After 7 days in culture, cytokines were added to the complete culture medium to induce macrophage polarization. To induce M1, inflammatory macrophages, M0 (resting macrophages) were treated with 20 ng/mL of canine IFN-γ (R&D Systems Inc., Minneapolis, MN), while cells were treated with 20 ng/mL each of canine IL-13 and IL-4 (R&D Systems Inc., Minneapolis, MN) to induce M2, anti-inflammatory macrophages.
Canine ifn γ
Manufactured by R&D Systems
Sourced in Canada
Canine IFN-γ is a recombinant protein that represents the secreted form of interferon-gamma from the dog, Canis familiaris. Interferon-gamma is a cytokine that plays a key role in immune response and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using canine ifn γ
1
Macrophage Polarization: M1 and M2
2
Canine Biomarker Profiling After Transplant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The peripheral blood samples were collected from eight dogs at three specific time points: before transplantation (0 weeks); at suture removal (2 weeks); and at euthanasia (8 weeks). Blood was allowed to clot for 2 h at room temperature before centrifugation twice for 10 min at 1580 g. Sera were stored at −80°C until assayed. Serum C-reactive protein (CRP), serum interleukin-10 (IL-10), serum interferon-γ (IFN-γ), and serum cluster of differentiation 30 (CD30) were determined using commercially available canine-specific enzyme-linked immunosorbent assay (ELISA) kits: CRP canine high-sensitivity ELISA Kit (Helica Biosystems, Santa Ana, CA); Quantikine canine IL-10; canine IFN-γ (R&D Systems); and canine CD30 ELISA Kit (Novateinbiosciences, Cambridge, MA) (n = 7 or 8 dogs). All sera samples were analyzed in duplicate in accordance with the manufacturer's instructions.
3
Evaluating pSTAT3 Activation in Canine DH82 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The canine monocytic cell line DH82 (ATCC) was plated at a density of 100 000 cells per well in 96-well plates and grown overnight in MEM medium (Gibco Life Technologies) containing 15% FBS (Gibco Life Technologies) and 10 ng/mL canine IFNγ (R&D Systems). After 24 h, growth medium was replaced with serum-free MEM for 2 h. Test compound or vehicle control was then added for 1 h to generate an 11-point titration curve (ranging from 0.0001 to 10 μm ). Cells were treated with 1 μg/mL of canine IL-31 (in-house generated) for 5 min. Cytokine treatment was terminated by removing medium and adding AlphaScreen SureFire™ lysis buffer (Perkin Elmer). Activation of pSTAT3 was detected using Perkin Elmer AlphaScreen SureFire™ STAT3 p-Y705 kit. Data were expressed as mean Alpha Screen Signal units and then expressed as percent control. Dose–response data were then analyzed using a 4-parameter logistic equation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!