolved in dimethyl sulfoxide (DMSO, Sigma‐Aldrich). The final concentration of DMSO was 0.1% in the treated cells.
Antibody and Chemical Reagents in Cell Studies
Transfection and Screening of Hepatoblastoma Cell Lines
The cells were seeded at a density of 5 × 105 cells/well, and DNA transfection was performed in a six-well plate using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer's instructions. The transfection ratio of the Donor vector PX459 (donated by the Key Laboratory of Ministry of Education for Gastrointestinal Cancer of Fujian Medical University): sgRNA vector was 3:1, and the co-transfected cells were screened for 2 week using 1 mg/mL G418. The red fluorescent PE intensity (the signal intensity of PE channel) of the reporter cells was detected using the BD Accuri C6 PlusTM platform. The materials used in the transfection experiments were interleukin-1β (IL-1β, ab9617, Abcam, Cambridge, UK) and small molecule inhibitors MK-886 and MF63 (Cayman Chemical, Ann Arbor, MI), 1,3-benzoxazol-5-amine and 2,5-dimethyl-celecoxib (Sigma-Aldrich, St. Louis, MO).
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