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Cc 2551

Manufactured by Lonza
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Sourced in Switzerland

The CC-2551 is a high-performance centrifuge designed for laboratory use. It features a compact and durable construction, digital speed and time controls, and an included rotor. The centrifuge is capable of reaching speeds up to 15,000 RPM and can accommodate a variety of sample tubes and microplates. Its core function is to separate and concentrate sample components through centrifugal force.

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Lab products found in correlation

Mfm 223, by Leibniz Institute DSMZ (2 mentions) Cal 148, by Leibniz Institute DSMZ (2 mentions) Mda mb 231 htb 26, by American Type Culture Collection (1 mentions) Skbr3 htb 30, by American Type Culture Collection (1 mentions) T47d htb 133, by American Type Culture Collection (1 mentions) L glutamine, by GE Healthcare (1 mentions) Rpmi 1640, by GE Healthcare (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Mcf 7 htb 22, by American Type Culture Collection (1 mentions) Geneprint 10 system, by Promega (1 mentions) Hydrocortisone, by Lonza (1 mentions) Human epidermal growth factor (hegf), by Lonza (1 mentions) Insulin, by Lonza (1 mentions) Ga 1000, by Lonza (1 mentions) Bt474, by American Type Culture Collection (1 mentions) Zr 75 1, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions)

4 protocols using cc 2551

1

Culturing Osteosarcoma, Kidney, and Mammary Cells

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U2OS osteosarcoma cells (obtained directly from American Type Culture Collection, HTB-96) were maintained in McCoy’s 5A medium supplemented with 10% (v/v) FBS (Hyclone), 100 units/ml of penicillin, and 0.1 mg/ml streptomycin. 293T cells were maintained in DMEM supplemented with 10% (v/v) FBS (Hyclone), 100 units/ml penicillin, and 0.1 mg/ml streptomycin. hTERT-immortalized Human Mammary Epithelial Cells (HMECs) from a reduction mammoplasty were purchased directly from Lonza (CC-2551), immortalized with human telomerase, and maintained in MEGM (Lonza).
Sack L.M., Davoli T., Xu Q., Li M.Z, & Elledge S.J. (2016). Sources of Error in Mammalian Genetic Screens. G3: Genes|Genomes|Genetics, 6(9), 2781-2790.
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2

Culturing Primary and Immortalized Breast Cells

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Primary human breast epithelial HMEC cells (CC-2551, Lonza, Basel, Switzerland) and immortalized breast epithelial 184A1 cells (a kind gift from A/Prof. Darren Shafren, the University of Newcastle) were cultured in mammary epithelial basal medium (MEBM) supplemented with bovine pituitary extract (BPE) (0.4%), human epidermal growth factor (hEGF) (0.1%), hydrocortisone (0.1%), GA-1000 (0.1%), and insulin (0.1%) (Lonza). MCF7 (HTB-22, American Type Culture Collection (ATCC), Manassas VA, USA), T-47D (HTB-133, ATCC), MDA-MB-231 (HTB-26, ATCC), and SKBR3 (HTB-30, ATCC; a kind gift from A/Prof. Darren Shafren, the University of Newcastle) breast cancer cell lines were cultured in RPMI-1640 (GE Healthcare, Chicago, IL, USA) supplemented with 10% FBS (Sigma–Aldrich, St. Louis, MO, USA) and 2 mM L-glutamine (GE Healthcare). All cells were maintained at 37 °C with 5% CO2 and used within four years of purchase from ATCC or Lonza, or authenticated using the GenePrint 10 System (Promega, Madison, WI, USA) as per the manufacturer’s instructions, and DNA fragments were detected by the Australian Genome Research Facility (AGRF) (Melbourne, VIC, Australia).
Bond D.R., Kahl R., Brzozowski J.S., Jankowski H., Naudin C., Pariyar M., Avery-Kiejda K.A., Scarlett C.J., Boucheix C., Muller W.J., Ashman L.K., Cairns M.J., Roselli S, & Weidenhofer J. (2020). Tetraspanin CD9 is Regulated by miR-518f-5p and Functions in Breast Cell Migration and In Vivo Tumor Growth. Cancers, 12(4), 795.
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3

Culturing Human Mammary Epithelial Cells

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The human primary cell line (HMEC) was cultured in mammary epithelial cell growth medium (MEGM, Gibco) supplemented with growth factors (MEGM-SingleQuots CC-4136, Gibco) bovine pituitary extract (BPE), hydrocortisone, human epidermal growth factor (hEGF), insulin and gentamicin/amphotericin-B (37 °C, 7% CO2). These cells (CC-2551) were obtained from Lonza.
Isbel L., Iskar M., Durdu S., Weiss J., Grand R.S., Hietter-Pfeiffer E., Kozicka Z., Michael A.K., Burger L., Thomä N.H, & Schübeler D. (2023). Readout of histone methylation by Trim24 locally restricts chromatin opening by p53. Nature Structural & Molecular Biology, 30(7), 948-957.
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4

Comprehensive TNBC Cell Line Characterization

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Nine of the 12 TNBC cell lines, as well as the non-transformed, immortalized control breast cell line MCF10A were obtained from the American Type Culture Collection (ATCC, Manassas, VA 20110, USA). Excluding BT-20 and MDA-MB-231, which were received in 2012, the cell lines were received in 2011. MDA-MB-468, BT-474, and ZR-75-1 were obtained by the Cell Culture Facility at Fox Chase Cancer Center from ATCC as part of the NCI-60 panel. The NCI-60 panel was obtained in 2003. The two additional TNBC cell lines, CAL-148 and MFM-223, were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany) in 2011. The suppliers routinely authenticate the cell lines by short tandem repeat profiling though the cell lines were not authenticated by our laboratory. All cell lines were amplified and frozen within 2 months of receipt. Cell lines are periodically tested for Mycoplasma contamination using DAPI (4′,6-diamidino-2-phenylindole) to stain DNA. Two different primary human mammary epithelial cell (HMEC) isolates were obtained from GIBCO (Details A10565) and Lonza (CC-2551), respectively, in 2012. Culture media for the cell lines can be found in Supplementary Table S1.
Beatty A., Fink L.S., Singh T., Strigun A., Peter E., Ferrer C.M., Nicolas E., Cai K.Q., Moran T.P., Reginato M.J., Rennefahrt U, & Peterson J.R. (2017). Metabolite profiling reveals the glutathione biosynthetic pathway as a therapeutic target in triple negative breast cancer. Molecular cancer therapeutics, 17(1), 264-275.
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