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Glycanassure data analysis software version 2

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GlycanAssure Data Analysis Software Version 2.0 is a software application developed by Thermo Fisher Scientific for the analysis of glycan data. The software provides tools for processing, visualizing, and interpreting glycan data obtained from various analytical techniques.

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Lab products found in correlation

Glycanassure apts kit, by Thermo Fisher Scientific (5 mentions) Pierce protein g spin plate, by Thermo Fisher Scientific (1 mentions)

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GlycanAssure software Data Analysis software

5 protocols using glycanassure data analysis software version 2

1

IgG N-Glycan Profiling via CE-LIF

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Bulk IgG was purified from cryopreserved plasma using Pierce™ Protein G Spin Plates (ThermoFisher Scientific). N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (ThermoFisher Scientific). Labelled N-glycans were analyzed using a 3500 Genetic Analyzer capillary electrophoresis system. Relative abundance of N-glycan structures was quantified by calculating the area under the curve (AUC) of each glycan structure divided by total glycans using the Applied Biosystems GlycanAssure Data Analysis Software Version 2.0 (Supplemental Figure 1).
GIRON L.B., AZZONI L., YIN X., LYNN K.M., ROSS B.N., FAIR M., DAMRA M., SCIORILLO A.C., LIU Q., JACOBSON J.M., MOUNZER K., KOSTMAN J.R., ABDEL-MOHSEN M., MONTANER L.J, & PAPASAVVAS E. (2020). HCV modulates IgG glycosylation in HIV co-infected antiretroviral therapy suppressed individuals. AIDS (London, England), 34(10), 1461-1466.
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2

Quantitative IgG and Plasma N-Glycan Analysis

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For both plasma and bulk IgG, N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher), following the manufacturer’s protocol. Labeled N-glycans were analyzed using the 3500 Genetic Analyzer capillary electrophoresis system. IgG N-glycan samples were separated into 17 peaks and total plasma N-glycans into 25 peaks (structures and names are in Supplementary Figures 3 and 4, respectively). Relative abundance of N-glycan structures was quantified by calculating the area under the curve of each glycan structure divided by the total glycans using the Applied Biosystems GlycanAssure Data Analysis Software Version 2.0.
GIRON L.B., PAPASAVVAS E., AZZONI L., YIN X., ANZUREZ A., DAMRA M., MOUNZER K., KOSTMAN J.R., SANNE I., FIRNHABER C.S., TATENO H., LIU Q., MONTANER L.J, & ABDEL-MOHSEN M. (2020). Plasma and Antibody Glycomic Biomarkers of Time to HIV Rebound and Viral Setpoint. AIDS (London, England), 34(5), 681-686.
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3

Quantification of Plasma N-Glycans

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IgG was purified from 50 μl of plasma using the Pierce Protein G Spin Plate (Thermo Fisher catalog #45204). N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol. The labeled N-glycans were analyzed using the 3500 Genetic Analyzer capillary electrophoresis system. The relative abundance of N-glycan structures was quantified by calculating the area under the curve of each glycan structure divided by the total glycans using the Applied Biosystems GlycanAssure Data Analysis Software Version 2.0.
Giron L.B., Liu Q., Adeniji O.S., Yin X., Kannan T., Ding J., Lu D.Y., Langan S., Zhang J., Azevedo J.L., Hanna D.B., Ofotokun I., Lazar J., Fischl M.A., Haberlen S., Macatangay B., Adimora A.A., Jamieson B.D., Rinaldo C., Merenstein D., Roan N.R., Kutsch O., Gange S., Wolinsky S., Witt M., Post W.S., Kossenkov A., Landay A., Frank I., Tien P.C., Gross R., Brown T.T, & Abdel-Mohsen M. (2023). Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV Infection. bioRxiv.
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4

IgG and Plasma N-Glycan Analysis

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For both plasma and bulk IgG, N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher cat. A33952), following the manufacturer’s protocol. Labeled N-glycans were analyzed using the 3500 Genetic Analyzer capillary electrophoresis system. IgG N-glycan samples were separated into 22 peaks and total plasma N-glycans into 24 peaks. Relative abundance of N-glycan structures was quantified by calculating the area under the curve of each glycan structure divided by the total glycans using the Applied Biosystems GlycanAssure Data Analysis Software Version 2.0.
Giron L.B., Palmer C.S., Liu Q., Yin X., Papasavvas E., Sharaf R., Etemad B., Damra M., Goldman A.R., Tang H.Y., Johnston R., Mounzer K., Kostman J.R., Tebas P., Landay A., Montaner L.J., Jacobson J.M., Li J.Z, & Abdel-Mohsen M. (2021). Non-invasive plasma glycomic and metabolic biomarkers of post-treatment control of HIV. Nature Communications, 12, 3922.
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5

IgG and Plasma N-Glycan Analysis

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For both plasma and bulk IgG, N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher; catalog # A33952), following the manufacturer’s protocol. Labeled N-glycans were analyzed using the 3500 Genetic Analyzer capillary electrophoresis system. Total plasma N-glycans were separated into 24 peaks (Supplementary Table 3) and IgG N-glycans into 22 peaks (Supplementary Table 4). The relative abundance of N-glycan structures was quantified by calculating the area under the curve of each glycan structure divided by the total glycans using the Applied Biosystems GlycanAssure Data Analysis Software Version 2.0.
Giron L.B., Dweep H., Yin X., Wang H., Damra M., Goldman A.R., Gorman N., Palmer C.S., Tang H.Y., Shaikh M.W., Forsyth C.B., Balk R.A., Zilberstein N.F., Liu Q., Kossenkov A., Keshavarzian A., Landay A, & Abdel-Mohsen M. (2021). Plasma Markers of Disrupted Gut Permeability in Severe COVID-19 Patients. Frontiers in Immunology, 12, 686240.
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