Western blot assays were performed using established protocols with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), pFRS2 1:1000 (Cell Signaling 3864), anti-FRS2 (abcam ab10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967) and GAPDH 1:10000 (Santa Cruz sc-25778).
Fgf receptor 2 d4l2v
Manufactured by Cell Signaling Technology
FGF Receptor 2 (D4L2V) is a recombinant protein that corresponds to a portion of the human fibroblast growth factor receptor 2 (FGFR2) protein. It is designed for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Total mek1 2, by Cell Signaling Technology (2 mentions)
Phospho fgf receptor tyr653 654, by Cell Signaling Technology (2 mentions)
Phospho pi3 kinase p85 tyr458 p55 tyr199, by Cell Signaling Technology (2 mentions)
Total akt, by Cell Signaling Technology (2 mentions)
P44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions)
Phospho mek1 2, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Phospho akt ser473, by Cell Signaling Technology (2 mentions)
Total mapk, by Cell Signaling Technology (2 mentions)
Pi3 kinase p85 19h8, by Cell Signaling Technology (2 mentions)
Pfrs2, by Cell Signaling Technology (1 mentions)
Anti frs2, by Abcam (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Phospho frs2α tyr 196, by Cell Signaling Technology (1 mentions)
Phospho plc γ1 tyr783, by Cell Signaling Technology (1 mentions)
2 protocols using fgf receptor 2 d4l2v
1
Western Blot Antibody Profiles
2
Signaling Pathway Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot assays were carried out using established protocols and probed with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), phospho-PLCγ1 (Tyr783) 1:1000 (Cell Signaling 14008), PLCγ1 (D9H10) 1:1000 (Cell Signaling 5690), phospho-FRS2-α (Tyr196) 1:1000 (Cell Signaling 3864), FRS2 1:1000 (abcam 10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!