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P stat3

Manufactured by Vector Laboratories
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The P-STAT3 is a laboratory product manufactured by Vector Laboratories. It is used to detect and quantify the phosphorylation of the STAT3 protein, which is a key signaling molecule involved in various cellular processes. The product provides a reliable and efficient way to assess the activation status of STAT3 in biological samples.

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Lab products found in correlation

P ikk, by Cell Signaling Technology (2 mentions) P ikk, by Vector Laboratories (2 mentions) Dylight conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Anti esr1, by Santa Cruz Biotechnology (2 mentions) Rb 9017 p0, by Thermo Fisher Scientific (2 mentions) Anti pgr, by Thermo Fisher Scientific (2 mentions) Anti cdh1, by BD (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Mmp 9, by Cell Signaling Technology (2 mentions) P akt, by Cell Signaling Technology (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Anti cd31, by Abcam (2 mentions) Anti ki67, by BD (2 mentions) Pstat1 tyr701, by Cell Signaling Technology (1 mentions) Dab peroxidase substrate kit, by Vector Laboratories (1 mentions) Cx43 microscope, by Olympus (1 mentions) Vectorstain elite abc, by Vector Laboratories (1 mentions) P stat3 tyr705, by Cell Signaling Technology (1 mentions)

3 protocols using p stat3

1

Immunohistochemical Analysis of Mammary Tumors

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For immunohistochemical analysis of mammary tumors, tumors were dissected and fixed in buffered formalin solution for 48 hours. Tissues were embedded in paraffin, and 4-μm sections of the entire block were prepared. After deparaffinization and rehydration, sections were subjected to antigen retrieval in tris/EDTA buffer (pH 8.0) at 120°C for 5 min. Sections were blocked with 1.5% (v/v) horse (Ki67) or goat (p-STAT-1, p-STAT-3, and CD3) serum in PBS for 30 min at room temperature and incubated overnight (4°C) with p-STAT-1 (Tyr701) (1:500; Cell Signaling Technology), p-STAT-3 (Tyr705) (1:200; Cell Signaling Technology), Ki67- (1:400; clone #8D5, Cell Signaling Technology), or CD3-positive cells (1:200; Abcam). After washing with PBS, p-STAT-1, p-STAT-3, Ki67-, or CD3-positive cells were visualized using rabbit (p-STAT-1, p-STAT-3, and CD3) or mouse (Ki67) IgG VECTORSTAIN ABC Elite and DAB Peroxidase Substrate Kits (Vector Laboratories, UK). Sections were counterstained with hematoxylin and imaged on an Olympus CX43 microscope (Olympus, Tokyo, Japan).
Goh P.K., Wiede F., Zeissig M.N., Britt K.L., Liang S., Molloy T., Goode N., Xu R., Loi S., Muller M., Humbert P.O., McLean C, & Tiganis T. (2022). PTPN2 elicits cell autonomous and non–cell autonomous effects on antitumor immunity in triple-negative breast cancer. Science Advances, 8(8), eabk3338.
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2

Immunolocalization of Uterine Markers

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Immunolocalization of Ki67, CD31, ESR1, PGR, p-IKK, p-STAT3, CD68, CD163, COX2, iNOS, MMP9 and p-AKT was determined in cross-sections (5 μm) of paraffin-embedded uterine sections using specific primary antibodies and a Vectastain Elite ABC Kit (Vector laboratories, Burlingame, CA, USA) or DyLight-conjugated secondary antibody (Jackson ImmunoResearch Lab, West Grove, PA, USA). Antibodies used in these analyses were: anti-CDH1 (1:120 dilution, 610181, BD Biosciences, San Jose, CA, USA), anti-Ki67 (1:200 dilution, 550609, BD Biosciences), anti-CD31 (1:100 dilution, ab28364, Abcam, Cambridge, MA, USA), anti-ESR1 (1:100 dilution, sc-542, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-PGR (1:200 dilution, RB-9017-P0, Thermo Scientific, Rockford, IL, USA), p-IKK (1:150 dilution, 2697, Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (1:50 dilution, 9145, Cell Signaling Technology), CD68 (1:300 dilution, ab955, Abcam), CD163 (1:300 dilution, ab126756, Abcam), COX2 (1:50 dilution, RM-9121, Thermo Scientific), iNOS (1:50 dilution, 610333, BD Biosciences), MMP9 (1:50 dilution, 3852, Cell Signaling Technology), and p-AKT (1:200 dilution, 3787, Cell Signaling Technology).
Stodden G.R., Lindberg M.E., King M.L., Paquet M., MacLean JA I.I., Mann J.L., DeMayo F.J., Lydon J.P, & Hayashi K. (2014). Loss of Cdh1 and Trp53 in the uterus induces chronic inflammation with modification of tumor microenvironment. Oncogene, 34(19), 2471-2482.
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3

Immunolocalization of Uterine Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunolocalization of Ki67, CD31, ESR1, PGR, p-IKK, p-STAT3, CD68, CD163, COX2, iNOS, MMP9 and p-AKT was determined in cross-sections (5 μm) of paraffin-embedded uterine sections using specific primary antibodies and a Vectastain Elite ABC Kit (Vector laboratories, Burlingame, CA, USA) or DyLight-conjugated secondary antibody (Jackson ImmunoResearch Lab, West Grove, PA, USA). Antibodies used in these analyses were: anti-CDH1 (1:120 dilution, 610181, BD Biosciences, San Jose, CA, USA), anti-Ki67 (1:200 dilution, 550609, BD Biosciences), anti-CD31 (1:100 dilution, ab28364, Abcam, Cambridge, MA, USA), anti-ESR1 (1:100 dilution, sc-542, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-PGR (1:200 dilution, RB-9017-P0, Thermo Scientific, Rockford, IL, USA), p-IKK (1:150 dilution, 2697, Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (1:50 dilution, 9145, Cell Signaling Technology), CD68 (1:300 dilution, ab955, Abcam), CD163 (1:300 dilution, ab126756, Abcam), COX2 (1:50 dilution, RM-9121, Thermo Scientific), iNOS (1:50 dilution, 610333, BD Biosciences), MMP9 (1:50 dilution, 3852, Cell Signaling Technology), and p-AKT (1:200 dilution, 3787, Cell Signaling Technology).
Stodden G.R., Lindberg M.E., King M.L., Paquet M., MacLean JA I.I., Mann J.L., DeMayo F.J., Lydon J.P, & Hayashi K. (2014). Loss of Cdh1 and Trp53 in the uterus induces chronic inflammation with modification of tumor microenvironment. Oncogene, 34(19), 2471-2482.
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