Lowry s reagent

Manufactured by Merck Group

Sourced in Germany, India

Lowry's reagent is a chemical solution used in protein quantification assays. It is a combination of copper(II) sulfate and an alkaline tartrate solution, which reacts with the peptide bonds in proteins to produce a blue-colored complex. This complex can then be measured spectrophotometrically to determine the protein concentration in a sample.

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Lab products found in correlation

Divinyl sulfone, by Merck Group (1 mentions) L tyrosine, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Sodium salt, by Merck Group (1 mentions) N naphthyl ethylenediamine, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions)

3 protocols using lowry s reagent

Enzymatic Assay for Tyrosine Hydroxylase

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L-tyrosine, (S)-2-amino-3-(3,4-dihydroxyphenyl)propanoic acid (L-DOPA), hydroxylamine (HA), ascorbic acid (AH₂), Lowry’s reagent, bovine serum albumin, and divinyl sulfone were purchased from Sigma–Aldrich (Germany). The other chemicals, all of which were of analytical or ACS grade, were obtained from Avantor Performance Materials (Poland).

Cieńska M., Labus K., Lewańczuk M., Koźlecki T., Liesiene J, & Bryjak J. (2016). Effective L-Tyrosine Hydroxylation by Native and Immobilized Tyrosinase. PLoS ONE, 11(10), e0164213.

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Analytical Reagents for Biochemical Assays

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AB62 was donated by Boruta-Zachem (Poland). Lowry’s reagent and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) sodium salt (ABTS) were supplied by Sigma-Aldrich. Other reagents of analytical grade were purchased from POCH (Poland).

Lewańczuk M, & Bryjak J. (2015). Continuous Decolorization of Acid Blue 62 Solution in an Enzyme Membrane Reactor. Applied Biochemistry and Biotechnology, 177(1), 237-252.

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Standardizing Chemical Reagents Usage

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Analytical and laboratory grade chemicals and reagents were used in the present study. Sodium salt of valproic acid was taken from Sun Pharma Pvt. Ltd. (Gurugram, India). Evans blue, ethylene glycol tetra acetic acid (EGTA) was purchased from SISCO Research Laboratory Pvt. Limited, Mumbai, India. Lowry’s reagent, N-naphthyle-thylenediamine and 5, 5’-dithiobis (2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich (Bengaluru, India). Hydrogen peroxide and pyridine was obtained from Rankem Laboratories Pvt. Ltd. (Gurugram, India).

Kumar H., Diwan V, & Sharma B. (2022). Protective Effect of Nimodipine Against Valproic-acid Induced Biochemical and Behavioral Phenotypes of Autism. Clinical Psychopharmacology and Neuroscience, 20(4), 725-736.

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