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Mac 1

Manufactured by R&D Systems
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The Mac-1 is a laboratory instrument designed for the detection and analysis of specific cellular markers. It utilizes flow cytometry technology to accurately identify and quantify the presence of target molecules on the surface of cells. The core function of the Mac-1 is to provide researchers with a reliable and efficient tool for immunophenotyping and cell characterization.

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Lab products found in correlation

Tnf α, by Enzo Life Sciences (1 mentions) Tnf r1 fc, by Enzo Life Sciences (1 mentions) Megacd40l, by Enzo Life Sciences (1 mentions)

Close spelling variants of this product

Recombinant Mac-1

2 protocols using mac 1

1

Modulating Phagocytic Killing Using Mac-1

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VCM prepared from pooled lavage fluid from naive C3H/HeN mice was pretreated with recombinant mouse Mac-1 (integrin αMβ2; R&D Systems) at concentrations ranging from 0.24 to 24 µg/ml for 1 h at 37°C. Mac-1-treated VCM was subjected to sterile filtration and used immediately in the PMN killing assay. Controls included untreated VCM and standard RPMI medium.
Yano J., Noverr M.C, & Fidel PL J.r. (2017). Vaginal Heparan Sulfate Linked to Neutrophil Dysfunction in the Acute Inflammatory Response Associated with Experimental Vulvovaginal Candidiasis. mBio, 8(2), e00211-17.
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2

Inhibiting CD154-Mediated Inflammation

Cited 1 time
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Chemicals and reagents used were obtained from Sigma-Aldrich (St. Louis, MO) except as indicated. Direct red 80 was from Santa Cruz Biotechnology (Dallas, TX). Recombinant receptors (hCD40:Fc, CD40:COMP, and TNF-R1:Fc) and tagged ligands (hCD154, hCD154:Fc, MegaCD40L, and TNF-α), were obtained from Enzo Life Sciences (San Diego, CA). Mac-1 (recombinant human integrin αMβ2 protein) and the monoclonal anti-human CD154 (clone 40804) and anti-human TNF-α antibodies (clone 1825) were obtained from R&D Systems (Minneapolis, MN). Inhibitory peptides KGYY1519 (link) were purchased from New England Peptide (Gardner, MA, USA).
Chen J., Song Y., Bojadzic D., Tamayo-Garcia A., Landin A.M., Blomberg B.B, & Buchwald P. (2017). Small-Molecule Inhibitors of the CD40–CD40L Costimulatory Protein-Protein Interaction. Journal of medicinal chemistry, 60(21), 8906-8922.
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