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Laser doppler flowmeter

Manufactured by ADInstruments
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The Laser Doppler Flowmeter is a non-invasive instrument used to measure blood flow and perfusion. It utilizes the Doppler effect to detect the movement of red blood cells, providing quantitative measurements of tissue microcirculation.

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Lab products found in correlation

No 1 filter paper, by Cytiva (2 mentions) Whatman, by Cytiva (2 mentions) Powerlab system, by ADInstruments (1 mentions) Thermistor, by ADInstruments (1 mentions) Homeothermic blanket system, by Harvard Apparatus (1 mentions)

5 protocols using laser doppler flowmeter

1

Monitoring Regional Cerebral Blood Flow

Cited 1 time
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To monitor relative changes in regional cerebral blood flow during ischemia and reperfusion we used a laser-Doppler flowmeter (ADInstruments) equipped with a pencil probe affixed to the animal’s skull [12 (link)]. Pre-ischemic, intra-ischemic and during first 10 min of recovery rCBF was recorded in both WT and CD38KO animals.
Long A., Park J.H., Klimova N., Fowler C., Loane D.J, & Kristian T. (2016). CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation. Neurochemical research, 42(1), 283-293.
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2

Thrombosis Inhibition by Phytochemicals

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Rats (n=8/group) were orally administered with PARMG, EARMG (125, 250 or 500 mg/kg) or aspirin (30 mg/kg) for 3 weeks. Forty min after the final administration, the animals were anesthetized by intramuscular injection of urethane (1 g/5 mL/kg), under constant maintenance of body temperature (36-37℃) using a heating pad. The right carotid artery of rats were exposed and detached away from the vagus nerve and surrounding tissues. Aortic blood flow rate was monitored with a laser Doppler flowmeter (AD Instruments, Colorado Springs, CO, USA). At the time point of 1 hour after the final administration, arterial thrombosis was induced by wrapping the artery with a Whatman No. 1 filter paper (3×5 mm) soaked with 70% FeCl3 solution near (5 mm anterior to) the flowmeter probe for 5 min. The blood flow was monitored for 40 min, and the time to occlusion was recorded. Animals were sacrificed at the time point of 40 min from the application of FeCl3, and the arteries were cut to observe the thrombus in the artery.
Ban Y.H., Cha Y., Choi J., An E.S., Lee J.Y., Han N.R., Seo D.W., Jung G., Jeong D.H., Rhee M.H., Choi E.K, & Kim Y.B. (2017). Blood flow-improving activity of methyl jasmonate-treated adventitious roots of mountain ginseng. Laboratory Animal Research, 33(2), 105-113.
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3

Comprehensive Cardiovascular Monitoring Protocol

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Electrocardiographic leads were placed to monitor the heart rate and rhythm. Blood pressure was tracked continuously using an automated finger cuff device (Nexfin, bmEye, Amsterdam, The Netherlands or Finapres Medical Systems, Amsterdam, The Netherlands). Also measured were forearm blood flow by impedance plethysmography (Hokanson, Issaquah, WA), fingertip microcirculatory flow using a laser-Doppler flowmeter (ADInstruments, Colorado Springs, CO), and fingertip skin temperature using a thermistor (ADInstruments). The physiological signals were recorded using a PowerLab system (ADInstruments) and analyzed by LabChart software bundled with the PowerLab system. Forearm vascular resistance (FVR) was calculated from the mean arterial pressure recorded using the finger cuff device divided by the concurrent forearm blood flow.
Goldstein D.S., Sullivan P., Corrales A., Isonaka R., Gelsomino J., Cherup J., Castillo G, & Holmes C. (2021). Multiple catechols in human plasma after drinking caffeinated or decaffeinated coffee. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1185, 122988.
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4

Perilla Oil Modulates Arterial Thrombosis

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Rats (n=10/group) were orally administered with perilla oil (0.5, 1 or 2 mL/kg) or aspirin (30 mg/kg) for 1 week. Forty min after the final administration, the animals were anesthesized by intramuscular injection of Zoletil® (1 mL/kg). Under constant maintenance of body temperature (36-37℃) using a heating pad, the right carotid artery of rats were exposed, and dissected away from the vagus nerve and surrounding tissues. Aortic blood flow rate was monitored with a laser Doppler flowmeter (AD Instruments, Colorado Springs, CO, USA). At the time point of 1 hour after the final administration, arterial thrombosis was induced by wrapping the artery with a Whatman No. 1 filter paper (3 mm in diameter) saturated with 35% FeCl3 solution near (5 mm anterior to) the flowmeter probe for 10 min [9 (link),20 (link)]. The blood flow was monitored for 90 min. A part of the animals (n=3/group) were sacrificed at the time point of 50 min from the application of FeCl3, and the arteries were cut to observe the thrombus in the artery.
Jang J.Y., Kim T.S., Cai J., Kim J., Kim Y., Shin K., Kim K.S., Lee S.P., Kang M.H., Choi E.K., Rhee M.H, & Kim Y.B. (2014). Perilla oil improves blood flow through inhibition of platelet aggregation and thrombus formation. Laboratory Animal Research, 30(1), 21-27.
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5

Rodent Anesthesia and Monitoring for MCAO

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Animals were anesthetized using isoflurane (induction: 4–5% in 30% oxygen – 70% nitrous oxide, maintanance: 1–2% in room air). Buprenorphine HCl (0.05 mg/kg; sc) and bupivacaine (8mg/kg; intraincisional) were injected to alleviate pain. Rectal temperature was monitored continously during the anesthesia period and maintained at 37.0 °C by a homeothermic blanket system (Harvard Apparatus, Holliston MA). Temporalis muscle temperature was monitored continously using a T-type thermocouple probe and pod (ADInstruments, Colorado Springs, CO) and kept at 36.0 °C by a heating lamp. The right femoral artery was cannulated for continuous ABP and HR monitoring (ADInstruments) as well as intermittent ABGs and pH measurements (Rapidpoint 300 blood gas system, Siemens Healthcare Diagnostics Inc., Tarrytown NY). Regional cerebral blood flow (rCBF) was recorded over the right parietal cortex (5 mm lateral and 1 mm posterior to bregma) using laser Doppler flowmeter (ADInstruments) to verify induction of MCAO, as described before.[6 (link), 15 (link)]
Ay I., Nasser R., Simon B, & Ay H. (2015). TRANSCUTANEOUS CERVICAL VAGUS NERVE STIMULATION AMELIORATES ACUTE ISCHEMIC INJURY IN RATS. Brain stimulation, 9(2), 166-173.
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