Laminin b

Manufactured by Abcam

Laminin B is a protein that is a component of the extracellular matrix. It plays a role in cell attachment and migration.

Automatically generated - may contain errors

Lab products found in correlation

Horseradish peroxidase linked secondary anti rabbit or anti mouse antibody, by Bio-Rad (1 mentions) Histone h1, by Santa Cruz Biotechnology (1 mentions) Ph2ax, by Merck Group (1 mentions) Hif 1α, by Fortis Life Sciences (1 mentions) Parp 1, by Enzo Life Sciences (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Ecl system, by Cytiva (1 mentions) Hdac1, by Abcam (1 mentions) Vinculin, by Merck Group (1 mentions) H3k4me2, by Abcam (1 mentions) H3k4me1, by Abcam (1 mentions) Zmym3, by Abcam (1 mentions) Gfi 1, by Santa Cruz Biotechnology (1 mentions) H3k4me3, by Active Motif (1 mentions) Chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit igg secondary antibody, by Merck Group (1 mentions) Anti gr, by Abcam (1 mentions) Anti c jun, by Abcam (1 mentions) Anti stat4, by Santa Cruz Biotechnology (1 mentions) Anti nf κb p65, by Abcam (1 mentions)

Close spelling variants of this product

Laminin

4 protocols using laminin b

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in an ice-cold lysis buffer containing 50 mM Tris-Cl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 1% TritonX-100, 1 mM PMSF, 1 μg/ml leupeptin, and 1 μg/ml pepstain A. The protein concentration in the supernatant was determined using a BCA protein assay (Pierce, Rockford, IL, USA). Samples were subjected to 8-12% SDS-polyacrylamide gel electrophoresis, and the separated proteins were electrophoretically transferred to nitrocellulose membranes. Blots were incubated with the primary antibody against IRF1 (1: 1000, Santa Cruz Biotechnology), LC3B (1:1000, CST), Bnip3 (1:1000, CST), Tubulin (1:3000, Sigma), AIF (1: 1000, CST), coxIV (1: 1000, Calbiocam), Laminin B (1: 1000, Abcam). The membranes were then incubated with horseradish peroxidase-linked secondary anti-rabbit or anti-mouse antibodies (Bio-Rad).

Liang J., Piao Y., Henry V., Tiao N, & de Groot J.F. (2015). Interferon-regulatory factor-1 (IRF1) regulates bevacizumab induced autophagy. Oncotarget, 6(31), 31479-31492.

+ Open protocol

+ Expand

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Western Blot analysis, cells were plated in six-well plates at a density of 2.5 × 10⁵, lysed using TRE buffer and then the whole cell extract was sonicated, resuspended, and boiled for 5 min in modified Laemli charge buffer (250 mM Tris-HCl (pH 75), glycerol 20%, SDS 10%, 1,4 M of mercaptoethanol and 1% blue bromophenol). Samples were transferred to a nitrocellulose membrane through wet transfer (Amersham Biosciences). Proteins were then visualized using the ECL system (Amersham Biosciences) after using specific antibodies for: HIF-1α (Bethyl), GST antibodies (Bethyl), Tubulin (Sigma-Aldrich), Actin (Sigma-Aldrich), Myc-tag (Cell Signaling Technology), Poly(ADP-ribose) (Trevigen), PARP-1 (Enzo), RPA (Cell Signaling), p-RPA (Bethyl), P53 (Santa Cruz), p-P53 (Millipore), H2AX (Millipore), p-H2AX (Millipore), Histone H1 (Santa Cruz), Laminin B (Abcam).

Martí J.M., Garcia-Diaz A., Delgado-Bellido D., O'Valle F., González-Flores A., Carlevaris O., Rodríguez-Vargas J.M., Amé J.C., Dantzer F., King G.L., Dziedzic K., Berra E., de Álava E., Amaral A.T., Hammond E.M, & Oliver F.J. (2021). Selective modulation by PARP-1 of HIF-1α-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions. Redox Biology, 41, 101885.

+ Open protocol

+ Expand

Antibody Panel for Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for Western blot analyses were as follows: actin (Sigma-Aldrich, no. A4700, clone AC-40), GFI1 (Santa Cruz Biotechnology, sc-376949, clone B9), GSE1 (Proteintech, no. 24947-I-AP), H3 (Abcam, no. 1791), H3K4me1 (Abcam, no. 8895), H3K4me2 (Abcam, no. 32356), H3K4me3 (Active Motif, no. 39159), HDAC1 (Abcam, no. 7028), HMG20B (Proteintech, no. 14582-1-AP), laminin B (Abcam, no. 16048), LSD1 (Cell Signaling Technology, no. 2139 and Abcam, no. 17721), RAR (Santa Cruz Biotechnology, sc-551), tubulin (Santa Cruz Biotechnology, sc-32356), vinculin (Sigma-Aldrich, no. V9131, clone hVIN-1), and ZMYM3 (Abcam, no. 106626).

Ravasio R., Ceccacci E., Nicosia L., Hosseini A., Rossi P.L., Barozzi I., Fornasari L., Zuffo R.D., Valente S., Fioravanti R., Mercurio C., Varasi M., Mattevi A., Mai A., Pavesi G., Bonaldi T, & Minucci S. (2020). Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid–induced differentiation. Science Advances, 6(15), eaax2746.

+ Open protocol

+ Expand

Western Blot Analysis of NK92 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Western blot analysis, nuclear and cytoplasmic compartments of NK92 cells were extracted from 1–3 × 10⁶ cells via the Fermentas ProteoJET Cytoplasmic and Nuclear Protein Extraction protocol (Fermentas, Burlington, ON). Nuclei were lysed using Nuclear Lysis Buffer (Fermentas, Burlington, ON) and both lysed nuclei and cytoplasm were resuspended in Laemmlis SDS-Sample Buffer (4×) (Boston Bioproducts, Boston, MA). Samples were boiled for 10 minutes and proteins separated by electrophoresis with a 12% agarose gel and transferred to a nitrocellulose membrane for blotting. Proteins were visualized with anti-NF-κB p65 (Abcam, Cambridge, MA), anti-cJun (Abcam, Cambridge, MA), anti-STAT4 (Santa Cruz, Santa Cruz, CA), anti-GR (Abcam, Cambridge, MA) and HRP conjugated goat anti-rabbit IgG secondary antibody (Millipore, Temecula, CA) and chemiluminescence reagent (ThermoScientific, Rockford, IL). Quality of nuclear and cytoplasmic compartment extraction was determined by Laminin B and GAPDH, respectively (Abcam, Cambridge, MA). Blot density was quantified using Image J software.

Eddy J.L., Krukowski K., Janusek L, & Mathews H.L. (2014). Glucocorticoids regulate natural killer cell function epigenetically. Cellular immunology, 290(1), 120-130.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!