Hpr conjugated anti mouse antibodies

Pichia pastoris strain GS115, Escherichia coli strain DH5α, and expression vector pPIC9 were purchased from Invitrogen (Life Technologies). MDBK and Daudi cells were obtained from Wuhan Boster. The plasmid pPIC9K-Kex-IFNα2b-Fcγ1 with the coding gene of fused human IFN-α2b and the wild-type human IgG1 Fc fragment was constructed and preserved in our laboratory [13 ]. WISH cells, the conventional IFN-α and the anti-IFN-α monoclonal antibody were obtained from Anke Biotechnology Group. HPR-conjugated anti-mouse antibodies were purchased from Cell Signaling. The 14-L fermenter (New Brunswick BioFlo115) used in pilot-scale fermentation was obtained from Eppendorf. A flex stand system with 0.45-μm hollow fiber membrane filtration cartridges and an AKTA Avant system with MabSelect and S-200 HR columns were obtained from GE Healthcare.

P. pastoris strain GS115, E. coli strain DH5α and expression vectors pPIC9 and pPICZα were purchased from Invitrogen (Life Technologies, Carlsbad, CA). Anti-Agkisacutacin monoclonal antibody 1B9 was obtained from Zhaoke Pharmaceutical (Hefei) Co. Ltd. as described previously.3 HPR-conjugated anti-mouse antibodies were purchased from Cell Signaling Technology (Beverly, MA). The 14 L fermentor (New Brunswick BioFlo 115) used in the pilot-scale fermentation process was obtained from Eppendorf (Enfield, CT). FlexStand Systems with 0.45 μm, 500 kDa and 10 kDa hollow-fibre membrane filtration cartridges were obtained from GE Healthcare (Piscataway, NJ), and the AKTA avant System with CM FF and S-100 HR columns was obtained from GE Healthcare (Uppsala, Sweden). The high-performance liquid chromatography (HPLC) system (LC-20AD) with a TSK3000SW 5 μm 250 mm × 4.6 mm column (Tosoh Co., Tokyo, Japan) was obtained from Shimadzu (Kyoto, Japan). The ELX800 microplate reader was obtained from Bio-Tek (Winooski, VT). The BD FACSVerse flow cytometer was obtained from BD BioSciences (Franklin Lakes, NJ). The analysis software FlowJo was obtained from Tree Star (San Carlos, CA).