Anti akt sc 5298

Manufactured by Santa Cruz Biotechnology

Anti-Akt (sc-5298) is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Akt protein, which is a serine/threonine protein kinase that plays a key role in various cellular processes.

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Lab products found in correlation

Anti erk sc 514302, by Santa Cruz Biotechnology (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti parp, by BD (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti phospho fak antibodies, by Cell Signaling Technology (1 mentions) Anti vinculin, by Merck Group (1 mentions) Anti p70 s6 kinase, by Cell Signaling Technology (1 mentions) Normal mouse igg sc 2025, by Santa Cruz Biotechnology (1 mentions) Anti p21 op64, by Merck Group (1 mentions) Anti phospho akt t308, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-Akt (175 citations) Sc-5298 (15 citations) Akt (sc-5298) (5 citations)

3 protocols using anti akt sc 5298

Comprehensive EGFR and Signaling Pathway Antibodies Protocol

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Antibodies against EGFR (#4267), IGF-1R (#9750), IR (#3025), VEGF2 (#2479), p-AKT (#4060), p-ERK (#4370), AKT (#9272), ERK (#4695), TFEB (#4240), FOX3a (#2497 and #12829), EPCAM (#2929), actin (#4970) and LC3B (#3868 and #2775) were purchased from Cell Signaling Technology (Danvers, MA). Anti-PARP was purchased from BD Biosciences (San Jose, CA). Anti-tubulin was purchased from Sigma (St. Louis, MO). Anti-AKT (sc-5298) and anti-ERK (sc-514302) were purchased from Santa Cruz (Dallas, TX). Anti-VEGFR1 (32152) was purchased from Abcam (Cambridge, MA). CXCR1 (55450–1-AP) was purchased from Proteintech (Rosemont, IL). CXCR2 (PA1–31217) was purchased from Fisher (Waltham, MA). An anti-DIRAS3 murine monoclonal antibody (ID8) was generated in our laboratory. Doxycycline hyclate (DOX, D9891), Vascular Endothelial Growth factor (VEGF, V-7259), Interleukin-8 human (IL8, I1645), Insulin-Like Growth Factor-I human (IGF-1, I3769) and AKT 1/2 kinase inhibitor (A6730) were purchased from Sigma-Aldrich (St. Louis, MO). For MAPK inhibitors, U0126 (#9903), SB203500 (#5633) were purchased from Cell Signaling Technology and JNK inhibitor II was purchased from Calbiochem (Burlington, MA)

Mao W., Peters H.L., Sutton M.N., Orozco A.F., Pang L., Yang H., Lu Z, & Bast RC J.r. (2019). The Role of VEGF, IL-8 and IGF in Sustaining Autophagic DIRAS3-induced Dormant Ovarian Cancer Xenografts. Cancer, 125(8), 1267-1280.

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Monoclonal Antibodies for Hpa2 Protein

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All reagents were purchased from Sigma unless specified otherwise. Anti-Hpa2 monoclonal antibodies were raised against Hpa2 protein purified from the conditioned medium of Hpa2-transfected HEK293 cells, essentially as described (14 (link), 18 (link)). Briefly, Balb/C mice were immunized with the full-length Hpa2 protein. Hybridomas were obtained by routine procedures and were selected by ELISA using Hpa2 for coating. Several hybridomas that reacted positively with Hpa2 were selected for further characterization. Performance of the newly developed monoclonal antibodies is summarized in Table 1. Anti-syndecan-1 (sc-5632), anti-syndecan-4 (sc-12766), anti-Erk (sc-94), anti-phospho-Erk (sc-7383), anti-focal adhesion kinase (FAK; sc-932), and anti-Akt (sc-5298) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-phospho-Akt and anti-phospho-FAK antibodies were purchased from Cell Signaling (Danvers, MA). Anti-vinculin and anti-actin antibodies were purchased from Sigma.

Gross-Cohen M., Feld S., Arvatz G., Ilan N, & Vlodavsky I. (2021). Elucidating the Consequences of Heparan Sulfate Binding by Heparanase 2. Frontiers in Oncology, 10, 627463.

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Investigating Akt and p53 Signaling

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Anti-ASS1 (sc-46066) and anti-Akt (sc-5298) antibodies and normal mouse IgG (sc-2025) were purchased from Santa Cruz Biotechnology. Anti-p53 (OP43), anti-p53 (OP140), and anti-p21 (OP64) antibodies were purchased from Merck Millipore. Anti–β-tubulin (#2125), anti–phospho-Akt (S473) (#4060), anti–phospho-Akt (T308) (#5106), anti–phospho-p70 S6 kinase (S389) (#9205), and anti-p70 S6 kinase (#2708) were purchased from Cell Signaling Technology. pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330) was a gift from F. Zhang (Addgene plasmid #42230) (61 (link)).

Miyamoto T., Lo P.H., Saichi N., Ueda K., Hirata M., Tanikawa C, & Matsuda K. (2017). Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Science Advances, 3(5), e1603204.

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