Nkg2c

Manufactured by Miltenyi Biotec

Sourced in Germany

NKG2C is a cell surface receptor expressed on natural killer (NK) cells. It functions as an activating receptor that recognizes stress-induced ligands on target cells, contributing to the regulation of NK cell-mediated immune responses.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Heat inactivated fetal calf serum, by Merck Group (1 mentions) 7 aad, by BD (1 mentions) Perk1 2, by BD (1 mentions) Cd16 biotin, by BioLegend (1 mentions) Pakt s473, by BD (1 mentions) Pplcγ2, by BD (1 mentions) P slp76, by BD (1 mentions) Ki 67, by Miltenyi Biotec (1 mentions) Bcl 2, by BioLegend (1 mentions) Nkg2c, by R&D Systems (1 mentions) Kir2dl1 s1, by Beckman Coulter (1 mentions) Cd122, by BD (1 mentions) Lag 3, by Miltenyi Biotec (1 mentions) Kir2dl1, by R&D Systems (1 mentions) Kir2dl3, by R&D Systems (1 mentions) Igg2a, by BioLegend (1 mentions) Facslyric, by BD (1 mentions) 7 aad viability dye, by Beckman Coulter (1 mentions)

Spelling variants of this product

NKG2C-APC (4 citations) NKG2C-PE-Vio770 (REA205) (2 citations) NKG2C-PE (2 citations) PE-conjugated anti-NKG2C (2 citations) CD159c (NKG2C) PE (2 citations) Anti-NKG2C-APC (1 citations)

3 protocols using nkg2c

Multiparametric NK Cell Profiling

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Antibodies were purchased for CD16 biotin from BioLegend; LFA-1 open conformation isoform was from Abcam; pZAP/Syk, pS6, pSLP76, pAKT (S473), pPLCγ2, pERK1/2, and NF-κB pp65 were from BD; KIR2DL1/S1 was from Miltenyi; KIR2DL2/3/S2 was from Beckman Coulter; KIR3DL1/2 was from BioLegend; CD57 was from BioLegend; NKG2A was from Beckman Coulter; NKG2C was from Miltenyi; CD56 was from Beckman Coulter; CD16 was from BioLegend; 7AAD was from BD; dead-cell marker was from Life Technologies; and CD107a was from BioLegend. Pacific Orange and Blue Succinimidyl Ester were bought from Thermo Fisher Scientific. 5-Methylthioadenosine (MTA) and 3-deazaadenosine (3-Deaza) were purchased from Sigma-Aldrich, 5-azacytidine (5-Aza) was from Biomol/Cayman, and 2-chloroadenosine (CADO) and EPZ015666 (EZH) were from Sigma. Avidin was purchased from Sigma. K562 cell line from ATCC was cultured in RPMI 1640 media with antibiotics (penicillin/streptomycin; Invitrogen) and 10% heat-inactivated fetal calf serum (Sigma) at 37°C.

Jacobs B., Schlögl S., Strobl C.D., Völkl S., Stoll A., Mougiakakos D., Malmberg K.J., Mackensen A, & Aigner M. (2020). The Oncometabolite 5′-Deoxy-5′-Methylthioadenosine Blocks Multiple Signaling Pathways of NK Cell Activation. Frontiers in Immunology, 11, 2128.

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Comprehensive Immunophenotyping Protocol

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The following antibodies were used for flow cytometry: BD Bioscience: CD3 epsilon (UCHT1, SP34–2 and SK7), CD122 (Mik-β3), CD8a (RPA-T8), PLZF (RI7–809). CD107a (H4A3), IFN-γ (B27), KIR3DL1 (DX9), KIR2DL2/L3/S2 (CH-L), CD16 (3G8), CD57 (NK-1), HLA-DR (G46–6), HLA-C (DT9), and CD86 (FUN-1). Biolegend: CD2 (RPA-2.10), PD-1 (EH12.2H7), Bcl-2 (100), CD3 epsilon (SK7, OKT3, HIT3a and APA1/1), HLA-ABC (W6/32), NKp46 (9E2), IgG1 (MOPC-21), IgG2a (MOPC-173), CD5 (UCHT2), Lck (LCK-01), and Bcl11b (25E6). Beckman Coulter: CD56 (NKH-1), KIR2DL2/L3/S2 (GL183), KIR2DL1/S1 (EB6), NKp46 (BAB281), CD247 (TIA-2), CD69 (TP1.55.3). Miltenyi: NKG2A (REA110), NKG2C (REA205), PD-1(PD1.3.1.3), LAG-3 (REA351), CD58(TS2/9), Ki-67 (REA183), Bw4 (REA274), and TCRrd (11F2). R&D Systems: NKG2C (134591), KIR2DL1 (143211), and KIR2DL3 (180701). Millipore: FcεRI (FCABS400F), HCMV-IEA (8B1.2). ThemoFisher: LIR-1 (HP-F1), TCRab (WT13), and CD8b (SIDI8BEE). Abcam: CD3 gamma (EPR4517), CD3 delta (EP4426) and Bcl11b (25E6). Jackson Immunoresearch: Donkey anti-rabbit IgG (H+L). The following antibodies were used for western blot: Biolegend: CD3 epsilon (SK7), IgG1 (MOPC-21), β-actin (W16197A), CD247 (6B10.2), pLck (A18002D), Lck (LCK-01), CD2 (RPA-2.10), and CD16 (3G8); pCD247 (C415.9A, Santa Cruz Biotechnology), CD3 gamma (EPR4517, Abcam), and CD3 delta (EP4426, Abcam).

Wu Z., Lau C.M., Sottile R., Le Luduec J.B., Panjwani M.K., Conaty P.M., Srpan K., Laib Sampaio K., Mertens T., Adler S.P., Hill A.B., Barker J.N., Cheung N.K., Sun J.C, & Hsu K.C. (2021). Human Cytomegalovirus Infection Promotes Expansion of a Functionally Superior Cytoplasmic CD3+ NK Cell Subset with a Bcl11b-Regulated T Cell Signature. The Journal of Immunology Author Choice, 207(10), 2534-2544.

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Profiling SARS-CoV-2 Induced NK Cell Changes

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Peripheral blood mononuclear cells (PBMC) were isolated from whole blood samples of the patients at days 15 and 30 following detection of SARS-CoV-2, by density gradient centrifugations using HiSep™ LSM 1077 media. For surface staining, 0.5 × 10 6 cells were washed with phosphate-buffered saline (PBS) and stained with the following antibodies which were used for phenotypic analysis: CD3(APC-H7, SK-7) CD16 (PE-Cy7, B73.1), CD56 (APC R700, NCAM16.2), CD57 (BV605, NK-1), NKG2A (PE-Cy7, Z199), from BD Biosciences, (San Jose, CA) and NKG2C (PE, REA205) from Miltenyi Biotec, Germany.
Cells were then incubated for 30 minutes. Viability was assessed with 7-AAD viability dye (Beckman Coulter). For intracellular staining, cells were stained for IFN-gamma using monoclonal antibodies for interferon-gamma (IFN-g) (4S.B3) and perforin (Alexa647, DG9) (BD Biosciences) after fixation and permeabilization with appropriate buffer (BD Biosciences and e-biosciences, San Diego, CA, USA). Flow Cytometry was performed using 10 colour flow cytometry (BD FACS Lyrics) and the flow cytometry data was analyzed using FlowJo software (v10.6.2, FlowJo). ANK cells were defined as CD56dimNKG2C+NKG2A-CD57+ subset of NK cells.

Jaiswal S.R., Arunachalam J., Bhardwaj A., Saifullah A., Lakhchaura R., Soni M., Bhagawati G., & Chakrabarti S. (2021). Impact Of Adaptive Natural Killer Cells, KLRC2 Genotype and Cytomegalovirus Reactivation On Late Mortality In Patients With Severe Covid-19 Lung Disease.

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