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2 protocols using cis permethrin

1

Insecticide Screening for Voltage-Sensitive Sodium Channels

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A total of 20 insecticides that target the VSSC and two synergists were used in this study: acrinathrin (88.7%, Chem Service, West Chester, PA, USA), bioallethrin (3.8% cis, 95.7% trans, Chem Service), bioresmethrin (99.5%, Chem Service), cispermethrin (99%, Chem Service), cyfluthrin (98%, Bayer CropScience, Leverkusen, Germany), cyhalothrin (90.2%, ICI Americas, Wilmington, DE, USA), cypermethrin (94.6%, ICI Americas), DDT (1,1‐bis(4‐chlorophenyl)‐2,2,2‐trichloroethane) (98%, Sigma‐Aldrich, St. Louis, MO, USA), deltamethrin (99.5%, Chem Service), diethyl maleate (DEM) (97%, Sigma‐Aldrich), etofenprox (96.3%, Mitsui Toatsu, Tokyo, Japan), 1R‐trans fenfluthrin (100%, Bayer CropScience), fenpropathrin (99.5%, Chem Service), flumethrin (100%, Bayer CropScience), indoxacarb (DPX‐MP062, 100%), and its bioactive metabolite DCJW, (98%, DuPont, Wilmington, DE, USA), tau‐fluvalinate (93.8%, Chem Service), permethrin (99.5% pure, 24.1% cis, 75.8% trans, Chem Service), piperonyl butoxide (PBO) (90%, Sigma‐Aldrich), tefluthrin (96%, Sigma‐Aldrich), transfluthrin (99.5%, Chem Service) and trans‐permethrin (99% FMC, Philadelphia, PA, USA). All insecticides were diluted in acetone (VWR, Radnor, PA, USA).
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2

Permethrin Isomer Characterization

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Cis-permethrin (3-phenoxybenzyl(1RS)-cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate, 99.4% purity) and trans-permethrin (3-phenoxybenzyl (1RS)-trans-3-(2,2dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate, 99% purity) were obtained from ChemService (West Chester, USA) and Dr. Ehrenstorfer (Augsburg, Germany), respectively. The internal standards cis-[ 13 C 6 ]permethrin (98% purity) and trans-[ 13 C 2 ]DCCA (98% purity) were purchased from LGC standard (Cambridge Isotope Laboratories, USA). Corn oil was acquired from Sygma-Aldrich (St Quentin Fallavier, France).
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