The largest database of trusted experimental protocols

Nu j immuno compromised mice

Manufactured by Jackson ImmunoResearch

Nu/J immuno-compromised mice are a laboratory mouse model developed by Jackson ImmunoResearch. These mice have a genetic mutation that results in a deficiency of T and B cells, leading to an impaired immune system. The Nu/J mice can be used in various biomedical research applications that require an immunocompromised animal model.

Automatically generated - may contain errors

2 protocols using nu j immuno compromised mice

1

Xenograft Mouse Model for Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal experiments were approved by the LSU Health Sciences Center – New Orleans Institutional Animal Care and Use Committee and were in accordance with the NIH/NCI guidelines for use of animals in cancer research. Male Nu/J immuno-compromised mice (25–35 g) were purchased from Jackson Labs (www.jax.org; #002019) and housed in groups of 5 with ad libitum access to mouse chow and water. The mice were maintained in a temperature and humidity controlled colony with a 12/12 hr light/dark cycle. Xenografts of MDA-MB-231 cells were established by s.c. injection of 4–5 million cells suspended in 50% DMEM/50% matrigel (Corning, Inc., www.corning.com: #356237) into the dorsal trunk region between the scapulae.
+ Open protocol
+ Expand
2

Conditional CD200 Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice containing a loxP-flanked Cd200 conditional allele (Cd200fl) were re-derived from frozen sperm (B6NTac;B6N-Cd200tm1a(KOMP)Wtsi/H) (EUCOMM MRC Harwell Repository). Mutant mice exhibiting germline Cd200fl transmission were backcrossed ten generations onto a FVB/N genetic background (Taconic Biosciences). Epidermal-targeted Cd200 conditional null mice were generated by crossing Cd200fl/fl mice with Krt14Cre transgenic mice [36 (link)] (B6N.Cg-Tg(KRT14-cre)1Amc/J; The Jackson Laboratory) to generate Krt14Cre;Cd200fl/fl (EpiKO) mice (Supplemental Figure 1A). Wild-type animals used were FVB/N (Taconic Biosciences) and C57Bl/6 (Jackson Laboratories). Nu/J immunocompromised mice (The Jackson Laboratory) were chosen for orthotopic studies as they harbor the same MHC H2 haplotype, H-2Kq, as the FVB/N mouse strain. All animals were housed under pathogen-free conditions and received standard rodent chow and water ad libitum according the Institute of Comparative Medicine guidelines. All experiments involving mice were conducted under Columbia University IACUC-approved protocols.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!